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Hello, I have rearranged the slides as you asked me toThe values i got for Q.Y. I got them from the data in the excel you send me
Absorption and Fluorescence
Institute of Affiliation
Absorption spectroscopy measures the amount of light
absorbed by a molecule when the energy of the light matches
the energy difference between two electronic states.
Our experiment was on Carbazole.
Properties of Fluorescence
Fluorescence spectroscopy is a very efficient way which is used
to determine the concentration of an analyte solution based on its
Absorption and fluorescence spectroscopy are the heart of life
science laboratory techniques
Fluorescence has a number of properties which define
how it works.
Life time is the average time a molecule can stay in its
excited stated before emitting a photon and go to the
The range of the life time of a molecule is picosecond to
hundreds of nanoseconds
To get the life time of a molecule, you can use the time
domain method or frequency domain method.
The life time of the molecule is known by quenching,
polarization and FRET.
Carbazole is a heterocyclic compound that is aromatic with a tricyclic
structure of six benzene rings fused together on either side of a
nitrogen ring that is five membered (Yoshiyama, 2018).
The following are the biological properties of Carbazole:
permeability- Carbazole is able to penetrate inside cells
rapidly and cross all membrane. Carbazole accumulation in cells
that are overexpressing is very low. The overexpressing is where
the molecules are constantly excited and in cells Carbazole binds