power point slides

timer Asked: Jan 28th, 2019
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Question Description

I want you to do powerpoint slides about Absorption and fluorescence spectroscopy , I will give you spesfically what to put, and the the end of the ppt slide you'll calculate Quantum Yeild, so if you don't know how to do that then don't take the question

Tutor Answer

School: UCLA

Hey Pal, Attached find the assignmentKindly let me know if you need any revision.Thanks for giving me the opportunity to work with you
Hello, I have rearranged the slides as you asked me toThe values i got for Q.Y. I got them from the data in the excel you send me

Absorption and Fluorescence
Institute of Affiliation


Absorption spectroscopy measures the amount of light

absorbed by a molecule when the energy of the light matches
the energy difference between two electronic states.

Our experiment was on Carbazole.

Properties of Fluorescence

Fluorescence spectroscopy is a very efficient way which is used

to determine the concentration of an analyte solution based on its
fluorescent properties.

Absorption and fluorescence spectroscopy are the heart of life

science laboratory techniques

Fluorescence has a number of properties which define
how it works.

Life time is the average time a molecule can stay in its
excited stated before emitting a photon and go to the
ground state.

The range of the life time of a molecule is picosecond to
hundreds of nanoseconds

To get the life time of a molecule, you can use the time
domain method or frequency domain method.

The life time of the molecule is known by quenching,
polarization and FRET.


Carbazole is a heterocyclic compound that is aromatic with a tricyclic
structure of six benzene rings fused together on either side of a
nitrogen ring that is five membered (Yoshiyama, 2018).

The following are the biological properties of Carbazole:
 Cell

permeability- Carbazole is able to penetrate inside cells
rapidly and cross all membrane. Carbazole accumulation in cells
that are overexpressing is very low. The overexpressing is where
the molecules are constantly excited and in cells Carbazole binds
with DN...

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