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SPR is a phenomenon that allows real-time, label-free detection of biomolecular interactions. SPR occurs when polarized light strikes an electrically conducting surface at the interface between two media.
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Take a case of sillver-silica nanocomposite being prepared under high pressure using sputtering technique, those deposited at lower substrate temperature showed a narrow distribution of nanoparticles with nearly spherical shape. Increase in substrate temperature resulted in films with a non-uniform size and shape due to the agglomeration of the nanoparticles. This size and shape distribution has a profound effect on the optical absorbance spectra and results in a broad and asymmetric surface plasmon band.