Thank you for the opportunity to help you with your question! I will do my best to help you out with this question!
I would think that you would be completing two parts for your experiment. The first part will be focusing on the effects of increasing pH, while the second will be focusing on the effects of increasing temperature. I will write out some ideas you could do, but feel free to edit or change it in any way you see fit. :)
The most commonly used enzyme is amylase, so that is the enzyme I will be referring to in this experiment example.
Hypothesis: Increasing the pH and temperature of the environment will directly affect the rate of enzyme activity of amylase.
Set up: In two pairs of test tubes (four all together), a sample of 1mL of starch solution was obtained by a pipette to one pair, while a sample of 1mL of maltose solution in the other pair. For each test tubs of maltose and starch, 5 drops of I2Kl was added; while in the other two test tubes, 5 drops of Benedict's reagent were added.
To test for temperature, three water baths were set up to 5, 25 and 40 degrees Celcieus respectively. A solution of pH 7 of starch solution was in all three water baths (so that pH would not affect the temperature results). 0.5mL was added to each water bath and observed for 1 minute, then 1mL of the temperature starch was added.
To test for pH we will get the four test tubes filled with different 1mL of pH (4, 5, 6, and 7) starch solution. 0.5ml of the amylase is added to each test tube and the immediately, one drop of each mixture is then transferred to the empty test tube, where one drop of 12Kl is added. This is repeated at one minute intervals.
*Above is what your set up will explain, so that the person reading it will be able to follow through the rest of your paper. One mistake is that I accidentally wrote it in past test (and it should be in present tense)!
-2 x 4 test tubes (or 4 test tubes if you clean them to reuse again)
-Instrument for measuring pH
-Beaker or pipette to measure
-Pencil and paper to record
Please let me know if you need any clarification. I'm always happy to answer your questions. Thank you very much and I hope that helps!
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