Need biology help with a Question regarding the Ames test on chili for Micro

timer Asked: Nov 18th, 2015
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Question Description

Andre performed an Ames test on some of his homemade chili. He found several revertant His+ colonies the next day. This result shows that Andre’s chili (Points : 4)

is carcinogenic in Salmonella.

Tutor Answer

School: University of Maryland

Thank you for the opportunity to help you with your question!

Here is how the Ames Test works. First of all, it is used for testing with Salmonella, so that gives us the two solutions with Salmonella as part of the answer as options. Next, suspected mutagenic substances are tested for their ability to stimulate backmutations by placement onto paper discs which are set on the surface of the minimal agar plates previously inoculated with the auxotroph. The test substance diffuses into the surrounding media and, if mutagenic, will induce back-mutations which will then allow growth into visible colonies. The more revertants observed near the test substance, relative to experimental controls, the more mutagenic the substance is likely to be. Therefore, the answer is "mutagenic in salmonella"

Please let me know if you need any clarification. I'm always happy to answer your questions.

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