Need health and medical help with a paper about sickle cell using zing-finger

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Methods_and_material.docx 

i need an outline of this.. of my part which is (material and methods).

Capstone_Methods_Outline.docx 


this is an example of an outline that i need to use on my paper

_Detailed_Outline_example (1).docx 


4 pages long and the rough draft is 6 pages long, so i you think about its 10 pages right there.

i need two seprate file.

1).  4 pages.(I need 4 pages explaining the procedure on a pregnant women 28-29 months to try and inject the baby so it won't come out with sickle cell) 4 page detailed outline. Like the example i sent you.

2). and 6 pages rough draft(The actual story on how the procedure will go down.The sign of consent everything f that's on the outline but in essay format).

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Capstone 5/8/15 Methods: Detailed Outline Fucoidan consumption associated with slowing the progression of breast cancer I. Participants A. Clinical single blinded study was approved to study the effects of seaweed consumption on breast cancer patients i. The Human Subjects Research and Review Board of West Coast University and Material Command (Los Angeles, California) ii. The Jackson Memorial Hospital (Miami, Florida) iii. West Coast University Institutional Review Boards iv. IRB number, 91-027 v. Clinical registration number WCU4166852 B. 18 diagnosed female breast cancer patients recently diagnosed in stages 3 and 4 referred to our clinical trial i. 9/18 had stage 3 breast cancer ii. 9/18 had stage 4 breast cancer II. C. Control group consisted of 10 female breast cancer patients in any stage of cancer D. Inclusion Criteria i. Breast cancer patients between the ages of 30-70 years old ii. Participants must have no allergies to seaweed, shellfish, iodine, or soy iii. Participants must not have been on hormone replacement therapy, radiation therapy, or chemotherapy within the previous six months of the study and no history of cancer (other than breast cancer) within the past twenty years iv. Participants must not have diabetes or gastrointestinal disorders v. Alcohol intake limited to less than one per week vi. No oral antibiotics taken within the last three months vii. Women agree to consume their normal diet, avoiding seaweeds and to continue their habitual use of medications, chemotherapy, vitamins and supplements during the study Description of breast cancer stage under investigation A. Stage 3 Breast Cancer i. Cancer extends beyond immediate region of the tumor ii. Invades nearby lymph nodes and muscles but has not spread to vital organs iii. Divided into three groups based on the size of the tumor: Stage 3A, 3B, and 3C iv. Stage 3A falls under three distinct descriptions 1 v. vi. B. III. a. Tumor is less than 2cm and has spread to 4-9 lymph nodes b. Tumor is larger than 5cm and cancer clusters found in lymph nodes c. Tumor is larger than 5cm and has spread to lymph nodes near the breastbone or underarm Stage 3B description a. Tumor size is variable and can be any size b. Cancer has spread to the chest wall or breast skin c. Cancer has spread to lymph nodes d. Evidence of swelling, inflammation or ulcers is visible e. Cancer has invaded at least 9 lymph nodes Stage 3C description a. No tumor or a tumor of variable size and cancer has invaded at least 10 or more lymph nodes b. No tumor or a tumor of variable size and cancer has spread to lymph nodes near collar bone c. No tumor or tumor of variable size and cancer has spread to lymph nodes near underarm or breastbone Stage 4 i. Cancer spread to other parts of the body, such as the brain, bones, lung and liver ii. This stage is considered incurable Study Design A. Clinical study spanned a total of 5 years from April 2015-April 2020 B. The ten control group breast cancer participants ingested a sugar placebo pill as their medication C. The remaining 18 breast cancer patients ingested 5 grams per day for a total of 10 500 mg capsules i. Seaweed capsules were obtained from a manufacturer based in Okinawa, Japan IV. Health and Mortality Surveillance A. Pre-evaluation tests were taken prior to the start of treatment to establish each participant’s baseline values i. Colony formation, MTT assay and xenograft to determine breast cancer cell numbers (EXPLAIN) ii. Blood tests- prior confirmatory results that diagnosed breast cancer patients a. Hormone receptor test- tests if the breast cancer cells have receptors for the hormone estrogen and progesterone b. HER2/neu test (ERBB2 test)- known as the human epidermal growth factor receptor 2 proto-oncogene. This is usually overexpressed and amplified in invasive breast tumor tissue 2 iii. Mammogram a. X-ray picture of the breast b. Used to check breast cancer who show no signs or symptoms of the disease c. Used to check for breast cancer progression d. Description of Procedure- breasts are compressed between two firm surfaces to spread out the breast tissue. The x-ray takes black and white images of the breast B. Daily seaweed capsule ingestion at 5 grams per day (ten 500 mg capsules per day) C. Every 6 months post treatment, colony formation, MTT assay, xenograft and cell number evaluation was done to assess improvement or no improvement in breast cancer tissue and cells i. Explain colony formation ii. Explain MTT assay iii. Above tests will determine cell numbers of breast tissue seen D. Every 3 months/90 days, CT scans were done to check for tumor growth i. Explain CT scan E. Monthly journal log that documented food intake, exercise/fitness routines and mood. This journal was given to case manager for review and discussion at monthly check up. F. Monthly support and rehab meeting to offer program support to breast cancer survivors G. Every four months breast cancer patients had a mammogram done to detect the presence or absence of cancer cell growth V. H. In the case that participants passed away, their death certificates were requested I. After five-year study, post evaluation tests were done to include colony formation assay and MTT assay on 24 and 48 hours to determine cell number differentiation Data Analysis A. Calculation of Participant Survival Rates i. 2/10 participants dropped out who were part of the placebo group due to non-compliance ii. 23 total survivors remained iii. 3/18 participants died. 2 died who was diagnosed with stage 4 cancer and 1 died who was diagnosed with stage 3 cancer B. Calculation of Participant Retention Rates i. 23/28 participants remained in the 5-year clinical study 3 C. Tumor Grading Description i. Use of Nottingham grading system - this grading system is a reference tool to describe tumor biology ii. Low-grade tumors (DESCRIBE) iii. High-grade tumors (DESCRIBE) D. Tumor Size Evaluation i. Comparison of 4T1 Wnt/B-Catenin (estrogen receptor positive), MDAMB 231 Wnt/B-Catenin (estrogen receptor positive), and MCF7 activation of capsase-8 (estrogen receptor positive signaling pathway) cancer cell lines to monitor the proliferation, cell differentiation or apoptosis of breast cancer cells ii. CT scan (CAT scan, also known as computerized tomography scan) to check growth of tumor a. x-ray technique to provide health care providers 2-dimensional cross sections of internal body parts iii. In Situ TUNEL Assay to check if fucoidan induced apoptosis in tumor tissue a. TUNEL assay tests DNA fragmentation by light microscopy REFERENCES About Breast Cancer. (n.d.). Retrieved May 5, 2015, from http://www.nationalbreastcancer.org/about-breast-cancer 4 Funahashi, H., Imai, T., Mase, T., Sekiya, M., Yokoi, K., Hayashi, H., Shibata, A., Hayashi, T., Nishikawa, M., Suda, N., Hibi, Y., Mizuno, Y., Tsukamura, K., Hayakawa, A. and Tanuma, S. (2001), Seaweed Prevents Breast Cancer? Japanese Journal of Cancer Research, 92: 483–487. doi: 10.1111/j.1349-7006.2001.tb01119.x Hsu, H., Lin, T., Hwang, P., Tseng, L. Chen, R., Tsao, S., & Hsu, J. (2013). Fucoidan induces changes in the epithelial to mesenchymal transition and decreases metastasis by enhancing ubiquitin-dependent TGFB receptor degradation in breast cancer. Carcinogenesis, 34(4), 874-884. Sekiya, M., Funahashi, H., Tsukamura, K., Imai, T., Hayakawa, A., Kiuchi, T., & Nakao, A. (2005). Intracellular signaling in the induction of apoptosis in a human breast cancer cell line by water extract of Mekau. International Journal Of Clinical Oncology, 10(2), 122-126. doi:10.1007/s10147-004-0469-2 Smyth, P.A. (2003). Role of iodine in antioxidant defense in thyroid and breast disease. Biofactors, 19(3/4), 121-130. Teas, J., Vena, S., Cone, D., & Irhimeh, M. (2013). The consumption of seaweed as a protective factor in the etiology of breast cancer: Proof of principle. Journal of Applied Phycology, 25(1),771-779. doi: 10.1007/s10811-012-9931-0 Vogel, V.G. (2015). Ongoing data from the breast cancer prevention trials: opportunity for breast cancer risk reduction. BMC Medicine, 13(1), 1-4. doi:10.1186/s12916-015-0300-0 Yang, Y., Nam, S., Kong, G., & Kim, M. (2010). A case–control study on seaweed consumption and the risk of breast cancer. British Journal of Nutrition, 103(9), 13451353. doi:10.1017/s0007114509993242 5 Capstone Methods Outline • Method A. Hook: In our stem cell transplant for SCD, stem cells are taken from the patient in utero, specialized to repair the defective gene, and implanted back into the fetus’s bone marrow. • #1 Subtopic: Overview of autologous stem cell transplant 1. Population criteria 2. Risks and benefits of autologous stem cell transplant 3. Surgical methods and tools of normal stem cell recovery and transplant • #2 Subtopic: Overview of pinpoint gene therapy 1. Zinc finger nucleases method 2. The study seeks to amend the change in the beta-globin chain so bone marrow stem cells produce ordinary, circular shaped blood cells 3. Utilized extraordinarily engineered enzymes • #3 Subtopic: Overview of fetal surgery 1. Risks associated with surgery 2. Minimally invasive fetal surgery that will be used in experiment 3. An ultrasound to see the baby movement 4. To inject the baby with zinc fingers to prevent sickle cell 5. Safety and complications. 6. Sign consent for procedure Capstone Discussion Outline Sickle cell disease is a hereditary blood disorder that is moved down through families. Both parents need to carry the sickle cell gene for their child to have it. In sickle cell disease, glutamic acid in beta chain of hemoglobin is supplanted by valine at position 6. It can bring about sickling of red blood cells, which lessens their flexibility and oxygen carrying capacity. Typical red blood cells are round or biconcave disc shaped and highly elastic. There is no generally accessible cure for Sickle Cell Disease. However, a little number of kids with Sickle Cell Disease have been cured through stem cell transplant. Stem cells are cells that can form into a wide range of cells in the body. They serve as a repair framework for the body. Stem cells are found in bone marrow. This is the elastic tissue inside a few bones, similar to your hip and thigh bones. Stem cells additionally are found in umbilical cord blood, the blood in the umbilical cord and placenta. This blood can be collected after the umbilical cord is cut during childbirth. In a stem cell transplant for SCD, stem cells taken from a healthy individual are put into a man with SCD. This system is extremely hazardous and can have genuine reactions, including death. The stem cell gene therapy system portrayed in the study seeks to amend the change in the betaglobin chain so bone marrow stem cells produce ordinary, circular shaped blood cells. The method utilized extraordinarily engineered enzymes, called zinc-finger nucleases, to wipe out the changed hereditary code and replace it with a corrected version that repairs the beta-globin mutation. The most recent gene correction method utilized by the group utilizes exceptional proteins, called zinc-finger nucleases, to truly cut out and remove the hurtful mutation, replacing it with an amended variant. So after gathered bone marrow stem cells from people with sickle cell disorder. These bone marrow stem cells would ordinarily give rse to sickle-shaped red blood cells. In any case, in this study, the group destroyed them with the zinc-finger nucleases keeping in mind the end goal to redress the mutation.
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Really helpful material, saved me a great deal of time.

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