Description
answer very shortly each question in the order they are found in the attached files.
"Students are required to keep a journal for objective write-ups. In order to be graded high for the objective journal, students must answer every objective clearly in writing and the writing must meet certain criteria (see below grading policy). Please make the journal a single word file that includes write-ups of the objectives of all topics."
The files are the objective questions for every topics. When you answer questions please include the question title.
And you do not need to answer too many words in every questions, two to four sentences are fine for me
Thank you so much
Objectives 1. Discuss the mechanism, list the materials and describe the procedures (steps) of PCR
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Explanation & Answer
Attached.
1. Discuss proper handling of specimen. Know when to reject specimen.
For proper handling of specimen to occur one must ensure that the patient is properly
prepared before collection, that a laboratory request form is duly filled with the correct
information, that the specimen is collected in sterile containers, that hands are washed
before and after collection and that proper transport procedures are applied. Instances
where specimen may be rejected include where it is not properly labelled, where the
wrong preservative and container were used and where the specimen is not sufficient.
2. Describe the requirements for holding and storing of various specimen including
sample temperature and time (e.g. what is the optimal conditions to store the DNA
in the blood for a week, what if the DNA is from microorganisms? What about
RNA? DNA Bank?
Storage of DNA for medium term is done at -20° C or -80° C depending on the duration
Since acidic conditions cause hydrolysis of DNA, DNA in the aqueous phase is stored
under slightly basic conditions in Tris: EDTA buffers or as a precipitate under ethanol(at
-80° C). DNA can be stored at 4°C or even RT without degradation, but samples should
be monitored for DNA concentration and evaporation for short periods.
3. Name the organization that has established standards used by laboratory
professionals when developing, implementing and performing molecular based
laboratory tests.
Clinical Laboratory Improvement Amendments (CLIA)
4. List basic components of molecular test performance. Explain and calculate
sensitivity, specificity (analytical and clinical specificity) accuracy and precision.
Molecular tests refers to methods used to collect and analyses data biological markers in
the genome and proteome in someone’s’ genetic code and how their cells express their
genes as proteins—by applyi...
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