Lonstar College Proteus Mirabilis Microbiology Laboratory Report

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Description

Microbiology Laboratory Research Report Instructions

Each student must write one research report on their assigned organism.The format for the report is outlined below.The report should be more than two pages in length, but a maximum of four pages long (not including pictures and references).All text should be 12 point font and single spaced with 1 inch margins.

Format for Research Reports - Each student will write his or her own research report based on the organism that they are assigned.The focus of the lab report will be how you would identify your particular microorganism.Each lab report should consist of the following sections that should be identified using the following word/wording:

Title – The title should be the name of the organism that is the subject of your report.

Introduction – A background on your microorganism covering things such as diseases it might be associated with and virulence factors (Please list virulence factors that the organism uses to promote the disease state i.e. Glycoprotein Spikes, ability to change genetically).

Identification – You should have the main body of the paper focus on how you would identify your microorganism, covering the following areas;

1) Cell Morphology – talk about staining procedures that can be used to identify your microorganism as well as what it looks like after the staining procedures are done i.e. after simple staining.

2) Gram (+) or Gram (-) – How you would use the gram staining procedure and what the outcome would be with your microorganism.

3) Special Stains – How and what specials stains you would use to identify your microorganism.

4) Other differential stains you might be able to use such as Acid-Fast Staining.

5) Colony Morphology – How you would set-up to look at colony morphology and what your bacteria would look like on the appropriate media it grows on.

6) Selective and Differential Media – What media you would use to identify your microorganism and what the outcome would be.

Summary – A summary paragraph about your microorganism as it relates to identification.

References – Please include a list of references you used to create your report using MLA citation style (Check this website for examples: http://www.library.cornell.edu/resrch/citmanage/ml... minimum of 3 references should be cited (at least one should be a reference different than your book or the internet). Please do not use Wikipedia or Youtube as a reference!

Terms - You must also properly utilize at least 5 terms that you have learned during this course in the report.Please use a change in format, such as color, bold, underline, italics etc. to indicate that the term refers to something you learned in class i.e. virulence factor, capsid, cell wall.

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Explanation & Answer

Please find attached the completed assignment. Let me know in case of any needed changes.Cheers.

Name 1

Name
Institutional Affiliation
Unit
Date
Proteus Mirabilis
Proteus Mirabilis was discovered by Gustav Hauser in 1885. The name was borrowed
from a character in Greek mythology that was good at changing shapes and evasion which is
a marker of this pathogen (Manos & Belas, 246). There are at least five Proteus species
which are opportunistic pathogens: Mirabilis, Vulgaris, Penneri, Myxofaciens and Hauseri.
Proteus Mirabilis is responsible for the majority of urinary tract infections. Proteus Mirabilis
is abundant in water and soil. It can lead to the development of kidney stones, obstruction and
renal failure. The pathogen can also cause infection and sepsis on wounds as well as
pneumonia. This pathogen is more closely attached to Catheter associated UTIs which are
common especially in people who remain catheterized for a good chunk of the time (Manos
& Belas, 248). Proteus Mirabilis can also reverse the impact of antibiotic treatment.
Identification
Cell Morphology
Proteus Mirabilis impacts by swarming motility just like is common for all Proteus
species. In Catheter Associated Urinary Tract Infections, formation of biofilm by way of
fimbriae and mitigated flagella allows urease activity which then leads to infection. Fimbriae
are the only virulence factor that is not expressed during differentiation like the others
(Norsworthy & Pearson, 306). The pathogen replicates and spreads. Proteases enhance action
of the pathogen. Simply put, swarming is controlled by sensation of environment and reaction
to chemical presence (Rather, 1065).
Simple staining is a procedure that easily reveals the morphology of bacteria. It uses a
single...


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