BIO 152 GCCCD ?Growth of Microorganisms in A Laboratory Exam

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zrx90

Science

bio 152

Grossmont Cuyamaca Community College District

BIO

Question Description

I’m working on a Biology exercise and need support.

Bio 152 Exam 1: Lab Stuff Study Guide

1. Be able to describe the steps involved in performing aseptic transfers (ex 1-4)

2. Define the following terms:

a. Culture

b. Medium

c. Inoculate

d. Contaminate

e. Pure culture

f.

Colony

g. sterile

3. Be able to locate the following on a compound microscope (see microscopy lab

supplement doc on Canvas):

a. Arm

b. Oculars

c. Course adjustment knob

d. Fine adjustment knob

e. Objective lenses

f.

Stage

g. Iris diaphragm

h. Condenser position adjustment knob

i.

Condenser

j.

Light source

k. Illumination control knob

l.

Base

4. Know how to calculate the total magnification for each of the objective lenses (10x, 20x,

40x, 100x) on our microscope (exercise 3-1 and microscopy lab supplement doc on

Canvas).

5. When given the Field of View (FOV) Diameter for a particular objective, be able to

estimate the size of a specimen (see microscopy lab supplement doc on Canvas).

6. Know how and when to clean the microscope, how to store the microscope, and how to

focus the microscope. (exercise 3-1 and microscopy lab supplement doc on Canvas).

7. What did you observe with the appearance of the “e” slide under the microscope?

When the stage moves to the right, the image moves _______? When the stage moves

toward you, the image moves ______? (exercise 3-1 p 149)

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Bio 152 Exam 1: Lab Stuff Study Guide
1. Be able to describe the steps involved in performing aseptic transfers (ex 1-4)
2. Define the following terms:
a. Culture
- refers to the microbes that grow and multiply in or on a culture medium
b. Medium
- A nutrient material prepared for the growth of microorganisms in a laboratory
c. Inoculate
-this refers to the process of introducing a microorganism or a suspension of microorganisms
into a culture medium
d. Contaminate
- this refers to the introduction of non-intended microbes. The microbes can be, bacteria,
yeasts, moulds, viruses, fungi, protozoa or even toxins and their by-products.
e. Pure cultureA pure culture, sometimes called axenic culture refers to the growth of a single type of
microbe in an environment free of any other kind of living thing
f. colony
This refers to the viable co...

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Cornell University

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