Science
General Chemistry Lab

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I need someone hlep me to do assignmnet in Chemistry Lab

Cranberry Data Table


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Data Table Stock Solution Conc. % v/v Volume of Stick Solution (mL) Concentration of Dilute solution % v/v Final Volume of Dilute Solution mL 4% 50.00 27% 8% 50.00 27% 10% 50.00 27% 12% 50.00 27% 16% 50.00 27% 20% 50.00 27% 7.41 Standards Volume of Juice Final Volume of Solution 4 % V/V 8 % V/V 10 % V/V 12% V/V 16% V/V 20% V/V Standards % v/v Absorbance Values 4 8 10 12 16 20 1 Line of Best Fit Equation: Concentration of Unknown: = % V/V 2 Experimental Procedure Calibration Curve of Dilutions of 100% Cranberry Juice The calibration curve of Standard cranberry juice (27%) will be used to help determine the concentration of cranberry products. The data collected from the dilutions of cranberry standard will be used to generate the calibration curve. The calibration standard will be prepared by preparing 50.00 ml of each of the following dilute solutions: 4%, 8%, 10%, 12%, 16%, and 20%. Example of Dilution using 27% Cranberry Standard Solution. Use the Dilution equation from the concentration lab exercise. Conc1 (Stock) X Vol1 (of Stock) = Conc2 (Dilute) X Vol2 (of dilute solution) Solution 1: Information Provided: Concentration of Stock: Volume of Stock: Concentration of Dilute Solution: Volume of Dilute Solution: 27% Unknown 4% 50.00 mL or 0.05000 L Enter the information into the equation from above: High Conc Low Conc 27% V/V * Needed = 4% * 0.0500 Liters Solve 4% V/V π‘†π‘‘π‘œπ‘π‘˜ π‘‰π‘œπ‘™π‘’π‘šπ‘’ = βˆ— 0.0500 πΏπ‘–π‘‘π‘’π‘Ÿπ‘  27% V/V 0.00741 Liters or 7.41 mL (V/V denotes the ratio of Volume cranberry juice to Volume Apple Juice) 1 Complete the following Dilution Table Base the volume transferred on the precision of a buret. Stock Solution Conc. Volume of Stock Concentration of Dilute % v/v Solution (mL) solution % v/v 27% 7.41 Final Volume of Dilute Solution mL 4% 50.00 27% 8% 50.00 27% 10% 50.00 27% 12% 50.00 27% 16% 50.00 27% 20% 50.00 1. Measuring the Absorbance of the Diluted Concentrations of 100% Cranberry Juice. Measure each cuvette at the Ξ»max, 520 nm. Concentration Absorbance of Dilute Cranberry solution % v/v 4 0.07 8 0.15 10 0.27 12 0.59 16 0.71 20 0.84 Unknown 0.163 % Conc Abs 4 8 10 12 16 20 0.07 0.15 0.27 0.59 0.71 0.84 Data entered in Excel should have no units As displayed above. 2 2. Graph the Data. Plot an absorbance vs. concentration graph is submitted on line. Use the data collected for the 4%, 8%, 10%, 12%, 16%, and 20% Solutions The graph should include the following: β–ͺ β–ͺ β–ͺ Data table Chart title Label both axis Equation of the line R2 value Note: The line for the best-fit equation may not go through zero, which is acceptable; this may occur because of random error effects, which are noticeable due to the small sample size. The graph you generate will be the calibration curve. Sample Graph & Data Calibration Curve for Cranberry Juice 2.50 2.00 Absorbance β–ͺ β–ͺ 1.50 y = 0.018x + 0.6 RΒ² = 0.9893 1.00 0.50 0.00 0 10 20 30 40 50 60 70 80 90 Cranberry Juice Concen. (%v/v) 3 3. Unknown Concentration: Measure the absorbance and calculate the concentration using the line of best-fit equation. Example: The numbers in the following problem are made up. Do not use them in your calculations. Use your data! Using the line of best-fit equation from the above graph, we can determine the concentration of any cranberry solution that has an absorbance within the range of our calibration curve absorbance values. (Sample Problem) Y (Abs) = 0.018 * X(Cranberry Conc.) +0.6 (Sample Problem) The y-axis represent the absorbance by the machine in response to the color of the solution. Darker color, Greater absorbance. If the unknown returned an Absorbance of 1.04 (my y value), I would calculate the concentration (my unknown x value) as follows: Y (Abs) = 0.018 βˆ— X(Cranberry Conc. ) + 0.6 1.014 (Abs) = 0.018 βˆ— X(Cranberry Conc. ) + 0.6 1.014 (Abs) βˆ’ 0.6 = X(Cranberry Conc. ) 0.018 The concentration of the unknown is 23 % V/V 4 Name: Last Name, First Name Week 14: Cranberry Lab Calculations Standards 4 % V/V 8 % V/V 10 % V/V 12% V/V 16% V/V 20% V/V Volume of Juice Final Vol of Solution Graph Concentration % V/V Absorbance ~4% ~8% ~10% ~12% ~16% ~20% Line of best fit R2 value y= R2 = Do NOT enter units into the cells 14: Cranberry Lab Concentration of standard: Unknown Absorbance: Unknown Concentration: Place Graph Here ...
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