Description
I need a power point about ~30 slides long (including pictures...) and one page summary includes the information below.
Selected enzyme: Monoamine oxidase (target of MAOI drugs)
1. The role of the enzyme in disease/industry
2. The mechanism of the enzyme
3. Description of drugs/inhibitors that target the enzyme
4. Any other relevant information that you think is important
I also need references:
-At least 2 primary or review articles from peer reviewed journals
-Provide a brief (2-3 sentences) summary for each reference on the information relevant to the project it will provide.
Explanation & Answer
Hi again :) Your presentation is attached. Please let me know if you have any questions. Otherwise, I hope you have a great rest of your day! 👋
Monoamine
Oxidase
NAME
DATE
COURSE
2
Outline
â–ª
Structure and physical properties
â–ª
Role in disease
â–ª
Enzyme mechanism of action
â–ª
Drugs and Inhibitors
â–ª
Review
Structure and Properties
4
Monoamine Oxidases (MAO) Overview
o
MAOs are a class of enzymes that catalyze the removal of amine groups using oxygen.
o
They are located on the outer membrane of the mitochondria.
o
They have a role in both breaking down food and in inactivating certain neurotransmitters.
o
There are two classes:
- MAO-A found in the liver and gastrointestinal tract.
- MAO-B found in blood platelets.
5
MAO Structure
o
MAOs contain a FAD binding domain and varied
substrate binding sites.
o
An FAD co-factor attaches to the enzyme’s cysteine to
allow for proper function.
o
MAOs contain both a hydrophobic substrate binding
domain and a membrane binding domain.
o
MAOs are found as dimers in solution. Each dimer is
made of 520 amino acids.
Protein Data Bank images for MAO A
and B with the FAD binding domain in
blue, substrate binding domain in red.
6
Physical Properties of MAOs
o
MAOs have both a hydrophobic region and an α-helix domain.
o
The hydrophobic region serves as the active site while the α-helix domain allows for attachment
to the outer membrane of the mitochondria.
o
There is also an aromatic cage that can hold the FAD co-factor molecule.
o
This three-dimensional structure gives MAOs many of their properties, such as solubility, and
gives them their function.
MAO Role in Disease
8
Normal Function of MAOs in Digestion
o
MAOs are vital to the breakdown of monoamines ingested in food.
o
They are part of the protein catalysis pathway and remove an amine group from a molecule,
creating a ketone and ammonia.
o
MAOs are especially important for the breakdown of tyramine, found in excess in cheese.
o
However, this is not the main role of MAOs as there are other enzymes in the digestive system
that p...
Review
Review
