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Chemistry Lab Math Type Question

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You need 4 micro litres of a 1/5 dilution of serum.  How much serum and how much diluent are needed to prepare the dilution?

Oct 18th, 2017

To prepare 1/5 dilution of serum 

  1. A 1:5 dilution of serum was made by adding 1 part serum to 4parts diluent to make a total of 5 parts.
  2. If 1.0 milliliter of serum is added to 4.0 milliliters of H20, a total volume of 5.0 milliliters is obtained.
And in this case we want 4 micro litres of a dilution of serum.
x+4x=4microlitres
5x=4
x=4/5
x=0.8 micro litres 
That implies we need 4 parts of diluent 
4x = 4*0.8 =3.2micro  litres
So we need 0.8 micro litres of serum and 3.2 micro litres of diluent to get  4 micro litres of a 1/5 dilution of serum.  


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