Description
In this first discussion, you will learn two important techniques commonly used in the microbiology lab. Proceed to the Virtual Interactive Bacteriology Laboratory and complete the "Gram Stain" and "Streak Plate" exercises. Click on the pdf that accompanies each activity, and then select "Open Module" to complete the exercises and become more familiar with how we culture and identify bacteria in the laboratory. Within the module you will read the "Description," and then the "Steps," before you select "Start," all from the main menu of the module. Keep your pdf open as you work through the exercises, so that you can refer back to the instructions. Once you have completed the exercises, respond to the following questions:
1) Why is it necessary to flame the inoculating loop each time you use it?
2) What part of the bacteria is "stained" by the Gram stain reagents?
3) What is Agar, and why is it used?
4) When "streaking" the bacterial culture onto the Agar plate, how does each successive "streak" establish a "pure culture"?
In this second Discussion, return to the Virtual Interactive Bacteriology Laboratory website, and complete the "Differential Media" lab exercises to prepare for your Case assignment, and participate in this Module's discussion. Remember to keep the pdf Module Instructions open as you work through the module media. Once you have completed the exercises, respond to the following questions:
1) What is the difference between "Selective Media" and "Differential Media?
2) Why is blood used in the "Hemolytic Reactions"? How is this useful when identifying bacteria?
3) How will this module help you with your assignments in the "VUMIE" laboratory program?
4.) Prions are very unusual pathogens. How do they compare with viruses and other microbial organism? What diseasesare they thought to cause?For our third discussion, return to the Virtual Interactive Bacteriology Laboratory . Complete the first four biochemical tests: Catalase test, Coagulase Test, Oxidase Test, and Rapid Strep A test (remember to keep a copy of the pdf instructions open as you work through each test). Once you have completed these tests, briefly summarize what you learned from each test, and the significance of the test reagent to the identification of the bacteria. For example, what were you able to differentiate, and how? HINT: The "Description" section of each module will help you answer this question.
For your second post, complete the "Antimicrobial Susceptibility" module. Report your findings in your post. Use the "R," "I," "S" interpretation for each bacterial isolate.
Explanation & Answer
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Running Head: MICROBIOLOGY DISCUSSION QUESTIONS
Microbiology Discussion Questions
Student’s name
Institution Affiliation
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MICROBIOLOGY DISCUSSION QUESTIONS
PART A
1. It is always necessary to flame the inoculating loop every time it is used in order to
sterilize it. This will prevent the contamination of cultures from the microbes that might
have been contained in the non-heated loop being examined in the entire procedure. High
temperatures are more likely to deactivate the functionalism of most microbes that are
sometimes present within the loop.
2. Grain staining is normally applies in the cell wall linings of a bacteria. It distinguishes the
morphological configuration of bacteria. Most of their cells react distinctively depending
on the gram composition within that particular cell. In essence, the coloration of the gram
components will depend on the existence of a layer of peptidoglycan making up the cell.
In those cells having thick layer, it will stain violet while red for those with thin linings
on their walls.
3. Agar is a typical petri dish or a plate that contains a variety of growth medium mainly
agar mixtures plus other nutrients where microorganisms like the bacteria are cultured
and observed with a magnification from a microscope. It is a general purpose medium for
nutrients that is used to support the growth and cultivation of a number of organisms.it
composes of protein enzymes, extract from yeast containing acids for the bacterial
growth, agar nutrients and mainly water components.
4. When streaking, a number of organisms involved in the process are usually reduced and
the resulting inoculation leads to the separation of a number of cells present in the
organisms. The individuals will be surfaced on the agar plate after a successive
elimination is done through the whole process. (Society for General Microbiology,
1994).
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MICROBIOLOGY DISCUSSION QUESTIONS
PART B
1. Selective media allows involves the process which allows for the growth of organisms as
opposed to the...