UF Primer that Is Well Designed Forms Most Significant Element of PCR Queries

User Generated

Xvat87

Science

University of Florida

Description

  1. What is the purpose of the final 60 minute extension for Taq polymerase’s non-template adenylation? [2]
  2. How are PCR primer’s designed? What would happen if you had a primer binding site mutation in a person’s DNA? [5]
  3. Describe three mechanisms in which inhibition can occur in a forensic sample. [3]
  4. What does multiplexing mean? [2]
  5. Why does stutter occur during amplification? [2]
  6. What type of repeat is the most common in forensic STR kits? [2]
  7. Would you expect a pentanucleotide repeat to have more or less stutter than a tetranucleotide repeat? Why? [2]

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Explanation & Answer

Attached.

Running head: DISCUSSION QUERIES

Discussion Queries
Name of the Student
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1

DISCUSSION QUERIES

2
Discussion Queries
Question #1

The final 60 minutes extension is purposed to assist in the initiating process of the
addition of untemplated products of A with its products of 3. A final rest is then provided to
assist the enzymes to complete its untemplated polyadenylation. However, when sufficient time
is not provided to add adenosine to every copy of PCR, there will be a few fragments that are one
base pair shorter compared to others. Lack of sufficient during down-streaming could generate
issues of data interpretation (Alaeddini, 2018).
Question #2
A primer that is well-designed forms the most significant element of a good PCR
reaction. Primers are synthesized chemically by the union nucleotides. Three main steps which
followed when designing PCR that includes denaturation, annealing, and extension. The first
step involves the denaturing of genetic m...


Anonymous
I was struggling with this subject, and this helped me a ton!

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