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Why do pheophytins have larger rf values than chlorophylls?

Sep 25th, 2017

"Rf values will be unique for each solvent. However, the general order of the Rf values should be the same because the more nonpolar pigments move farther in nonpolar organic solvents.
A recent plant physiology manual (Reiss 1994) identifies six pigments from spinach leaves extracted with hexane and chromatographed with petroleum ether-acetone-chloroform (3:1:1) on silica-gl chromatography. The pigments and their Rf's were:
carotene - 0.98
chlorophyll a - 0.59
chlorophyll b - 0.42
pheophytin - 0.81
xanthophyll 1 - 0.28
xanthophyll 2 - 0.15
The color of the bands can be a general guide to identify the pigments. Carotene is orange. Chorophylls are green. Chlorophyll a is a blue-green. Chlorophyll b is a yellow-green. Xanthophylls are yellow. Phaeophytin is chlorophyll lacking the central magnesium ion. Pheophytin is an olive-green."

Read more: http://www.physicsforums.com 

Oct 31st, 2014

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