UArizona Global Calculate the Limit of Detection Electrical Engineering Questions
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2. (15 pts) Figure A represents a single analytical response (H) for 0.030 mg ml mercuric ion (Hg2+). The
peak-to-peak noise (h) is estimated from the baseline below.
a. Calculate the signal-to-noise ratio of the response below at the 99% confidence level
b. Calculate the limit of detection (LOD) and the limit of quantitation (LOQ) for the mercuric ion in
units of mg mL-1 using this analytical technique.
c. Using the ensemble averaging approach, how many responses would need to be acquired to
obtain the signal-to-noise ratio shown in Figure B.
A
H
h
"
B
word
open that they had henting In
4.
(20 pts) A reputable manufacturer of packed chromatographic columns gave a research group two HPLC
columns to use, and this research group generated the two chromatograms on the following page - one
chromatogram for each column. The packing in each column is exactly the same (i.e. same article size, same
stationary phase, same inner diameter, same plate height, etc.). The only difference is their length: The column
used to generate the upper chromatogram is 100 mm long. Use a ruler or similar measurement device to
calculate the following:
a. The
The average number of theoretical plates and plate height (based on peaks 1, 2, and 3) for both
columns.
b. The plate height (assume H is the same for both columns) and the length of the column (in mm) that
generated the lower chromatogram.
c. The resolution of the critical pair for each chromatogram. Calculate the length of the column (mm) that
generated the lower chromatogram based on the difference in resolution between the two columns.
Please defend your answer.
1
2
3
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I
1
2
0
2
4
5 m
3
4
2.
7 min
0
6
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Roman font, and one-inch margins. It should be 1 to 2 pages in length and should be written in APA format. For both files, use filenames that include the course
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