Description
Please help with biochem questions for enzyme kinetics and mechanisms

Explanation & Answer

Completed , check this and ask any doubts you have
Q1) (a) Enzyme exhibits Acid catalysis and also Base catalysis.
b) This is because enzyme acted as acid (AA119) by donating H+ to substate and at same enzyme
acted as base (AA12) by abstracting H+.
c) Given pH = 7 . Given 80 % is protonated form or acidic form. This means 20 % is deprotonated
form. We use Henderson eq
pH = pka + log [deprotonated form] / [protonated form]
7 = pka + log (20/80)
7 = pka -0.6
Thus we get pka = 7.6
d) Enzyme would be most active at pH = 7 . This is because enzyme has both acidic and basic groups
present. At high pH of 9 we will have acidic group of enzyme in deprotonated form. This makes
acidic group (AA119) part inactive. Thus at pH =7 where both acidi...
