i have completed the calculation for this lab but i don;t have time to finish the writing part. the writing part is to answer 3 questions depending on the data we get from the calculation. I will upload my data exell sheet and assignment requirement.1. Which purification technique lost the most LDH? Discuss this in the context of the LDH recovery for each purification step. You should calculate the percent recovery of LDH (the fraction of the LDH that went into that step that was recovered) for each step and provide it here. Which steps recovered the LDH better, and which steps lost a lot? This should be at least 100 words but not more than 200 words.2. Which technique gave the highest degree of purification of the LDH? Tell how you are assessing purification. To which columns are you referring to determine the amount of purification achieved in each step? Specific activity is most important, but what else do you need to look at? You should discuss purification in terms of the fold-purification provided by each step. Fold-purification is the specific activity achieved by a purification step divided by the specific activity of the previous step. Be sure to answer the question, but place it in the context of the purification achieved by each technique (if you simply list numbers without placing them in context you will lose points). This should be 150 – 300 words.3. How would you modify this purification strategy to maximize the yield and purity of LDH? Which steps in the original purification strategy worked well, and which did not? What would you change to get better recovery and purification of LDH? Give each step/technique of your modified strategy and describe what is accomplished by that step. You can change the parameters or way that any individual technique is done. You can also change the order of the techniques used, remove a technique, and even add a new technique that you have Purification Intermediate Relative Activity (U/ml) Total LDH Activity Units (U) Protein Concentration (mg/ml) Total Protein (mg) Specific Activity (U/mg) Clarified Homogenate 65% Cut Pellet Affinity-Purifie d LDH LDH Final Product not performed. Just be sure that you can provide a rationale for each step in your new purification strategy based on the purification table data. How will that modification improve the purification? (Be sure you understand what is meant by this; ask if you don’t!) You should provide numbers from the purification table in making your arguments here. Keep in mind that the initial crude homogenate is not listed in the purification table, and the clarified homogenate is given as the starting material. This is because the crude homogenate is very difficult to work with; its turbidity interferes with the assays and the LDH is rapidly degraded by proteases. Even though you didn’t measure it, you may assume the initial centrifugation you performed to isolate the cellular fraction containing LDH achieved a good degree of purification from the crude homogenate. You should account for this in your new purification strategy. This should be one to two pages. Put some careful thought and consideration into answering these questions. Your score on this is based on the quality of your writing, including how well you make your arguments and provide a careful rationale for your answers.Requirements: 2 pages

Each Question should have at least a paragraph response. Just write your response under the question. NO PLAGIARISM! Cite your references if you use any.The worksheet has all the instructions on it, please follow all them. Thank you!

Assignment DetailsAntibiotic ResistanceFor many years, antibiotics have been effectively used to treat bacterial disease. A growing concern for treating bacterial diseases is the evolution of antibiotic resistance by bacterial populations. Resistance means that a particular antibiotic is no longer effective in treating a disease. This resistance can be viewed as evolution of a new trait at the population level, which is resistance to an antibiotic. Recommended: Click on the following links to review materials to increase your understanding of natural selection and antibiotic resistance:Understanding EvolutionAntibiotic ResistanceFocus your discussion on ONE of the following topics: The use, overuse, and abuse of antibiotics are accredited with creating antibiotic-resistant strains of bacteria. Explain how this relates to natural selection. Visit this tool: ResistanceOpen. Type in your location to find and report on how common antibiotic-resistant bacteria are in your area. Compare the incidence of antibiotic-resistant bacteria in your area with that in Lincoln, NE and Miami, FL. Do the results concern you? Give 1 suggestion for why there are such widely different results.Using a credible source, describe at least 2 of the things that people do (you can include individuals, doctors, health care professionals, hospitals, farmers, and so on) that contribute to this problem. Explain. Are there things that you can personally do to reduce your risk or even to reduce the spread of these dangerous microbes? Use at least 1 credible source to support the arguments presented in your post.ReferencesCenters for Disease Control and Prevention. (2018, September 10). Antibiotic / antimicrobial resistance (AR / AMR). Retrieved from https://www.cdc.gov/drugresistance/HealthMap. (2017). ResistanceOpen. Retrieved from https://resistanceopen.org/results/Understanding Genetics. University of California Museum of Paleontology. (2008). Natural selection. Retrieved from https://evolution.berkeley.edu/evolibrary/article/...In your own words, post a response to the Discussion Board, and comment on at least 2 other postings. You will be graded on both level of engagement and the quality of the contribution to the discussion.Discussion Board Reminders: Must have three posts: A Main Post and two replies to peers.First post: Either your main post or a reply to others must be posted before midnight CT (Central time) on Friday of each week.Second and third post: Must be posted on a different day from the first post.Connect to Content: At least one post must refer to course learning materials. See the Academic Resource on Discussion Boards for help with connecting to the content.Engaging in Class Discussions: For more information on making the most of your class dialog, review the Academic Resource on Discussion Boards. Grading RubricAssignment CriteriaProficient DescriptorPointsQuality of Main Post27 Points TotalStudent’s main post meets or exceeds the following requirementsResponds completely to all of parts of discussion question. (7 points)Communicates content information accurately and/or logically (7 points)Delivers a thoughtful response demonstrating insights and reflections (7 points)Connects to both key content concepts and personal experiences (6 points)/27 pts.Response to Peers24 Points Total• Responds to a minimum of 2 peers. (12 points per response)Substantive response (6 points)Furthers the conversation with peers. Examples could include: (6 points) Provides additional resourcesConnects to key conceptsShares personal or professional experiencesContributes new information or perspectivePresents a competing viewpoint/24 pts.Support from Learning Resources4 Points TotalAt least one post refers to course learning resources./4 pts.Professional Writing5 Points TotalResponse is well-organized, clear and free of grammatical and mechanical errors.Posts demonstrate courtesy and respect for others./5 pts.Total/60 pts.Assignment DetailsAntibiotic ResistanceFor many years, antibiotics have been effectively used to treat bacterial disease. A growing concern for treating bacterial diseases is the evolution of antibiotic resistance by bacterial populations. Resistance means that a particular antibiotic is no longer effective in treating a disease. This resistance can be viewed as evolution of a new trait at the population level, which is resistance to an antibiotic. Recommended: Click on the following links to review materials to increase your understanding of natural selection and antibiotic resistance:Understanding EvolutionAntibiotic ResistanceFocus your discussion on ONE of the following topics: The use, overuse, and abuse of antibiotics are accredited with creating antibiotic-resistant strains of bacteria. Explain how this relates to natural selection. Visit this tool: ResistanceOpen. Type in your location to find and report on how common antibiotic-resistant bacteria are in your area. Compare the incidence of antibiotic-resistant bacteria in your area with that in Lincoln, NE and Miami, FL. Do the results concern you? Give 1 suggestion for why there are such widely different results.Using a credible source, describe at least 2 of the things that people do (you can include individuals, doctors, health care professionals, hospitals, farmers, and so on) that contribute to this problem. Explain. Are there things that you can personally do to reduce your risk or even to reduce the spread of these dangerous microbes? Use at least 1 credible source to support the arguments presented in your post.ReferencesCenters for Disease Control and Prevention. (2018, September 10). Antibiotic / antimicrobial resistance (AR / AMR). Retrieved from https://www.cdc.gov/drugresistance/HealthMap. (2017). ResistanceOpen. Retrieved from https://resistanceopen.org/results/Understanding Genetics. University of California Museum of Paleontology. (2008). Natural selection. Retrieved from https://evolution.berkeley.edu/evolibrary/article/...In your own words, post a response to the Discussion Board, and comment on at least 2 other postings. You will be graded on both level of engagement and the quality of the contribution to the discussion.Discussion Board Reminders: Must have three posts: A Main Post and two replies to peers.First post: Either your main post or a reply to others must be posted before midnight CT (Central time) on Friday of each week.Second and third post: Must be posted on a different day from the first post.Connect to Content: At least one post must refer to course learning materials. See the Academic Resource on Discussion Boards for help with connecting to the content.Engaging in Class Discussions: For more information on making the most of your class dialog, review the Academic Resource on Discussion Boards. Grading RubricAssignment CriteriaProficient DescriptorPointsQuality of Main Post27 Points TotalStudent’s main post meets or exceeds the following requirementsResponds completely to all of parts of discussion question. (7 points)Communicates content information accurately and/or logically (7 points)Delivers a thoughtful response demonstrating insights and reflections (7 points)Connects to both key content concepts and personal experiences (6 points)/27 pts.Response to Peers24 Points Total• Responds to a minimum of 2 peers. (12 points per response)Substantive response (6 points)Furthers the conversation with peers. Examples could include: (6 points) Provides additional resourcesConnects to key conceptsShares personal or professional experiencesContributes new information or perspectivePresents a competing viewpoint/24 pts.Support from Learning Resources4 Points TotalAt least one post refers to course learning resources./4 pts.Professional Writing5 Points TotalResponse is well-organized, clear and free of grammatical and mechanical errors.Posts demonstrate courtesy and respect for others./5 pts.Total/60 pts.