Cutting and joining of DNA molecules PowerPoint, homework help

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ooo ENABLING D ol O Cutting and DNA isolati Joning of Call can DNA at re as restriction sites then specific poinb known agment that have in ". DNA use of living system of livin 1 TECHNIQUES joining of Don molecules. : be fragmented waing patien I donudeaus These a enzame that joined together using DNA Ligasas. Je cloning vectors: Canal present bren exercised endonucka maintained by replication and expressa by ligating th different types of The veders relativaly small DNA molecules that have the ability replicats readily and (independly from the chromosomas be pasant in seve several inside the host call copy The main type of vectors. gene cloning Semiche ayd buferiophages which normally used depending the size aurophages the DNA into them zase Can be w this allows numbus usad pr 右 tou vec smid Fragment that more to be ) Hast Source Anothai DNA - micro-organisa . can replicato test A. vector) Small Fragments They cules that replicate independently using y fragment อ origin The paluns their own or important or ano vectors ASR manter hosta Szter lebyla sonids as دخله The markea Carr them erry Those are the choice usa Plasmids hoe in den teint de voord in cloning small wall circular extra. chromosomal DNA replications jor plasmids as clearing Anaddition, the plasmids have contoh the resistant Markers and those are gonos that code for Potains that Confer resistance to specific antibiotice allow for the election of hata have multiple cloning sites unihe different restriction siten enabling restriction enors Jo Drop for those plasmids is navired. 4 plasmid replication in a They also determine the member of Plasmid copios found They also have inspectional maitination Marken plasmid non-recombinant cloning Large fragments DNA of 1 10 istate Coat intad dusters of gener lage formant M a. for closing of of pes The orihin Jor replicate hant which is arrant ecific so hast can Shich allows from non- - Casmids -MENA ( Go DNA MANA , C 2 gone certain of DNA For example in the responsible centelistic for Synthesis of Those an usuall Since require ا ولح am to cligerent gener H ज dan Genu an СА hoppe sing essengur Case ave home to be cloud . the synthesis of of antibiotic may cally Ranseribed present in operous RNA molecule o considerable lengte In this bacteriophage and comiets the vectors of choice The most commoditumphoge is the Escali cambda parteciphage tubular patein tail and Protein hoad Packed with approximatch those 50 kb of DNA, only then ssent for the propagation the Around 20 kb. bacteriophage. this DNA can be replaced for recombinan g they therefore very useful for the J 1 The bacterisphag complex coll cycle ayter infested into the colo multiply they enter into a lutie cycle selina the lysis the of Sabse Quent roles со a DNA of RSS 50 kb Out of half are on of \ DWA genemic libraries. generation has 1 Sina it Can toring the to to SA ot curge t 1 AID i irritatio mitentia fi mu Prict Alternatively it can into lysogenic cyclo which the phard is integrated Shena tainac It is en loring a Those can of n E Cdi essenge, une и w ning Packagin o transcend number phas particles . in E. Coli chromosom it is main until environmental conclitions, change then excised enteri Cosmids Carry up to 40 kb cloned Drit can also also be maintained in than plasmids that have been engingend with the addition of These Cosmond ends are small single slanded complementary DNA them to be packed into lambda phape particles extracts and then Once insides the bacterial host in the from the Cosmids is Circularized * the Cosmids ends after which it behans as a normal plamid Introduction host vector into a of recombinant to a expressed maintain genoctors covering them howe to be an ach and into Suitable best! Then an main methods used to achieve These A trouston Electporation Lectrofanatic E Calo DNA ecture 2 for 4 나 CO wspomaloin n- A rausmane
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ENABLING TECHNIQUES



Cutting and joining of DNA molecules.

DNA isolated from any type of cell can be fragmented using restriction endonucleases.


Cloning vectors.

Genes present in DNA fragments that have been exercised by the restriction endonucleases
can be maintained by replication and exposure by ligating them into different types of vectors.
The vectors are relatively small DNA molecules that have the ability to replicate readily and
independently from the chromosomes. This allows them to be present in several copy
numbers inside the host cell.
The main type of vectors used for gene cloning are plasmids, used depending on the size of
the DNA fragment that needs to be clone...


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