Microbe Morphology Gram St. Bacillus cereus Bacilli Bacillus pumilis Bacilli Bacillus subtilis Bacilli + + + Capsule Endospore KOH TTC Glucose Lactose Sucrose Mannitol + + + + A+/GA+/GA+/G- A-/G- A+/G- + - A-/G- A+/G- A+/GA+/GA-/G- - - - A+/G- A+/G- A+/G- - - A+/G- A+/G- A-/G- A-/G- + Nitrate Reduction - +NO2 - A+/G- +NO3 + A+/G- +NO3 A-/G- A-/G- +NO3 Enterococcus faecalis Cocci + - Enterococcus faecium Cocci + - Micrococcus luteus Cocci + - - - - A-/G- Micrococcus roseus Cocci + - - - + A+/G- A-/G- A-/G- A+/G- +NO2 Staphylococcus aureus Cocci + - - - - A+/G- A+/G- A+/G- A+/G- Cocci + + - - - A+/G- A+/G- A+/G- Cocci + - - A+/G- A+/G- A-/G- Cocci + + - - A+/G- A+/G- A+/G- Cocci + - - - A+/G- A+/G- A+/G- A+/G- A-/G- A-/G- +NO3 +NO3 Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcus agalactiae Streptococcus mitis Streptococcus mutans Streptococcus pyogenes Enterococcus hirae Cocci + - Cocci + - - - - A+/G- - - A+/G- - R - R + + + + + - S + - S - + - + - R Lipid Hydrolysis Hemolysis + Beta Beta Gelatin Hydrolysis + DNA Hydrolysis + - - Beta - + Alpha + + - S + - R A-/G- +NO3 - - - - - - - - - Gamma - A-/G- +NO3 - - - - - - - - - Alpha + - - - - - - - + Gamma + - - - - - - - - Beta + + + + + - R + + + - R A-/G- Lactobacillus plantarum Bacilli + - - - Bacilli + - - - Bacilli + + - + - + A+/G- + R - - + - + - - + + - + R - S + - + Beta + Urease - - - + + Gmma - + Gamma - + - - Alpha - A+/G- - + A-/G- - A-/GA-/G- S - + + - + R Starch Hydrolysis - A-/G- - - Coagulase + - A-/G- V - Casein Hydrolysis - A+/G- A+/G- - + MSA - A+/G- A-/G- + + PYR + A+/G- A+/G- - + Lysine + - + - + Bacitracin + - - Erythromycin +NO3 - - Novobiocin +NO2 A+/G- A+/G- - A-/G- - + - Oxidase + + + A+/G- - + + Catalase - - + + NaCl - + Bacilli + Bile Esculin + + Bacillus megaterium + +NO2 Cocci Cocci Citrate - - Cocci Lactococcus lactis - VogesProskaur - Enterococcus durans Corynebacterium xerosis Bacillius coagulans Methyl Red +NO2 + +NO2 - - + + A gray box means this test is traditionally not performed for this organism. Stands for varies. You may receive positive or negative results. Take these results essentially "with a grain of salt" - - + - + Alpha or Gamma Gamma - Student Name: Introduction Concise, descriptive title (0.5 pt) Background of project, in your own words. (4 pts) Broken into weekly paragraphs (1 pt) Summary of project (4 pts) Weekly paragraphs include background explanation of tests received, with any additional reagents, indicators, etc explained. (20 pts) Title page formatted correctly (1 pt) Subheadings present (0.5 pt) Total Grand total Biology 2420 Unknowns Report Rubric - Last Updated Spring 2021 Title Page ___ out of 2 pts Unknown # listed (1 pt) ___ out of 9 pts Methods Results Conclusion Observations of test results described. (15 pts) Summary of tests (10 pts) Description of result interpretations. (15 pts) Statement of suspected unknown (1 pt) Unknown Correct unknown ID (5 pts) Explanation or background of bacteria identified (1-2) sentences. (3 pts) Weekly paragraphs include list of tests requested. (2 pts) *Must include all tests provided & requested. -2 pts for from grand total for every missing test. *Must include all tests provided & requested. -2 pts for every missing test from grand total. ___ out of 23 pts ___ out of 30 pts ___ out of 100 pts Bibilography Page Minimum of 2 sources Correct gram nature / (lab manual must be morphology ID (5 pts) one) (2 pts) Grammar / Syntax Correct use of grammar (2 pts) In-text citations in introduction and methods formatted correctly (2 pts) Paper is written in past tense, third person (2 pts) Bibilography page formatted correctly (2 pts) Proper sentence structure used throughout paper. (2 pts) *Papers with no in-text citations in the introduction or methods will receive a zero for those sections. Papers with direct quotes in the introduction or methods will receive a zero for those sections. ___ 14' pts ___ out of 10 pts ___ out of 6 pts ___ out of 6 pts KOH TTC EMB MAC Glucose Lactose Sucrose Mannitol Nitrate Reduction S/I/M Methyl Red VogesProskaur Citrate + - Light purple Hot pink A+/G+ A+/G- A+/G- A+/G- +NO2 +/-/- + - + + Light purple to pink Hot pink A+/G+ A+/G- A+/G- A+/G- +NO2 +/-/+ - - + - Colorless A+/G- A-/G- A-/G- A+/G- +NO2 -/+/- Bacilli - + A+/G- A+/G- A+/G- +NO2 -/-/V Alcaligenes eutrophus Bacilli - + Escherichia coli Bacilli - + Klebsiella pneumoniae Bacilli - Pseudomonas fluorescens Bacilli - Morganella morganii Bacilli - Citrobacter koseri Coccobacilli - + + Proteus mirabilis Bacilli - + + Yersenia fredericksenii Bacilli - + - Proteus vulgaris Bacilli - - + + Pseudomonas aeruginosa Coccobacilli - - + + + + + + Microbe Morphology Gram St. Citrobacter freundii Coccobacilli - Enterobacter aerogenes Bacilli - Shigella flexneri Bacilli Enterobacter cloacae Salmonella typhimurium Bacilli - Pantoae agglomerans Bacilli - Serratia marcescens Alcaligenes faecalis Coccobacilli Coccobacilli - Capsule + + - - + + - + - + + + DNA Hydrolysis Urease A/A/G - - - - + A/A/G - - - - + - K/A - - + + - A/A - - - + + + + A/A/G + A/A/G Lysine TSI + + - + - - + + + - + + + - - - - + - + + + - + - - - + - + + + + + - A+/G+ A-/G- A-/G- A-/G- A-/G- +NO2 -/-/+ - - + Metallic green Hot pink A+/G+ A+/G- A-/G- A+/G- +NO2 -/+/V + - - Light purple to pink Hot pink A+/G+ A+/G- A+/G- A+/G- +NO2 -/-/- + - - A-/G- A-/G- A-/G- A-/G- +NO2 -/-/+ - - + Colorless to lavender Colorless, transparent, yellow A+/G+ A-/G- A-/G- A-/G- +NO2 V/+/V + - - A+/G- A-/G- A-/G- A+/G- +NO2 -/+/+ + - + - + - Colorless to lavender Colorless, transparent, yellow A+/G+ A-/G- A-/G- A-/G- +NO2 +/-/+ + - - - + + A+/G- A+/G- A+/G- A+/G- +NO2 -/+/- + - - Colorless to lavender Colorless, transparent, yellow A+/G+ A-/G- A+/G- A-/G- +NO2 +/+/+ + - - Shiny to greenish Shiny to greenish A-/G- A-/G- A-/G- A-/G- +N2 -/-/+ - - + Colorless to lavender Colorless, transparent, yellow A+/G+ A-/G- A-/G- A+/G- +N2 +/-/+ + - + + A+/G- A-/G- A-/G- A+/G- +NO2 -/-/+ + - + - - + + Light purple to irridescent Hot pink A+/G+ A-/G- A+/G- A+/G- +NO2 -/-/+ - + + + Colorless, translucent Colorless, transparent, yellow K A-/G- A-/G- A-/G- +NO3 -/-/V - - + - V Gelatin Hydrolysis Phenylalanine Hot pink Colorless Lipid Hydrolysis Ornithine Light purple to pink + + Bile Esculin A gray box means this test is traditionally not performed for this organism. Stands for varies. You may receive positive or negative results. Take these results essentially "with a grain of salt" + - - - + + Catalase Oxidase - - - - - + + K/A/G - K/A/H2S - - - - + - + - + + + - + - - + - - A/A/G/H2S - - + + + K/K + - + K/A/H2S + + - A/A - + K/A/G + + + K/- + - + + - Casein Hydrolysis + - - - + - - - + - - - 7:00 X Spring 2021 Unknowns ABC... 4 Mix And lift Gram Stain Vancomycin KOH