writing summary for research paper.

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I want to do summary for research paper. Also, this summary require something that i will download by field for you. explaining each graph or image in research paper , please. Thank you

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Glycoconj J (2013) 30:857–870 DOI 10.1007/s10719-013-9490-x Chemoenzymatic synthesis of immunogenic meningococcal group C polysialic acid-tetanus Hc fragment glycoconjugates Pumtiwitt C. McCarthy & Rina Saksena & Dwight C. Peterson & Che-Hung Lee & Yanming An & John F. Cipollo & Willie F. Vann Received: 28 March 2013 / Revised: 25 July 2013 / Accepted: 28 July 2013 / Published online: 16 August 2013 # Springer Science+Business Media New York (outside the USA) 2013 Abstract Vaccination with meningococcal glycoconjugate vaccines has decreased the incidence of invasive meningitis worldwide. These vaccines contain purified capsular polysaccharides attached to a carrier protein. Because of derivatization chemistries used in the process, conjugation of polysaccharide to protein often results in heterogeneous mixtures. Well-defined vaccines are needed to determine the relationship between vaccine structure and generated immune response. Here, we describe efforts to produce well-defined vaccine candidates by chemoenzymatic synthesis. Chemically synthesized lactosides were substrates for recombinant sialyltransferase enzymes from Camplyobacter jejuni and Neisseria meningitidis serogroup C. These resulting oligosialic acids have the same α(2-9) sialic acid repeat structure as Neisseria polysaccharide capsule with the addition of a conjugatable azide aglycon. The degree of polymerization (DP) of carbohydrate products was controlled by inclusion of the inhibitor CMP-9-deoxy-NeuNAc. Polymers with estimated DP<47 (median DP 25) and DP<100 (median DP 51) were produced. The receptor binding domain of the tetanus toxin protein (TetHc) was coupled as a carrier to the enzymatically synthesized oligosialic acids. Recombinant TetHc was derivatized with an alkyne squarate. Protein modification sites were determined by trypsin proteolysis followed by LC/MS-MSE analysis of peptides. Oligosialic acid azides were conjugated to modified TetHc via click chemistry. These chemoenzymatically prepared glycoconjugates were reactive in immunoassays with specific antibodies against either group C polysaccharide or TetHc. Sera of mice immunized with oligosialic Electronic supplementary material The online version of this article (doi:10.1007/s10719-013-9490-x) contains supplementary material, which is available to authorized users. P. C. McCarthy : R. Saksena : D. C. Peterson : C. Purchase answer to see full attachment

Final Answer

please find the work...


The main objective of this article is to produce vaccines that are well-defined to enhance
a better understanding of how glycoconjugate vaccine structure relates to the immune response
generated. In order to actualize this, the Author sought to modulate in vitro incubation with the
Neisseria CMP-NeuNAc synthetase by conducting Small scale reactions meant to reveal the
effects of these inhibitors on oligosialic acid production. Further, the article is centered on
explaining the causative agents of meningitis and the method that has been deemed effective in
the management of the condition on a global perspe...

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