-pH- Enzymes function best at a pH of 7.4, and if the pH is too high or the enzyme is denatured and doesn't function anymore
-Temperature- To a certain extent, the higher a temperature is the
faster the enzymes works because the substrates are moving faster (an
enzyme finding a substrate to bind to is the "slow step" of an enzyme
catalizing a reaction) However, if the temperature is too high or low,
the enzyme will be denatured and not function anymore.
- The amount of inhibitors around- Inhibitors stop enzymes from
functioning: there are two types of inhibitors, competitive inhibitors
which have a similar shape to the substrate and therefore can "compete"
with enzyme. There is also noncompetitive inhibitors, which do not bind
to the active site but block the enzyme substrate complex from forming.
They react with portions of the action site, changing the enzymes shape
so it does not function anymore