DNA Function Question 1 Select one answer. During DNA replication, it is possible for the T residue in the template strand to form two hydrogen bonds with a T base on the new strand. This doesn’t normally occur because: A. The T-T basepair contains two of the smaller sized bases. B. The two T-T hydrogen bonds will be weaker than the two hydrogen bonds between an A-T pair. C. DNA polymerase cannot incorporate T into the newly synthesized strands. D. The normal A-T base pairing uses 3 hydrogen bonds, so it will be preferred. Question 2 Select one answer. If radioactive nucleotide bases were provided to bacteria during DNA synthesis, then after cell division you would find: A. Neither would be radioactive because DNA polymerase can distinguish between isotopes. B. One bacteria would be radioactive and the other not. C. Both bacteria would be equally radioactive. Question 3 Select one answer. PCR differs from DNA replication in which of the following ways? A. A polymerase is needed. B. Double stranded DNA is the product. C. A single copy of DNA is made. D. Primers are needed. Question 4 Select one answer. A typical PCR cycle consists of steps at different temperatures, typically 55, 78 and 98 oC. The correct order of these steps is? A. 78, 55, 98 B. 98, 55, 78 C. 98, 78, 55 D. 55, 98, 78 E. 55, 78, 98 F. 78, 98, 55 Question 5 Select all that apply. In which of the following organisms is it necessary to transport the mRNA across a membrane prior to protein synthesis. Select all that apply. A. Animals B. Plants C. Bacteria D. Viruses Question 6 Select one answer. Does the DNA in the skin cell have the same sequence of bases as the DNA in the brain cell of the same organism? A. No, some cells will have mom’s DNA, other cells will have dad’s DNA. B. Yes, the sequence of ALL DNA is the same. C. Yes, the sequence of bases should be the same in all cells of an organism. D. No, the DNA sequence will vary based on cell type. Question 7 Select one answer. What is the end result of DNA replication? A. Two DNA molecules which each have one old strand and one new strand. B. Two DNA molecules each both have two new strands. C. Two DNA molecules one with two old strands and one with two new strands. D. Two DNA molecules which each have two new strands and two old strands. Question 8 Select one answer. Which part of the nucleotide stores the genetic information? A. Nitrogen base B. Ribose C. Phosphate group D. Deoxyribose Question 9 Select one answer. What process is responsible creating distinct sets of proteins in skin cell compared to brain cell? A. DNA Mutation B. DNA Replication C. Gene Expression D. Base Titration Question 10 Select one answer. Which of the following statements is FALSE? A. Both DNA replication and PCR use primers. B. Both DNA replication and PCR use DNA polymerase. C. Both DNA replication and PCR use a DNA template. D. Both DNA replication and PCR use helicase.
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