College of DuPage Why GFP Protein Fluoresces Green Discussion Questions

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College of DuPage

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  1. 1. How (what is the procedure) do you make a cell that does not normally express a fluorescent protein like GFP, express a fluorescently tagged endogenous protein (like tubulin) and glow green? I want some detail in this answer. You can use an image or diagram but explain what you are showing me. Cite your source
    2. Why is it that GFP fluoresces green?

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Explanation & Answer

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1

Biology

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2
The procedure of Making a Cell that Express a Fluorescently Tagged Endogenous Protein
(like tubulin) and Glow Green?
Making cells that express a fluorescent-tagged endogenous protein like tubulin entails the
cloning process and insertion within the codons. Tubulin is tagged with a green fluorescent
protein photoactive variant (PA-GFP). The tracking and expression activity of the recombinant
cell...


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