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Running head: MICROBIOLOGY CONCEPTS
Using light microscopy, you examine the soil samples and the 'goo' from the degraded
polyurethane. Will this approach allow you to observe all microorganisms present in the
Why or why not?
What are the limitations of this approach?
Light microscopy usually enables us to see we would not be able to see with our own unaided
eyes. For example, the compound light microscope has different lenses that can refract light
differently to see organisms at different magnifications (lab 2 protocol). The light microscope
can allow us to see organisms as small as 200 nm (lecture 3 slides). However, this type of
microscopy does have its limitations. With bright field microscopy, you can only see dead,
stained organisms that can be seen in contrast with the light background (lab 2 protocol). Hence,
microorganisms in the soil samples and goo that are even smaller than 200 nm, colorless, alive
and do not absorb and reflect light cannot be seen with this type of microscopy.
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