UMUC BIOT630 Lecture 9 Exercise – “Due Version” Question 1.a Are there any Evolutionary Conserved Regions (ECRs) between the genomic sequences for Mouse FASL, Rat FASL, and Human FASL? To answer, use the sequences found at the end of the Exercise in conjunction with “multi-zPicture” (https://zpicture.dcode.org/multiz.php) with the Mouse sequence entered as your reference (i.e., “SEQUENCE 1”), the Rat sequence entered as “SEQUENCE 2”, and the Human sequence entered as “SEQUENCE 3”. Copy/paste the resulting “Dynamic Visualization” as your answer. Answer: ? Question 1.b How many ECRs exist between Rat FASL and Mouse FASL (Hint: click on list of ECRs for “seq 2” in the Output files results section)? Answer: ? Question 1.c How many ECRs exist between Human FASL and Mouse FASL (Hint: click on list of ECRs for “seq 3” in the Output files results section)? Answer: ? Question 2. What are the Evolutionary Conserved Regions (ECRs) when “mVISTA” is used? To answer, save the sequences found at the end of the Exercise to individual “.txt” files. After doing so, upload the files into mVISTA and click on “Submit”. After, copy/paste the “PDF version” of the “VISTA Image” with the Mouse sequence represented as the reference. mVISTA url = http://genome.lbl.gov/vista/mvista/submit.shtml Answer: ? Question 3. What are the Evolutionary Conserved Regions (ECRs) between Mouse and Rat when the “UCSC Genome Search” tool is used? To answer, paste in the Mouse sequence found at the end of the Exercise into the sequence window of the tool, select the “Rat” genome in the “Genome” drop down menu, then click “Submit”. On the return page, click on the “detailed” hyperlinked text for the top hit. On the return page, scroll down and take a screen shot of the top portion of the “Genomic” results section and provide as your answer. url = https://genome.ucsc.edu/cgi-bin/hgBlat?command=start Answer: ? Question 4. What are the Evolutionary Conserved Regions (ECRs) between Mouse and Human when the “UCSC Genome Search” tool is used? To answer, paste in the Mouse sequence found at the end of the Exercise into the sequence window of the tool, select the “Human” genome in the “Genome” drop down menu, then click “Submit”. On the return page, click on the “detailed” hyperlinked text for the top hit. On the return page, scroll down and take a screen shot of the top portion of the “Genomic” results section and provide as your answer. url = https://genome.ucsc.edu/cgi-bin/hgBlat?command=start Answer: ? Question 5.a What does the conservation across species for Mouse, Rat, and Human “FASLG” (Tumor necrosis factor ligand superfamily member 6, RefSeq version) look like using the “ECRs browser” when Human is used as the “Base” genome? To answer, copy/paste the browser view depicting Mouse and Rat against Human as the reference genome. ECR Browser = https://ecrbrowser.dcode.org Answer: ? Question 5.b What are the Evolutionary Conserved Regions (ECRs) based on your answer to Question 5.a? To answer, click on the linked option “ECR” located at the top of the browser page. Copy/paste screen shot of the ECRs as your answer. Answer: ? Sequences to use for Exercise: >Mouse tgaggcttctcagcttcagatgcaagtgagtgggtgtctcacagagaagc aaagagaagagaacaggagaaaggtgtttcccttgactgcggaaacttta taaagaaaacttagcttctctggagcagtcagcgtcagagttctgtcctt gacacctgagtctcctccacaaggctgtgagaaggaaaccctttcctggg gctgggtgccatgcagcagcccatgaattacccatgtccccagatcttct gggtagacagcagtgccacttcatcttgggctcctccagggtcagttttt ccctgtccatcttgtgggcctagagggccggaccaaaggagaccgccacc tccaccaccacctgtgtcaccactaccaccgccatcacaaccactcccac tgccgccactgacccctctaaagaagaaggaccacaacacaaatctgtgg ctaccggtggtatttttcatggttctggtggctctggttggaatgggatt aggaatgtatcagctcttccacctgcagaaggaactggcagaactccgtg aggtaagcctagcacatggattagcagccctcgcgttcctggctgcaacc tgactctgctgcgtgggagtagggacttgctatggcccatgtgggctact gtgttgcttgcaatgatttggggttgttgttgttgttgttgtttttaaag gcaaggatcttttcaatcccctctaatttagaaaagagttcttagagtct ctttagagttggagaatcaggcgcacacgccctcaaagagagttgttctc aacctgtgggttgcgaccccttagtgaggggagtcaaaccactattttac gggggttacataccagatattcggcatctcagatatttacattatgggtt tgtaactgtagcaaagtttcagttatgaagtaaccatgaaatcattttat ggttgggggttgccataaccttaaggatgatgcagcgttagaaatgttga gaaccactgctcacaggaagttattttccatcttctagagaattcaaggc aaatacttattcctctctgagttgtcaagggtgtttgatgagcttctcgc tgcttcatgtttagtgattcaaggatgaaaatcaattttgtgtcttgttc agaagtgtaaataaattaattttcttcaaagagacttagaagtcttacta ctactctagatacaattgtgtggctaaaccctaactcgggccaggtctga catatctgagtcctctttctatggattgctgtgacttctgcttccttggc ttaaataactgcacatttattctatgactctctttctctcactagttcac caaccaaagccttaaagtatcatcttttgaaaagcaaataggtaagtact tttatgtctgccttgtgagttcccatagctggtcttcaacactcaataaa gttgggaggatgcctaggattctgtgcctttcttgggcttatgtttccta ggcatgaaaccctgaggagcttttgactcttcgttcagtgtggttctgtg aacgatgtgaaaatattgactgtagcttattgctcaatatcagtgtatct gaatgaacactgcccacaactcaaacgaatttggacttgactttggaaag agacacacatatagccctggaccttctcccttggctccttccaagggtat cttggtcccatcttacgttgtggtggctgtaactcctagggacagaaggg gagtagaggcaaaaatactagatgtaaaatactagaccaattaggttcag tgcccaagcctcctgtctgaaactgggactaggagaacagtcccccagtg ccttgttacctattaggactatacaatgcatagagaaacttccacctctt agtcgtggcccagaaatgagaagcaaatacaaagctggtttcctttgtaa gggcaacatgacaagtgtattctaataactttgaagaaaaccaaaccatc cataaaaaggggatggatccaccacagtgcagtttggtctacatgtgagt ccctcaacaactggagagggggttgtccctgaatcttttgccttcctgta gatcccattcccctaattgggctgccttgtctggtctcagtgggagagta tgtgtctagtcctgagtgacttgatgtgctagagtggattgatacagggt gtagcagaggctcccttctcaaaggagaagaggagggggagggactgtgt gaggggaggataggggagggactgcaatctgacgtaaattgaataaatta attaatggaaaggaagggaggaaggaaggaaggaaggaaggaaggaagga agacagacagtgaccagtcagctcctgagtgaggatataagctctttcag gggtcaccatggtgtgctctgattttcaactctgcattcaaaggaccaga gcccaggatacacaggcacagagtaaataacttgttagaaaaacaaaacc tctcaggatttctaaagtgaaatcatttttgaaatcagtggagcatggct tgtgaaacagatggtgatcacccatgttcacccactcgatggaatcgttt tagtgaatagaagtcaaatgaacagatgagtcaggtcgcctgccccagtg cttatcttctttataccaacgtagatggccagaaattgataaaccatcac aaaagtatcaaaatcgcaactttactgtcaagaggagctgatttaaagaa aggaatatttatttcttccatcaaattcataaaaaggaaaaaaaacccaa agattatcaaatatgggaggaaaaaaagccatatttggcacaagtttcat ccaatgtgcatggaaattgctgttaagaatcctgaagaacactgggaaag ttcattccaaggtcttgaactgttagtctaccttgtaattacatcaccaa aggattcttttagttaagagtacatatatgtgtgtgtgtgtagtgttcat cactttcttataaaacaacaacagtttgaacagtttggaagaaactgaac tctcacaatagaagatgagttgaaatacaataggggaacatcatggcaac caaaaccaccttgtgggacttttaatgccacgaatattgcgtacttaatg ctgttaggtaaacagacaagttatcaaaggccattttcagtttaataata aaattgcatgtgcgtgtttttaaacttctaagaaaatactttttcaaaca tttatttattttattgtgtgtttatgtacacgtgcacatgtgtgtgccag agcttgtgcgtagaggtcagagaacaacctgtgggaataagatctctcct tctacctgtgggttccagggatcaaactcaggtgctcaggctcggcagca agtgccttttctcactcggccgtctgcccggtcctatttctttttacact gctaccttccccatagttagctaactggacatcatatttatatagcaggg ctttagagagcctcccatgacagaggcttagtcttggttggtgattgagg atgagaaagaagggtggatgctttttctcagcaagctgatagcacagtgg agaagaggaacaaaaacaaacccacaacacagtttcaatctgtactaaga atgatacaaagcagagtggtctggaaaagtcctttatagggtggtttgcg ctaatgcacaagggatcagccagacatgtcttggagagaagaggtgtgta tgggtcagagagcaggtaggacattctccatttcacatgcaaggagcctg aggcaggaaaagatcttttcttgtatttctggatattgttagaacctctc gagcagatacttataggagtatgttttatgagaataacataatactagac tgtatgaggaatataaaggtaagcaatgtgtggttctctgctccatttct tgttcgtgtgtggcagaatgccaggttagctgctcaggtggtagtcttgc ctatcctgctcctcacttttttcaagggggcttgtcccaggtgctagaga ggacgcgagaaaggacacgattgtttctagggtgatctcaatagaatggg ctattttctcacccagggtgtgttttatagtctacttccaagcctgaggt ggacaagtaccttcctataaatatttttttaatgtatcaattagctgaat tcatgaagcccaataatagatttctaccttagctgaaatatttgaagtta gagaaacagtgtttatttttaagaatattatcaatgttaactatgtcaat ttaatttattggatgctgtgctaaaccagactaaatgctctttgtgactt taccatattgtcaaatgccactagggaaagacttggtggccaggttctca aagcccagcagtgttagtactggtttagtcataaagtgtttattctagaa aggtacagttttcctgaacaagaagaaagcaaagatatatatatatgtgt gtgtgtgtgtgtgtatgtgtgtgtgtatgtgtgtgtgtgtatatatatat gtgtgtatacacacacacacacatacacacacacacacacacacacacaa atatatatatatatatatatatatatatatatatttgccccagcaaggag aaggcagaagtatcaagaaaggaaatgacagagggattttaggtttcaaa ggttgaagggtcatagttgacaaaaggtgctgaagggtttcccaaaggaa gagcagtgaagatggtgttcagagaattgacaggaatttaacaccgggat ctcatctggagggtgttacagacagaatgaggaaaggcccagggacaggg aagaaggggcataactaggtcataggactcctgctgtgtgcaagtgctca gtgtgatggaggaggagaggtaaggcaaaggcaacctgatgaactaaaaa tctgggcaacctcatgacctgtgaggaggccttaatagagtcagtttggg aatagactgtaggatgataaccagaaggtgaagcagtcatcttttctaga ctctcatcagccagcagaatcaggataacagtcagccttggtgcacattg gttgcttccatcactatggtcaatcagcttctaatatctttttgcagatc atatatatttaggggagtaattttgacctaaaggggccaaggcaagttct aaatggaataacagaaatagcagtcttgtgcagcaatgtttgtattcact gttttattgcaatgatatatatatatctttccctatcttgtctttttaaa ctgtttttgaaatacacacttctatgagtcccccagtttagattttctcc atgagtatgtactagaatatttcatcttatgctctgaaaatcttatattt aaatgatgggatttagagtcctatcgagatatatcatagtctgcctttat cataataattttcacatgtcttttatctctctgttatgcagccaacccca gtacaccctctgaaaaaaaagagccgaggagtgtggcccatttaacaggt ttgtgtctggaaggagtgggtggggacagtaagcctttggaaagctgtgg accaactgctaaatttccattgcagctcttggaaagtattactgacaact gtagttatcactcaagaccatccttatctcttggatctcatgaccctagt ctgacagaacttaatgctatggaattatgtatgatttggtcttgcccatg caaagcatactcttttcaaccattccaaaatctgcagggggatttgtgac ataggttcaacaggaggagcaccagagggtgaaatggtgaactattttga gtaagaagagagttctggcatttgtgggctttcagactaagattataaag tgaagaagtggtttggtgtaatgtggtatgagcacctccagtagaaaaat aaacataaaaaatgtggataaatatcacactaagaggtctaccagctcct gtaatcacatggagactgtagacaagttcatctggatccacaaacattta ttgagtacttgctgtgtgtaggtacatttaatactatgtgaatacaaagg aaggagattagcccaaaagaagaggtcaaggaatccaaactaaatgagga gatggaccacataatcatgagactttgtccagatgttgaactccagagat ccccttttttatggaagagtccccaagcccagaagtgtaggaactagttt aaattataaagtgtttattacagagatctgaaattatcctcataatcttc cccgagtttcacctgaggcacatactaactttgtcccatttatatggatg attaaaatgaggtggggaatttggcaaacgtgctgaagaaactcagtggg ggaccatgttttacctcctcagccactttttcatcagaagtcattctgta tagaactttagttaagggatgcatgtcaggtgtgtgagcctttctgcctg aacgttcctctctggtctattacagggaacccccactcaaggtccatccc tctggaatgggaagacacatatggaaccgctctgatctctggagtgaagt ataagaaaggtggccttgtgatcaacgaaactgggttgtacttcgtgtat tccaaagtatacttccggggtcagtcttgcaacaaccagcccctaaacca caaggtctatatgaggaactctaagtatcctgaggatctggtgctaatgg aggagaagaggttgaactactgcactactggacagatatgggcccacagc agctacctgggggcagtattcaatcttaccagtgctgaccatttatatgt caacatatctcaactctctctgatcaattttgaggaatctaagacctttt tcggcttgtataagctttaaaagaaaaagcattttaaaatgatctactat tctttatcatgggcaccaggaatattgtcttgaatgagagtcttcttaag acctattgagattaattaagactacatgagccacaaagacctcatgaccg caaggtccaacaggtcagctatccttcattttctcgaggtccatggagtg gtccttaatgcctgcatcatgagccagatggaaggaggtctgtgactgag ggacataaagctttgggctgctgtgtgacaatgcagaggcacagagaaag aactgtctgatgttaaatggccaagagaattttaaccattgaagaagaca cctttacactcacttccagggtgggtctacttactacctcacagaggccg tttttgagacatagttgtggtatgaatatacaagggtgagaaaggaggct catttgactgataagctagagactgaaaaaaagacagtgtctcattggca ccatctttactgttacctaatgttttctgagccgacctttgatcctaacg gagaagtaagagggatgtttgaggcacaaatcattctctacatagcatgc atacctccagtgcaatgatgtctgtgtgtttgtatgtatgagagcaaaca gattctaaggagtcatataaataaaatatgtacattatggagtacatatt agaaacctgttacatttgatgctagatatctgaatgtttcttggcaataa actctaatagtcttcaaaatcttttattatcagctactgatgctgttttt ctttaatacaactagtatttatgctctgaacatcctaatgaggaaaagac aaataaaattatgttatagaatacagaaatgccttaaggacatagacttt ggaaatc >Rat tcagagtcctgtccttgacacttcagtctccacaagactgagaggaggaa accctttcctggggctgggtgccatgcagcagcccgtgaattacccatgt ccccagatctactgggtagacagcagtgccacttctccttgggctcctcc agggtcagttttttcttgtccatcctctgggcctagagggccaggacaaa ggagaccaccgcctccaccaccacctccatcaccactaccaccgccttcc caaccacccccgctgcctccactaagccctctaaagaagaaggacaacat agagctgtggctaccggtgatatttttcatggtgctggtggctctggttg gaatggggttaggaatgtatcaactctttcatctacagaaggaactggca gaactccgtgaggtaagtctagcacatgggttaataggcccttgagttcc tggactctgctgtgtgggagtagggacttgctatggcccatgtgggccac tgtgttgtttgcaatgatttgaggtttttgctgttgttttgttttgaatg gcaaagatcttattgatcccctcgcttttagaaaagagttgttatagtct cttctttctagttggagaagcaggcgttcactccctcaaagagagtgatc ctcactcaacctgtgggtcatgaccccttagtgggggagccattttatgg ctgggggtcaccacaacatgaggaactgtattaaagagctgcggtgttcc cttgagaaccactgctcaaaggaagttattttccaccttctagacaattc gaagcaaataattattcttctctcacttgtcaatggtgtttgatgaactt tttattgcttcatatttagtgattcaaggaagaagataaactttaaatct tgttcagaagtgtaaataaatctctttgaaaagacttagaagccttacta taactctagatacaattgcatggccaaactctaactcagtcagggtctga catatttgagtcctctttctatggactgctgtgacttctgcctccttggc ttaaataactccacatttattctatgactcttttttcccccccactagtt caccaaccacagccttagagtatcatcttttgaaaagcaaataggtaagt acttttatgtctgtcttttgagttcccggaggaactcaacactcaacaaa gttgtgaggatgcttgagattccgtcccattcttgggctcatgattccta ggcacaaaatcctgaggaacttttaactctttgttcagtgtgaaaaggta gtttactgctcaatatcagtgtatctgaatgaatatcgaacgtaactcaa tagaatttggacttgactggaaaggagcacacatacagccctggatcttt tcccttggctccttccatggatatcttggtcccatcttacattgtggtga ctgtgactcctaaggacagaggcaaaaatactagatgcacaatattagac caattaggttcaatgcccagcttccctggtatgaaactggggctaagaga acagtcccctgacgccttgctatctattaagactttacaacgcacagaga aactcccacctttcaataacggctgagaaatgagaagcaaatgcaaagct ggtgtctcttgcaagggcaacacaacagttttatcctaataactgaagaa aaccgaaccttccatcaaaaggagtgctggctccacactcagtgcggctt ggtcttcatgtgggtccctcaacaactggagtgggggctgtccctgaatc tgttgccttcctgtggatcccactcccctaactgggctgccatgtctggc ctcagtgggagaggatgtgcctaatcctgagtgacttgatgtgccagagt ggattgatatcggggggggggggatgggggtgggggtcgctcccgcttct caaaggagaagaggaggggtaggaggagggactgtgtgagggaggatggg ggagtggctgcaacccggatgtaaagtgaataaataaattaattaatgga aaggaaggaaggaaagaaagaaggaagaaagaaagggaggaagggagagg gagacggagagggagagagagagggagagagagagacagacagagagaga caggaaggaaggaaggaaggaaggaaggaaggaaggaaggaaggaagggg atataagctctttcaggggtcactatggtgtgctctggttttcagctctg cattcaagtctctaaggaccagagcccagtttacacaggcacagtggaaa atgacttgttagaaaaatgaaacttctcaggatttctgaaatgaaatcat tttgaaaatcagtggagcgtggcttatgaaacagatggcactcactcatt ttcacccactcgatggaatcattttagtgaagagaagtcaaatgaacaga tgagtcaggtcgcctacccagtggttatcttcagaaattgataaaccatc acaagaacgtcaaaaattgaaactttactattaagaggagcttatttaaa aaaaggagtatttatttcttcaatcaaattcataaaaagaaaaaaacccc aaagattatcaaatgagagagagagagagagagagagagagagagagaga gagagagaggaaaagccacgtttggcacaagtttcatccagcatgcatgg agattgctgtaagaattctgaagaacactaagaaagttcataccgaggtc ttgaactgtcagtctaccttgtcatcgcatcaccgaagggttcttttggc tgggagtgttcatcgctttctgataaaacgacaaccgtttggaagaaacc gaactctcagagtagaagatgagttgaatacacttcggcacatcaatagg ggaacatcacgaacagcaacccaaaccaccttgtgagacttttaatgcca tgaatagcatattcttcatactgttaggtaaacagacaagttatcaaagg ccattttcagtttaatactcattatgaaagtatgcatgcatgtttttaaa cttctaagaaaatactttttctaaacattttttttcttgtgtgtttacat acacaagcacacatgtataacttgtgggaataagttctctccttctccct gtgggttccagggatcacactcaggagctccgactcggcagcaagtgcct tttctcacgcagcagccatctttttatactgttaccttccccgtggttag ctaactggacatcttatgtatctacaaggtttttagagagcctcctatga cagaggctgagtcttggtgattgaggatgagagagaaagacggatgcctt ttctcagcaagcacagtagagaagaggaacaaacacgaaccaacaataca attccaatccgtactaagaaggatgcagagtagagcggtctgggaaagtc ctttatagagtggtttgtgctgatgcacaagggatcaatcagacccctct tctcttggagggaagaggtgtgcatggtcagggagcagagaggacattcc acatttcacatgtaaggagcctgaggcaggaaaagtctttccttgtattt ctggatattgctagaacctctccggcagatatttatgggagtatatttta tgaaagtaacattatactagattgtatgaagaatttaaagataagcagcg tgtggttcccagcttcatttcctgttagtgtgtggcaggttagctgttca ggtggtttgtggtgtataaaaggtctagtcttgcctatcctgttcctccc tttttttcaaggggggctggttctaggtgctagagaggacacagttgttt ctatgatgatctaaatagaccgggctattttcacactcagtgtgtgtttc atagtctacttccaaacctgaggtggacaagtaccttcctataaatattt tttaatgtatcagttagctgaattcatgaagcccagtacgagagttctac cttagctgaagtatttgaagttagagaaatagtgttcatttttaagaata ttataaattttaattacatcaatttaatttactaatgctatgctaaacaa ggctaaatgctcttgtgactttaccatattgtaaaataccagtagggaaa gacttggtggccaggctctcaaagcctagcagtgttagtactagtttagt cataaagtgtttattgcagaaaggtgcagttttcctcgacaagaaggaag gaaagacaagttttttataattgcctcagtaaggagaagccagaagtatt aagaaaggaaaaaacagagggatttttggtttcgaaggttgaacggtcat agttgacaaaagagcagtgaagatcgtcattagagaattgacgggaattt aaccctgagatctcatctggagggtgttgcagacagaatgagcaaaggcc caggggcagggaagaaaggacataatcaggtcacaggactcctgctgtgg gcaagcactgggtgtgatgacggaagggaagtgaggcaaaggcaacctgg acaacctcatgatctgtgaggaggtcttaatagagtccatgtggggatag atgtaggctgatggtccggaggggaatcagtagtgttttctagactcgta tcagtcagcagaatcaggggatacagaaccccagattaacagcctgggtg cacattgattgcctccatcattatagtcagtcagcttctaggatcttttg ccaatcataattagggcagtaatcttaacctaaaggggtcaaggcaagct ctaaatggagtgacagttaagctctacctaagtgtattcactgctttatt gcaatgcaatttaggcatatatctgcctttcccctatcttgtctttttaa acagtttttgaaatacagactcctgtgagtctcccagtttagattttctc ggtgagtgtgtactagaatatttcatcttaggctctgaaaatcttatatt taaatgatgggatttgtagtcccattaagatagtaggtggtctctctatc acaataattttcacatgtcttttatctctctattatgcagccaaccccag cacaccctctgaaaccaaaaagccaaggagtgtggcccacttaacaggtt tgtgtctggaaggagtgggtgggggacaggaagcctttggaaaaatgtgg accaactgctcattttccattgcagctcttggaaagtatgactgacaact gtagttatcactcaagatcgttcttttgtctcttggatctcatgacccta atctgacaaaacccactgctatggaattgtgtatgatttgatcttgccca tgcaaagcatatactccttttaaccatttcaaagtccgcggggggggggg ggacttgtgatgtagatccaatgggagcagcagacggtgaaatgatgatc tatttcgagcaagaggagacagtcttggcatttgtgggctttcagcctaa gattataaagtgaaaaagtggtttagtacaattgctatgagcccctccac taggaaaataaacatccaaaatgtgggtaaatatcacaataagaggtcta gcatggctcttgtaatcacttggagactttagagaagtctgggtctataa acacttattgagtacttgctgtgtgttggtccatttaatcctgtatgaat acaaaggaaggggattaatccaaaagaagaggccaaggaatccatactaa atgaatggactacataatcatgagactttgtccagatgtagagctccaga gatccctttctttatggaggggtccctaagcccggaagtattggtactag tttaggtcataaagtgtttattacaaagtactgaagttatcctaataatc ttccccgagtttcacttgaggcacacaccgaatttgtcctaaacacatgg atgattaaaatgaggtggggaatttgacaaatgtgttgaagaaactcagc ggtggaccatgttttaactcctaagtcactcgcatcagctgtcattctgg ttagaacttttgttaagggaggccccacagttttgccttggaagcttaga gtgtcagatatgtgaacatttctggctgaatgctcctctctggtttatta cagggaacccccgctcaaggtccatccctctggaatgggaagacacatat ggaactgctttgatctctggagtgaagtataagaaaggcggccttgtgat caatgaggctgggttgtacttcgtatattccaaagtatacttccggggtc agtcttgcaacagccagcccctaagccacaaggtctatatgaggaacttt aagtatcctggggatctggtgctaatggaggagaagaagttgaattactg cactactggccagatatgggcccacagcagctacctaggggcagtattta atcttaccgttgctgaccatttatatgtcaacatatctcaactctctctg atcaattttgaggaatctaagaccttttttggcttatataagctttaaag gaaaaagcattttagaatgatctattattctttatcatggatgccaggaa tattgtcttcaatgagagtcttcttaagaccaattgagccacaaagacca caaggtccaacaggtcagctacccttcattttctagaggtccatggagtg gtccttaatgcctgcatcatgagccagatgggaagaagactgttcctgag gaacataaagttttgggctgctgtgtggcaatgcagaggcaaagagaagg aactgtctgatgttaaatggccaagagcattttagccattgaagaaaaaa aaaacctttaaactcaccttccagggtgggtctacttgctacctcacagg aggccgtcttttagacacatgattgtggtatgactatacaagggtgagaa aggatgctaggtttcatggataagctagagactgaaaaaagccagtgtcc cattggcatcatctttatttttaactgatgttttctgagcccacctttga tgctaacagagaaataagaggggtgtttgaggcacaagtcattctctaca tagcatgtgtacctccagtgcaatgatgtctgtgtgtgtttttatgtatg agagtagagcgattctaaagagtcacatgagtacaacgcgtacattacgg agtacatattagaaacgtatgtgttacatttgatgctagaatatctgaat gtttcttgcta >Human gaggtgtttcccttagctatggaaactctataagagagatccagcttgcc tcctcttgagcagtcagcaacagggtcccgtccttgacacctcagcctct acaggactgagaagaagtaaaaccgtttgctggggctggcctgactcacc agctgccatgcagcagcccttcaattacccatatccccagatctactggg tggacagcagtgccagctctccctgggcccctccaggcacagttcttccc tgtccaacctctgtgcccagaaggcctggtcaaaggaggccaccaccacc accgccaccgccaccactaccacctccgccgccgccgccaccactgcctc cactaccgctgccacccctgaagaagagagggaaccacagcacaggcctg tgtctccttgtgatgtttttcatggttctggttgccttggtaggattggg cctggggatgtttcagctcttccacctacagaaggagctggcagaactcc gagaggtaagcctgccggcagactgctgtgccctggaggcaccaggcata aggggatggagggcccactgcctggcttgcaaagtgcttttcaatccttt ttttttttttcttagaaatgggttgttctagttattctattctatagaca gagaaatggaggctcataggggtgaagggaatattttttactttgtaaag aatcagagcccaacagtttgtctttcctcagttgtcaactcagtctgttg aaccacttactgcctcatacctattgatttaaggagtaaaaaacaaacaa acaaaacttttaagttttgcttggaagtaaatttgttcagatgaccaaac taaatcttgaaaattggtacgattccaaatcacaaaactgagggacagtg aggcaaaattgcttggccaaagcccaagttagcagaacttctgaggtatt tggattctctttccagggcttggtttatttgacgattctgcctcttttgc ttaaagaattttatttttattatacatcttttctctttctgttttactag tctaccagccagatgcacacagcatcatctttggagaagcaaataggtga gtcttttttcgcatgtacattgagttcccaaagatgatcctcagcacaga actatgttaatggaatgccttaaattctgtcccacactttggtttctgta cactataagaggaattctggctaattcagaatctctggtctatgattcct tgagctgctttaaaaatgtgaagtgaattgaattgctcacaatcaatata gctgagcactgcacacaattcaaaatcacttccacttgactttggaaaga gacacacatatacaaccctggacctttgccccctgagaagtggctccagg cctgtccccttccacagacatcctggtcctggcacacacgccagtggctg taactcctgggaagagaaggcgaaatgaaggcagagacagatgtttctga gaaacgtcctttcctcttttaaatgccctaaagagattacattgaagctt tatttacaatatgttttataggatgtaagcttttaaaacatggataatca attccccacattagaatggtttttcttgaagtcactgagaagcttaaggg aagacttcagccatcattcaaaggatgttcagcttcacccacagaggcag tcatgaggtacccttgatcacaaaggaaaatcctttgtatgggagctagg gcaagaagatgataggttgaacactgatctcatgaaatttcagggcatag ttgtgcaagctctgtacagatagattttacagtgtgcttcactagtctaa gattcattgaaattttagatttagatttacaatgagattcattgaatttt tagaggtggaaggaagcttagaatatatctagtccagccatttcattttt tggaagagaaacaaaataccagatggattaggtgaatatgcccaagacca ctggtatgccattggggctagaaggaacacaggctcttgatgccttgtca cctacagggctttccagtactggactggtgcctcatgaaggagggaaact cctgtctctctgtagtgagcatggaataagaaatagatgaaaagcaggat acctctgcaaagactataggtagatctcatccttagaaactcagaataaa gaagaaggcagcactggccatctattaagtgaagcaagttccagtttctc cggatgtggctactgcacatgacccagtcgcctgtctactatcctagact gttaagttcttatatgagccactgttgggatgctctggcccctgcttctg tgtcctaatccttaaatgttagaaagtccatatgtacacaagcagagtgg aaatatttgatagaaaagtaaagcctctggaggacgtctgaaataagatc attttgaaaatctgtggaatgtgcctggtctgataggtatctaattattc atctttcacccactctctggagtcaatttagtgattaaaagtcaaatgat caggtgagtcagatccatctaccccagtaagcaattatgttctctagatc aacccaaataaaccagaaattggtaaatcatcacatggaaatcaaatcag taactttactaataagaaaagagatgcaaattaaaaatagggaataccaa ttttcatcaaattaataaagattaaaaaaaaacccagactgacaaagatt tgagagaaaagtgcctgtgcaaacattagtttcagccagtatacatgtaa attgctataactttttttgaagagcgctttagaaatacatagcaaaagct tgtaactagtagtttacgttgtaatcctatcgctaagaatttatcttaag aaaataatcagagatgtgcccgaagatttatatataaggatgttcattac ttccttatgagcctatttataataaataattcagaagaaacttgaatgcc tcatacgagattagttatatacattatggtgtattcagacaagcacataa taagcagcaactaaaagtgatgtatcagaaggacatttaatgctttgggg attgtgcactctgtattgttagataaaaagacaagttacaaaagggtatg tttagtttcatactgactatgtaaccatgtatgcacatttttatattctt aggaactggaaagttttacacactgacatactaacagtgattttctctgg gagatactattatagattatttatactttctaatttgctcctttttacat tttccatttttttccggttaactaattaaatcttttattcattcacatgt gtgaggctagatgttgggtgttggatgctgattgaggatgagccagccca gggggcctgtccttatggagcttttagcagggggaagaagctaaacaaaa gcaagcaaacagaccatgcaattccaaagttgtactaagtacaacctgag aatcttctttaaggaggtgttgtttacgctaacaccttgggggtcagaag cccgatgagtgaacggctgtgtgcatgtgctgtggagtggggtggggctg ggggcgggcatttcagcgctgggaacggcacatgcaaaaaccctggggca ggaagcactcataaccagtgtctaggatatccttgcaatttatttattcc agtataggtacagcagatattcttagtgagcatgttctgtaagaataacg ttgtactaggctctgtgagggatataaagacatgcaacatgtggttctct gttccgtttcctgccggtgcgtggctgagtgccagattagttgctcgggt ggtttgcggtgtaggaatgcagaggagactcagttttgcctttcctgttc gctaacacctcctacatggtgggttgtcttggatgactagagaggtcatg attgctcagctaatgttctctgtggattgggccattctcataccatatgt gagttttacaggctaatcacgaacctgacatagacaagcatctcctgtga atatttattgaagtctcagttagctgaattcattagctaagtggcagctg tctatcttgtctgaaacagttgaagtcagagaaatagtgtctgttttgag aactattgtctatccttccatttaatttgtccaacatttgctagtaacat gccctgtgttagaaactgggcttctctggtgaactcaacgtatcgtaagg aacttgtcgttatttgaggggaataagataatttatgtagttaccccaga gacaagaggagtcagaaatgacaagagaggggaagacagagggatcctag atttcaaaagaggggcagatcatatttgatcaaaaaaggcttgagagggt ttcccaaagaaagcggtaataggtagtccttatgggatgtttaggaattt gacacagaaatctgcttgaaagaacattacgggcagaagacatggcatga acaaaggcacagaggaaggaaagtgggggtatgtgtaggccacagaactc ccgttgtgtggcaatgctcagtgggagtaaggagataaggctggacagat gggctgggattgtgtgatggggatacttgtatacgacggcatcttcgggg aggagcaattaaaggtgtttgaggactcagtgatatgaggtgattgtcag aggtcttaagataattaatttggggcagattgtaaaatgtcactggggga agatctggtgacacgcagacagatcagtagtcttcttcagcctctgtcct gtaagtctttggaacaagagaatcagagtggacagagccctggaattggc gtcaggagagcagggtgtggtcccatctgtgatgctgactagctgggtct tcttggattagtcacccaacttctaagctcattttgcaatcgataaggtg ggacaataatacctacctcaaagggttgtggtgagttctaaatgcaataa ctgacatgacagctctttatgaataaatgtttatattaattatgttttat tgtaacattataatttatacatatgtcaatcttaatttggtttatttttt tacctctttgtttctgaaatatagactactatgaatcctgcagttcagac ctacatgattagtatatgttagactgttgccatttacggttttaaaatct tttttttaaaatgattggatttaaattcccaccaaaataatagttgctat ttcattttaacatatatttttcctctctctatgatacaggccaccccagt ccaccccctgaaaaaaaggagctgaggaaagtggcccatttaacaggtct gtatctggaaggtacaggtgagatttgggaaagcttttggcaaaaggcaa aatggctgcctggtttccattaccaggctctagagagttgttacttcctg gagacagtatttgaacccaataatactaacacttttcttgtcgagtcagt cttatgaacacactttgatcccctattctctgggcaaggccctcttctaa attggagacctggagctgattatgacatgacccctgcccataagaagctc attcttttcagtcattccaaagtcccttgggggattgggggcacagatcc aagaggtgagagatgagaaggtgaggaatgaggacttattttagtcagtg ctttcttggttttctggaagagcttcactctgctttgtccaagagtataa ataggagtgagaagggagactcaatcctggcacttgtagagtttcaatat gtgcctgtaaagtggagtgaaatgatatggtatgggcactcccattataa actacataaataatggaataaatatcacagaaagtggtaggctattgtcc ctggaattatttaggcagaaattatagaatgatccggtcacatcagcaga cacttactgaatacctgctgtgtgccaatttggggatgtgaagatgagta gcacatggctcttgcccccagggagcttgacatctaatagggaaagacag gcacttatgataatttcagtacttcagagatttaggttatgttcatgatg ttgtggggatacaaagaaaagggaactgtaccccaaattgaggggctgaa taattcttactgcaggtgagaagatggaccagatggtccctaagatcctt cccaactttagaactttagagttccttggatttggctttttccttcagga aaggacttcaaagcctagcagatttggtgctagttctgaagatagtaaaa tctttgttccagagagcaaatattttctcaataatttcttactgcaatgg attacgggtatatactattgttccaattgtgtggatgacaaaataggaca acgttgttgaggaaattctgtgatggatcaagttctgacccctcagccag ttctataccagctgtcattctgggtgaaacatttgttgaaggaagggccc acagttttgccttagaaacttagtttgttggatgcatgactattccttgt tgaaagctccttttggatttatttcaggcaagtccaactcaaggtccatg cctctggaatgggaagacacctatggaattgtcctgctttctggagtgaa gtataagaagggtggccttgtgatcaatgaaactgggctgtactttgtat attccaaagtatacttccggggtcaatcttgcaacaacctgcccctgagc cacaaggtctacatgaggaactctaagtatccccaggatctggtgatgat ggaggggaagatgatgagctactgcactactgggcagatgtgggcccgca gcagctacctgggggcagtgttcaatcttaccagtgctgatcatttatat gtcaacgtatctgagctctctctggtcaattttgaggaatctcagacgtt tttcggcttatataagctctaagagaagcactttgggattctttccatta tgattctttgttacaggcaccgagaatgttgtattcagtgagggtcttct tacatgcatttgaggtcaagtaagaagacatgaaccaagtggaccttgag accacagggttcaaaatgtctgtagctcctcaactcacctaatgtttatg agccagacaaatggaggaatatgacggaagaacatagaactctgggctgc catgtgaagagggagaagcatgaaaaagcagctaccaggtgttctacact catcttagtgcctgagagtatttaggcagattgaaaaggacaccttttaa ctcacctctcaaggtgggccttgctacctcaagggggactgtctttcaga tacatggttgtgacctgaggatttaagggatggaaaaggaagactagagg cttgcataataagctaaagaggctgaaagaggccaatgccccactggcag catcttcacttctaaatgcatatcctgagccatcggtgaaactaacagat aagcaagagagatgttttggggactcatttcattcctaacacagcatgtg tatttccagtgcaattgtaggggtgtgtgtgtgtgtgtgtgtgtgtgtgt gtgtatgactaaagagagaatgtagatattgtgaagtacatattaggaaa atatgggttgcatttggtcaagattttgaatgcttcctgacaatcaactc taatagtgcttaaaaatcattgattgtcagctactaatgatgttttccta taatataataaatatttatgtagatgtgcatttttgtgaaatgaaaacat gtaataaaaagtatatgttaggatacaa UMUC BIOT630 Lecture 9 Exercise – “Practice Version” – With Answers Question 1.a Are there any Evolutionary Conserved Regions (ECRs) between the genomic sequences for Mouse IGF2, Rat IGF2, and Human IGF2? To answer, use the sequences found at the end of the Exercise in conjunction with “multi-zPicture” (https://zpicture.dcode.org/multiz.php) with the Mouse sequence entered as your reference (i.e., “SEQUENCE 1”), the Rat sequence entered as “SEQUENCE 2”, and the Human sequence entered as “SEQUENCE 3”. Copy/paste the resulting “Dynamic Visualization” as your answer. Answer: Navigate to the main tool page via the link: Click on the “multi-zPicture” link. In the return page, click on the “SUBMIT” radio button: In the screen that opens after … Enter the Mouse sequence provided as the “REFERENCE SEQUENCE”: Then, enter in the Rat and Human sequences for the “COMAPRISON SEQUENCES”: After doing, click on the “SUBMIT” radio button. When the results page appears, click on the Dynamic visualization icon to get the expected answer: Question 1.b How many ECRs exist between Rat IGF2 and Mouse IGF2 (Hint: click on list of ECRs for “seq 2” in the Output files results section)? Answer: Go back to the results page obtained for Q1.a and click on the “seq2” hypertext to get the below expected answer: Question 1.c How many ECRs exist between Human IGF2 and Mouse IGF2 (Hint: click on list of ECRs for “seq 3” in the Output files results section)? Answer: Go back to the results page obtained for Q1.a and click on the “seq23 hypertext to get the below expected answer: Question 2. What are the Evolutionary Conserved Regions (ECRs) when “mVISTA” is used? To answer, save the sequences found at the end of the Exercise to individual “.txt” files. After doing so, upload the files into mVISTA and click on “Submit”. After, copy/paste the “PDF version” of the “VISTA Image” with the Mouse sequence represented as the reference. mVISTA url = http://genome.lbl.gov/vista/mvista/submit.shtml Answer: Question 3. What are the Evolutionary Conserved Regions (ECRs) between Mouse and Rat when the “UCSC Genome Search” tool is used? To answer, paste in the Mouse sequence found at the end of the Exercise into the sequence window of the tool, select the “Rat” genome in the “Genome” drop down menu, then click “Submit”. On the return page, click on the “detailed” hyperlinked text for the top hit. On the return page, scroll down and take a screen shot of the top portion of the “Genomic” results section and provide as your answer. url = https://genome.ucsc.edu/cgi-bin/hgBlat?command=start Answer: Question 4. What are the Evolutionary Conserved Regions (ECRs) between Mouse and Human when the “UCSC Genome Search” tool is used? To answer, paste in the Mouse sequence found at the end of the Exercise into the sequence window of the tool, select the “Human” genome in the “Genome” drop down menu, then click “Submit”. On the return page, click on the “detailed” hyperlinked text for the top hit. On the return page, scroll down and take a screen shot of the top portion of the “Genomic” results section and provide as your answer. url = https://genome.ucsc.edu/cgi-bin/hgBlat?command=start Answer: Question 5.a What does the conservation across species for Mouse, Rat, and Human “IGF2” look like using the “ECR browser” when Human is used as the “Base” genome and this isoform is selected: chr11:2150347-2162341 IGF2 insulin-like growth factor II isoform 2 To answer, copy/paste the browser view depicting Mouse and Rat against Human as the reference genome. ECR Browser = https://ecrbrowser.dcode.org Answer: Question 5.b What are the Evolutionary Conserved Regions (ECRs) based on your answer to Question 5.a? To answer, click on the linked option “ECR” located at the top of the browser page. Copy/paste screen shot of the ECRs as your answer. Answer: Sequences to use for Exercise: >Mouse GCTCACCTTCAGCAGCGTCCACTTCAGCAGCTCCCACTTCATCCGCTCTCCCGCCCTTCTCCTCCGATCC TCCTGCGCCAGCGGACCCGACCTTCGGCCTTGTGGTGAGCTCCCCGTGGCGCGCTCCCTCGTGCCCCAGC TCTTCCACTGCGGGGCACCGGCTACCTCGCAGTTTGTCCTCAGGGCTGCTGCGGAGTAGCGCGGGAGCGG GCTGCTGGCTTCTCCCTCGGCGGCAGCGCTCCTTTGGGCTGGGCGCTCCGAAACGCGCGCCACTTTGTGT GCACTCTGCAAACAACGCGGTGGTAGGGAAAGCGCCTGGGCGGCCAGGAAGCGGGAAGGGCAGAGCGCTC GGGCGGCTTGGAGCCTCCAACCCTACAGGTCGAGGGCTTGGGGAAGCGAGTGTAGAGCGCGGGCGCGGTG ATTGATGGTCGGGCCGGGAGGTTAGCCCCGGGTGCACTGGTTTCCGCCAGTAGTGGCCCTTTAGAGGCCT AGAGGTGCGGAGGTCGGGAGAGGGAAACAAGGTGCACCGGTTTAGAAGTGATTTTGACTTGGTAAAAATC ACAATGGCTTCTTATATCTGAAACCACCCGGGGAGAGTTTCTTAATAGCCCGGAATTATCGCATTGGCCC CTGGGGCCGAACCTGCAACTAAGCAAATTCAAAGTGCTGAGGACAGGGAACTGTTATCTGCTCATACTGG GTGGGGTCCCAAAGGCGCGCCGCTTAGAAGCACTTCAGTGAGCTCTGGTTTACAGGTCTGGTTTGAGCTA CGTTTCCCGCCCTGCTTCTTTTTCTGCTGAAGGGCGCAGACATTGTGCGGGGGCTCCACGCGGGGCCCAG ATTTGGCGTGGCCAAGCCAGCCTTCCCTTCATGGTGCACCTGGTGGCCCCCCAAAGGCTGCTAGGAGATC CCAGGCAAAGCAGAGGTTAGGGGCGCTTAGTAAGATCTATCTGGCTGCTATTCGAGGTGCCTCACAGCCC GCCCTGCCTGCTCAGGCAGGTGGAGGCTGCGCGCTCCCCCCCCCCCCCCCAGCCGATCCAGGGGACTGTC CCCTGCTTAGCAAGTAGGTCCCTGTGAGAACCCTCTACCCAGCTACCTGCGCCTTAACTCAGTCGGCCGG GATGCGCCTACCTTGTAACCCTCTAACTGAGCACAGGTCCTGCCACTACCACTGAGGCCTAGGGTGCCAC GCACGTTGGTTCGCACCACGCGCCCATGTTGCACAAGCACAGGGCACCCCGGAAACATGACATGGGACCT CGATTTTTAGGGATAGACACCCAGGATTTAGTGGGCCTGTTGGGGCGCACAGCCCCCCCCCATCTTTGCC ACCAGGCACTCCACTCCCCCCCCCCACCGGCAACCCCCGTGCCCAGGGCCCCCGACCCGCATTCCCCGCG TGGCTCGAGTTGCGGGGGCGGTCCCGGGGCGGGGCGAGGGCCCTGCGGACGCCCATTGGCGCGGGCGTAA GGCCAGCGGGGCCCGAGCGGGCGCCGAGCCGCGGGGTGGCGCGGCTATAAGAACCGGGCGTTGGCGCCCG GAGTTCGCCTGCTCTCCGGCGGAGCTGCGTGAGGCCAGGCCGGCCCCCGGCCCCCCCTTCCGGCCGCCCC CGCCTCCTGGCCCACGCCCGCCCGCGCTCGGCCCGCCAGCGCCTCCATCCGGGCTGGCGGCCCCGCGTCG ACGCCGTCCGCCACCTCGCTGCTGCTAACTCCTGTGCAGGGCGCCGTCGGCGGGGCCGCGCTCCGTCGGG CCTGCGGATCTCCCCACCGCCTCCTCCTCTATCTACCTCAACACCCCATTCCTGCTTCGCCAGAGGAGGC GGTCCCCACCGCAGGCAGTCCGGCTTGCAGGTCGCCGGCGTTGTCATCCCCCGCGCTTCCCCTCCCAGCC CTCCCCGGTGCGCAGCCCGGCTGCTCCCCTCTTTTCGCTGCAGTCCCGAGCAGCTGAGGCGCCGCCACGC CTGTCCCCCCCACAAGAAGCCCGGGCTTACGACGGCTGAGGGCTCCGTCGACCCTAACCGAGCTGGGTGC CCGTGGCCGGGGTGACGCCTCCATTCCTCCCCCCTCAACACCGTCCTCGGTCCTTCGAAGTTGCATCCTC TCCTCTGCTTAGGGTGCGCCCCCCCTCGCGCACCCGCTTACCCGCCACCTTTCCTAAGCTCCCCTCCTGC CCCCTCCCCGTTCCTCCTCGCCTCAGACTCCCTCCCCCTCACGTCCGCCCTCTGCCTTCGCCTACCCAAG TGGATTAATTATACGCTTTCTGTTTCTCTCCGTGCTGTCCTCTCCCGCTGTGAGCCTACCCGCCTCTCGC TGTCCTCTCTCCCTCTCTCCCTCTCTGTGGTCCCCCCCTTTCACGTTCACTCTGTCTCTCTCACTATCTC TGCCCCCCAACTATCCTTGATACAACAGCTGACCTCATTTCCCGATACCTTTTCCCCCCCGAAAGTACAA TATCTGGCCCGCCCCAGCCCTAAGATACCCTAAAGAAGCAGAAGAGACGCCCCCCTCCCCACCAAAAAAG CCATCTCCCCGTTCTGTCCCGTCGCACATTCGGCCTCTGCGACTCGGGCAGAGCGGCGCTGGCAGAGGAG TGCCCGGCAGAAGGGCCTTCGCCCGCTGTTCGGTTTGCATACCCGCAGCAGGGAGATGGGCGGCAGCGTC GCCGGCTTCCAGGTAAGGGCTCTCAGGGGCCCTGGGAGTGGGGGGTGCGCAGAGCCCGGCTTGGGCATGG CGGGACTGGTGCGGTGGGTGGGGGGCTGGGGGCTCGTTCCCTAAGCAAACTTTTGTTCCCCCTTGCGCCA TGGAGTGGGGGAAGGGGCGGGGGTATGGGGGCTGTTGGGGAGGGGGTGTGTAAGTTGAACTCACAGGAGA CTTCCTGGTGCTTTACTGTGTGTTGGGGAAGGGGGGGATGGATGAGCTGCATAAAATGTACGATTCGTGC TGTGCAGGCCCTGTACACCCCCCCCCAAGGGTGCTGAGTGATGAGGAGGGGGGCAGAGTCTCCGAAGCCC ACCCTGGTATGTTGACTTTGTGCCAATGAGACAGGAAGGAAAAGTGGGTGGGCGGGGCCAAGAGCCCCCC AAAATTGGTAAAAGAGTCAAGCCAGACCCCACAGTTAGCTTTTCCCCCCACCCACACACCCCAACTCTGT CCCCAACATTCCCAACCTGGAATGTTGGGGGGAGAGGGGACGGTTTCGGGCTCAGAAGGGCAGAAATGTT ACAGTTCAAAGAGAAATAACTGCCCCTGCCTTTTACTGGGTGTAACTTTCCATAGGAGGATGTGGGAAGG AGCCCCCTCCCTCCCCCCATTGGTCTGTGCAGAAAGGGCTGGGGGCGCACGATGAGGCCCCCCTCCCACT GGTGGTGCTTTGCTAAGGAATGGTCCAAGGCTAGCTCTTGGGGGTGCAGGAGAAAAGGGACTGGCTGGAA GGAGGGAGGGGGCGGGTGCAAAGGGGGCGGGGGGAGTGGTCAGCAGGGAGGGGGTGGGGGGTAGGGTGGA GCCGGGACTGGGAGGAGCCACTCAGACATAAAAAGCGGAGGCACTGACCAGTTCGCAAACTGGACATTAG CTTCTCCTGTGAGAACCTTCCAGCCTTTTCCTGTCTTCATCCTCTTCCAGCCCCAGCGGCCTCCTTACCC AACTTCAGGTAACCAGGGCTGTGGAGCCAGGACCCTGCTGCCATCCCCACTCCGGCTTGCCCAGTGGGGT GGTCAGGGCACCGGGTAGCCTGAGGCCTCTGCCCTTGCAGCCGGGCCTGCTACCTCTCCCAAGCCTTCGT TCTCCCTCAGCCTCTGTCCTATGCCCCCCACTAGATCCAGGCTTCCTTACCTGCCCAGTCCTCTACTTTT CCTTCCTCTCTGCCTCCCCACTCCAACAAATTCGGGCCTTCCCTGGGCCCTCACCTGTCCCTTGCCTCCT GGTCAATCTCCTGCTTGCTTCTCCTGGACACCTGGGCTGAACCAGCCAGTGTGGTCTTGGCTTTGGGAAG GGACTTCGAGGTCAGCTCCGTCTGCCTGCCCCCCGCCCCCAGAACTAACTGCTGTAGCTATAGCCTGCTG ATCGCAAGTGGTTGGTGGCACAGAGGGTATTGGGAGGATCGGTCTGGGTCTTGATGAAGTGGGCAAAGAT GACTTTTAGTTACCCAAGGGAGCTCGAACAAATTTGTTTCTTGATTATTCCGCACTGGTCTCTCACATGT TTCTTTGCAGCAAGAAATCCCCTGGGCCTATGGAGCAGAGATTGAGGCTGCGGCCTGGAGGGCTCTACTT AACTGAAGGGTCAAGGGTGGCTTACTGTGGGTCATCGTGGCCTCCCACCACTAAAAAAGCTAGCTAACCA TTGTCCCCGCCTGTAGCCACATAGCCTGTGGAGGGGGTGTGTCCCTCCAAAATGGGGTGTTTAGCCTCCC AAGGACCCAAACTGCTGCCCTGGGTAAATTTAGCTCTTATCAGAAAGATGATTCTATGGGCCATAAAGGG ACCCTCTAGGGTCCCTGACCATGGATGGCAAGCTCTGAGGTGCCCAGGAAAGTCTATACTTGGAGGACTG GGTGACCCTGGAGAGCAAGGCTCACAGGAGTGCTGGCTATCCCATTGCGCTAACCATTGGATTCTGACAT GGGCCCTGCTCACCATGGCCTGGGAGGTGGGGTGGATGGGTGGGGCTGGCTCTGCTGCTTAGGGAGGGGT GGCCAGCTGGGGGGCGGGTAGGTTGGGAATGGTCCCTTTGGATGCACATGCTCCATGGTGGGTGGGGTGG AGCTATCTGCCATCTTGGCCAATGCTTGGAGGCCAGAGTAGGAGTGGCAGTAGTGATGTGCCCTTGGGCT GGCCCCACCCCCATGATGTCATAGCATGGAAACCAGGTCCAGTGTCCAGCACTGGCCCCTGCTGCTGTGG GTGTGGTTAAGCTGCAATTGCGCCCATGGATGGGTGGTCCTCAAGGGTTTCTTACAGTTCAAAGCCACCA CGGGGTGCCTTCCTGGGGTCCATTGAATTTCTCCACACTCAAAGTAGTTTTTGACCTCAGCAATTCAAGT CCCCCTGTTTTCACAGGTTAAATAAATAGTGACATGGCTGTCTTTCAGATTCCAATTGCATTTTTAATTA AGAGATGATAGGTGTCTTTGGTGGGGCCTGGGTGGGGGTGAGCCATTCTCCTGGGATTAGGGGTGTGGCT TGGGGGCCTCTAGCTCAAAGCCTGCGTTTCTTTCTCCAGGTACCAATGGGGATCCCAGTGGGGAAGTCGA TGTTGGTGCTTCTCATCTCTTTGGCCTTCGCCTTGTGCTGCATCGCTGCTTACGGCCCCGGAGAGACTCT GTGCGGAGGGGAGCTTGTTGACACGCTTCAGTTTGTCTGTTCGGACCGCGGCTTCTACTTCAGTATGTAA CTCCAGAGAGGTGGGGGCGGGGGAAGCAAGCCTCCCTGGATGGGGTGACAATTAGTACACTGGTCTGGCC TTGCTCTTAAGCTACCCCTTCCCCCCCCCCCCCGGGCAAGGTCGTAATCTCAAAGTCACAAGTCTGATAG TTTTAAAAGTTTTTAAAGTTGATAAAAGCAAAACCAAATGTGGATGTCAAGTCTCAGCCAGGTTGAGAGT GCACGTGTAGTTTGGGGGTACAAGGGGAGAGCTGGACCTTGGCCATCCAACTTGAGGGGTTCCCAGGACT ACAACATGGGGTGCTTGCCTGCTTTCAATGTTCATGCTTCTCTTGATTTCCCAGTTGGGGAAGGGGCTGG GCATGGGCTCACTGCTCACAGAAGGCAGTGATGGGGACACATGGGTGGGGCATGCCTGGGAGGGGCAGGG GCACCAGAATGGATGACTATCCTTGCTGGCATGGGCCACTCTATCTTCCTCGCCTGGTCCTGGACACGCA GCCTCTTCTTCTTCACTTCTGCCTACCTGCCTGTGAACTGCTCTGGAAACAACTACTGCTCATGGGTACT GCAGGACCAGTCCTTGGTCGAGAGCCACTCCTGCACGGAGGGGCTTCACAGATAGGAGGGCCCCAAGCCC AACCTCGGACCGTGGGGGAGAGGGGGAAGACGGGAGAAGAGAAGGGAGTGGTTTTTGGGTGCCTCACTCC TCCCCTCCCGTCTTGTTCTCTCCTGCCCTATCTTCCCTTCCTGTCACAGTTCAGCGATGGGGGTTGGGGG TGGGCCCCTCAGGACGGGCCCCAAGTACAGGGACCACACCTGTGTCCACCATGGCCCCCTCGAGGGGCTC ACCCCCTCCTCCTCCCTGTTTTCCAAACCACTCCAGTGTGGCCTCTGGTCTCCATGGAGTCTGTGGGACA GTGAAGCTGTGCTGTGTTAGTTACCAGACAGGAGGCACCAGATGCCAGGGGCTGGTCCTGCAGCATGCAC CTTTATTTTTCCTTTCTGGGCATCTTGGCCTGTCTGGCTCACACTTCTACCACATCCCTGCAGGGTGACC TAGTATCCGGCCAGGGTCTAGTGGAAGGGCAGGGGTAGTTCCTTCTTCAGCCTTCCCCTCCTGGGGGGCG GGGCTGGGGTGGCCACCCCAATGGGTGCTGACCCCAGTAGACACTAACTGAAGTTGTCTGTCCTGTGGAA CTTCCAGGCAGGCCTTCAAGCCGTGCCAACCGTCGCAGCCGTGGCATCGTGGAAGAGTGCTGCTTCCGCA GCTGCGACCTGGCCCTCCTGGAGACATACTGTGCCACCCCCGCCAAGTCCGAGAGGGACGTGTCTACCTC TCAGGCCGTACTTCCGGTAGGTAGCGAGGTGGGGTTGAAGGAATTATGGGGTGAGACAAAGAGACCACTC ACTTCTTGATTTGGGGTACCACGAAGCCCCCCTCACACATTTTACTGTCAGCCTCTGCTCATTAACATAG ATATTTCAGGGAAGTTGCCCTGAGGTGGGGTGACTCTAGTGGGGCTGGGGATATGCGACACCTAGATGGG AGCTCAGGCTAACATGATACCCTGAAACCTGCTGACTAGCACCTCCTCTCCAGGACGACTTCCCCAGATA CCCCGTGGGCAAGTTCTTCCAATATGACACCTGGAGACAGTCCGCGGGACGCCTGCGCAGAGGCCTGCCT GCCCTCCTGCGTGCCCGCCGGGGTCGCATGCTTGCCAAAGAGCTCAAAGAGTTCAGAGAGGCCAAACGTC ATCGTCCCCTGATCGTGTTACCACCCAAAGACCCCGCCCACGGGGGAGCCTCTTCGGAGATGTCCAGCAA CCATCAGTGAATCAAATTATGTGGTAATTCTGCAATGTAGTACCATCAATCTGTGACCTCCTCTTGAGCA GGGACAGTTCCATCACGTCCCACACTAAGATCTCTCTGCTCCACTCCCTCCCCAGGTTTCTCCTTACCCA CCCCCATGCCCCGCCTCCCCACATCAGGCTGTTCCCCTTGCCCCACACCATCGGGCAAGGGGATCTCAGC AGTTCTAAAAAACCAAATTTGATTGGCTCTAAACAACCCAATTGACACCCCCCAAATTATATATTCACAT TAAAAAAAACTTTAAAAACTACAGTCCCTTTAAAAACAAATTGGCTTTTAAGAAATTCCATAACTGATAA TCTAAAAATTAAATAACTAAAGAAATTAATTGGCTAAAAACATACTAAAAATTAATTGGCTTAAAAACAA TTGGCAAAATCAAATAATTCGGCGCCCCCCCCCCTTCATCTTCTTTCCATTTGGATCTTTAGTCAAATTG GCTAAGACTTGGATCCCAGAACCCAAGAAGAAAGGAAGGGGACCCAAAATTTTGCAGGTGGCATGTCATT GCTTCAGTGCCCTCTCCTTATCCCAAGCACCTTTAGTGTAAAGCTGGCTGAGAACAACAATAGCCGCCCA AACTCTTTCTTCACTGGTCATTCCATCACAAATGTCCCCCATGTCACCAAGGGGGCTGGGTGAAAGAACC CAAGGAGAAGAACAGAACATGAAAACTGGAAATAGAACTTAATTGGCACAAGCCCCCAGTCCCAAAATCT CACTTTTCCCTACCTACCCTAAAAAGCACATGATTATACTCACACCCACAGGCATTAACACACACATACA CATAACACATATGCACACACACACACACACACACACACACACACACACAGAGGTATGCACACCCACATAA TTGGATGAAAACATTGAAATGGCTGAACAACTTCGATTTGAACCACATTCCCCAAAACAAGGCCCATCCC AAATTTCCTGAGCAGTTTGCATGGTTTGAGCTCTCCTCCCAATCCATCTAGTTTCTGCTGCTGCTTATTG GCTCTATGTGTGACCAGGCTGCTAGTTCTGTCTTTCTCAGTGAGGGAGGTGTTCTAGCAAGGCTCAATTC CATCTAAGCACCTGTCCACATGGTGAGCCCGGTGCTCCCCAACCACCCCCGCCATGAGGACCAATGACTA GCCAGTGTAGAATAAGTTTGGCCAGATAAGGAGATGGCACTGCCAAGTGATACATGCTGCCCAAGTAACC TGACCCCTAGTTGTGCTCCTGGGAGGAAAGATCTGGGGGACAACCCCTACCCTGAGCACACCTATGGGCC ATCTTTGTCAGTCTCCTGGGGAGCCCCCGCTTTTTAGGGGCTCCCCAGGAGACTCACACTGATGTGGGGA GTGTGGGAAGTCTGGCGGTTGGAGGGGTGGGTGGGGGGCAGTGGGGGCTGGGTGGGGGGAGCTATGGGTA GGAAGTGGTCCCAGAGAGGTTTTAGGTGGAAGAATCAGGAGGAGTCACAGGTCAACTTGCAGAATTACTG AAGAATTAGGACCCCAAATTTTATGCCAATTGATCTATTCCCCTCTTTTTATTTCTGGGGCCGGTTTTTT CCTTTTTTTTTTAATCCCTCCTTAGCTTTTTATGCGCTCATAATCAATTGTACCCATTCCCTACATAACG GGAGCAGTGATCAGGTAATGAATGCATCGAGCCATCAACACCAGCTAGAGCCATCAACACCGGCTACCAC AATGTCCTGCTCTCCACAACCTTGATTTTTTTTTTTATCTCTCTCTATCGCTTGGCCTGAGTTGGGAGTG GAGTCTCTGTGGGGTGCTGGCCACGCACCCACAGAGAAATAAAAGGAATTGAGAAGGTCGCTACCTGGCC TGACTTCTGGGGACAGTGGCTGGTCCCCAGAAGTTCTGAGGAGTGGAGGGGGCGTGGGGCAGTGTCCCCT CAGGTGTTAGGAAGGTGCTCGGAGGCCACAAAAGATGGGGCTCCCAGCTGGCCACTGCCAGTTGGGGGGG AAGGGGATAGAGATGTGAGAGTAGACAGGTTACATCAGGCGGGAGCAAGTGGCTGCCTTCTGAGCATTTG GGGGAGAACCTCTCCCGTCCCTCAGTGTCATCTTGCCCACTCCTCAGCACCCCACCTTGCCCCCAGGAGG TCTGGAGCTCTACAGACCTCCTGGGGGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTA AAGCCTACAGAGCCAGGAGAACTGTATACACGGGGTCATCTGGGCCCTGGGGCCTGAGGGTCTGGCGAGC CGTAGCAGCCACTCCACGGTGCCTAGGACTGCGGCGGGGAACAGGGCGGCTGGAGGTTTACCTCACCCCC ACTTCTGCTTCCAGTGCAGTCCCCCTGCCCAACAGTCCAACTAGCAATCTAGAGGCCTGAGGCTTCTGGG CCCAGGTGACAGGACTGGCACCACCCTGGGGGCGGTGTGTGTCAGCCAAGCATGGCACAGAGGGTCCCTC AGCAAGTGCCTAAAGAATGGGCCATTTGGAACATTGGACAGAAACTCAAAGAGTAAATTGTTATAATTGG AGACTATGAATTGGCCTGGTATCCAAAACATCTCGAGGCACCCTAAATTACCTGCCCATTTGGCTGGACA CCCACCCAGTGTTAATATGCCTCGTGGGATGGGTGCTTTCAGGGGCATTTGCTGACCACCCTCTGTGTCC CCACATTTGCAGTTCTCCCCATCATAGGTCACCTTGATGCAGGCACCTCCCTGGCCTCCTATGCCTAGTG TGGCCCTCCATCTTGTCTCTTCCCTACTGTCTTCAGTGGGATCCCCTCTTGGGTCCCTCCCTTTGTCATC ATGTGAAGACTTCCCACGCGTCGAACGCCATATGTCACCTGTGCCACTGTCCATGTCATCCAGCAGTGGC CCCAGGTGTTTGCCTCAACTCAGTCCCTCTAACATGCATTTTCTGGCAAAATCCAAAGCTTGGGTTTTGT TTTTAACCTGTTAACGCTCGCAAACCTAATAAAGCATTTAAAATACTA >Rat GCCTGCTCTCCGGCGGAGCTGCGTGAGGCCAGGCCGGCCCCCGGCCCCCCCTTCCGGCCGCCCCCGCCTC CTGGCCCACGCCCGCCCGCGCTCGGCCCGCCAGCGCCTCCATCCGGGCTGGCGGCCCCGCGTCGACGCCG TCCGCCACCTCGCTGCTAACTCCCGTGCAGGGCGCCGTCGGCGGGGCCTCGCTCCGTCGGGCCTGCGGAT CTCCCCACCGCCTCCTCCTCTATCTACCTCAACACCCCATTCCTGCTTCGCCAGAGGAGGCGGTCCCCAC CGCAGGCAGTCCGGCTTGCAGGTCGCCGGCGTTGTCATCCCCCGCGCTCCCCCTCCCAGCCCTCCCCGGC GCGCAGCCCGGCAGCTCCTCTCTTTTCGCTGCAGTCCCGAGCAGCCGCGGCGCCGCCACGCCTGACCCCC CCACAAGAAGCCGGGGCTTACGACGGCTGAGGGCTCCGTCGGCCCTAACCGAGCTGGGTGCCCGTGGCCG GGGTGACGCCTCCATTCCTCCCCCCTCAACACCGTCCTCGATCCTTCGAAGTTGCATCCTTTCCTCTGCT TAGAGTGCGCCCCCCCTCGCGCACTCGCTTACCCGCCACCTTTCCTAGGCTCCCCTCCTGCCCCCTCCCC GTTCCTCCTCGCCTCAGACTCCCTCCCCCTCACGTCCGCCCTCTGCCTTCGCCTACCCAAGTGGATTAAT TATACGCTTTCTGTTTCTCTCCGTGCTGTCCTCTCCCGCTGTGAGCCTACCCGCCTCTCGCTGTCCTCTC TCCCTCTCTCCCTCTCTGTGGTCCCCCCCCTTTCACGTTCACTCTGTCTCTCTCACTATCTCTGCCCCCC AACTATCCTTGATACAACAGCTGACCTCATTTCCCGATACCTTTCCCCCCCCCGAAAATACAGTATCTGG CCCGCCCCAGCCCTAAGATACCCTAAAGAAGCAGAAGAGACGCCCCCCTCCCCATCAAAAAAAGCCATCT CCCCGTTCTGTCCCGTCGCACATTCGGCCTCTGCGACTTGGACAGAGCGGCGCTGGCAGAGGAGTGCCCG GCAGAAGGGCCTTCGCCCGCTGTTCGGTTTGCATACCCGCAGCAGGGAGATGGGCGGCAGCGTCGCCGGC TTCCAGGTAAGGGCTCTCAGGGGCCCTGGGGTAGGGGGTGCGCAGGGCCCGGCCTGGGCACCGCGGGACT GGTGTGGTGGGTGGGGGGCTGGGGGCTCGTTCCCTAACCAAACTCTTGTTCCCCCTTGCGCCATGGAATG GGGGAAGGGGAGGGGGTATGGGTCCGTTGGGGAGGGGTGTGTAAGTTGAACTCACAGGAGACTTTCTGGT GCTTTGCTGTGTATTGGGGAAGGGGGAAATGAACTGCTTAGAATGTATGATTTGTATTGTGCAGACCCCG TACCCCCTCCCGCAGGGTGCTGGGTGATGAGGAGGGGGGCAGAGTCTCTGAAGCCCACCCTGGTATGTTG ACTTTGTGCCAATAGGACAGGAAAGAAAACTGGGTGGGCGGGGCCAGAAGCACCCCAAAATTGGCAGAAG TCAGGCGAGACCCCACAGTTAACTGTTTCTCCCCACCCACACACCCCCCAACTCTGTCCCCAACATTCCC AGCCAGGAATGTTGGGGGGGGGCGGTTTCGGGCTCAAAAGGGCAGAAATGTTACAGTTTTGAGAGTAACT GCCCCTGCCTTTTACTGGGTGGAACTTTCCATAGGATGATGTGGGAAGGACCCCCCTCCCGCCCCCATTG GTCTGTGCAGAAAGGGCTGGGGGTGCACGATGAGGCCCCCCTCCCACTGGTGGTGCTTTGCTAAGGAATG GTCCAAGGCTAGCTCTTGGGGGTGCAGGAGAAAAGGGACTGGCTGGAAGGAGGGAGGGGGCGGGTGCAAA GGGGGCGAGGGGAGTGGTCAGCAAGGAGGGGGGGTGGGGGTAGGGTGGAGCCGGGACTGGGAGGAGCCGA CTCAGACATAAAAAGCGGAGGCACTGACCAGTTCGCAAACTGGACATTTGCTTCTCCTGTGAGAACCTTC CAGCCTTTTCCTGTCTTCATCCTCTTCCAGCCCCAGCGGCCTCCTTATCCAACTTCAGGTAACCAGGGCC ATGGAGCCAGGACCCTGCTGCCATCCCCCCTCCGGCCTGCCCATTGGGGTGGTCAGGGCACCGGGTAGCC TGGGGCCTCTGCCATTGCAGCCGGGCCTGCTACCTCTTCCAAGCCTTCGCCCTCCCTCGGCCTCTGTCCT GTGTTCCCACTAGCCCCAGGCTTCCTCACCTGTCCCGCCTTCTACTTTTCCTTCCCCTTTGCCTCCCCAC TCCCCACAAATTCAGCCCTTCCCTGGGCCTTCACCTATCCCCTACCTCCTGGTCAGTCCCCTGCTTGCTT CTCCTGGACACCTGGGCTGGACCAGCCAGTGTGATCTGGCTTTGGGAAGGGACTTCGAGGTCAGCTCCGC CTGCCTGCCTCCACGGCACCCCCTCACCCCTAAGGATTAACTGCTGTAGCTATATAGCCTGCTGATCACA AGTGGTTGGTGGCCCAGAGTGCATTGGGTGGGCCGTTCTGGGTCTTGTTGAGGAGGTGGGCAAAGATAAC TTTAGTTATCCGAGGGAGCGCGAGCAAATTTGTTTCTTGATTATTCCGCACTGGTCTCTCACTTGTTTCT TTGCAGCAAGAAATCCCCTGGGCCTGTGGAGCAGAGGCTGGGGCTGGGCCTGGGGGGCTCTACTTACTGC AGGGTCAAGAGTGACTTACTGTGGGGCATCGTGACCTCCCACCACTAAAAAAAAGCTGGCTAACCATTGT TCTGTAGAGCCACACAGCCTGTGGAGGGGGTGTGTCCCTCCAAAATGGGGTGTCTAGCCTCCCAGGAACT CAAACTGCTTCCTCTAGTACCCTGGGTAAATTTAGCTCTTATCAGAAAGACGGTCCTATGGGCCATAGAG GTACTCTCTAGGGTCCCTGACCATGGATGGTAAGAGTTGAGGTCTGGATAGGACTTTAGGTTCCTTTTGA AGCTCTGAGGTGCCCAGGAAAATCTATACTTGGAGGACTGGGTGACCCTGGAGAGCAAGGCTCACAGGAG CGCTGGCAGGCCCATTGCGCTAACCATTGGATTCTTGACCTGGGCCCTGCTCATCACGACCTGGGAGGCG GGGTGGACGGGTGGGGCTGGCTCTGTTGCTTAGGGAAGGGTGGCCAGCTGGGGGGCGGGGAGGTTGGCGA CTGGTCCCTTTGGATGCACATGCTCCACGGTGGGTGGGGTGGAGCTGTCTGCCATCTTGGCCAGTACTTG GGAGGCCAGAGTAGGAGTGGCAGTGGTGATGTGCCCTTGGACTGGCCCCACCCCCATGATGTCATAGCAC GGAAACCAGGTCCAGTGTCCAGCACTGGCCCCTGCTGCTGTGGGTGTGGTTAAACTGCAATTGCGCCCAT GGATGGGTGGTCCTCAAGGGTTTCATACAGTTCAAAGCCACCACAGGGTGCCTTTGTGGGCTCCATTGAG TTTCTGCACGCCCAGAGCCAGGTTTTTTTTTTTTTTTTTTACCTCAGCAATTCAAACCCTCCTGTTTTTG CAGGTTAAATAAATAATGAGACGGACGCCGTCTTTCAAATTCCAATTACATTTTTAATTAAGAGATGATA GGTGTCTTTGGTGGCGGGGAGAAGCCAGACTCTTGGGGATAGGGGTGTGGCTTGGGGCCTGTAGCTCAAA ACCTGCGTTTCTTTCACCAGGTACCAATGGGGATCCCAGTGGGGAAGTCGATGTTGGTGCTTCTCATCTC TTTGGCCTTCGCCTTGTGCTGCATCGCTGCTTACCGCCCCAGCGAGACTCTGTGCGGAGGGGAGCTTGTT GACACGCTTCAGTTTGTCTGTTCGGACCGCGGCTTCTACTTCAGTAAGTAACTCCAGAGAGACGGGGGAG GCGGGAGCAAGTGGGGTGGGGGGGAGGTGGTAACAGCACAGTGGTCTGGCTAAGCTACCACCCCACCCCC CCTTGGGCAAGGCGGTAATCTCACACTCACAAGTCTGATAGCTTTAAAAGTTTTTTTCAAAGTTAATAAA AGCAAAACCTAATGTGGTTCCCAGGTCCTAGCCAGGTTGAGAGTGTACACATAGTCTGGGGCTCCAAGGA GGGAGAGCTGGACCTTGGCCTACACCATGGGGTGCTTACCTGCTTTTCAATGTTCATGCTTCTCTTGATT TCCCAGTTGGGGGAGGGGCTGGACCTGGGCTCACTGCTCACAGAAGGCAGTGATGGGGGTGGGGTGGGGG ACACACGGGTGGGGCATGCCTGGGAGGGGCAGGGGCACCAGAATGGATGACTGTCCTTGCTGGCCTGAGC CACTCTATCTTCCTCACCTGGTCCTGGACATGCAGCCTCCTCCTCTTCACTTCTGCCTACCTGCCTGTGA ACTGCTCTGAGTGCTCAAACCTCTGGAAACTACTTCTGCTCCTGGGTACTGCAGGACCAGTCCTTGTTCA GGGAGCCAATCCTGCACGGAGGGGCTTCACAGATAGGAGGGCCCCAAGCCCAGCCTCGGACCGTGGGGGA GAGGGGGAAGACGGGAGAAGAGAAGGGAGTGGTTTTTGGGTGCCTCACTCCTCCCCTCCCGTCTTGTTCT CTCCTGCCCTATCTTCCCTTCCTGTCACAGTTCAGCGATGGGGGTTGAGGGTGGGCCCCTCAGGCTCAAG GTGACACCAGGCTGGGGGGCCCCAAGTCCAGGGACCACACCTGTGTCCACCATGTCCCCTCGAGGGGCTC ACCCCGCTCCCTGTTTTTCAAACCACTTCAGTGTGGTCTCTGGTCTTCTTGGAATCTGGGACAGAGAGAC TGTGCTGTGTTAGCTGCCAGGCAGGAGGCACCAGATGCCAGGGGCTGGTCCTGTAGCACGCACCTTCGTT TTTCCTTTCTGGGCATCTTGGCCTGTCTGGCTCCCACTGCCACCACATCCTTGCAGGGTAACCTAGGGTC CAGCCAGGGCCTAGTAGAAGTTCAGGGGAAGTTCCTTTCTTCTTCAGCCTTCCCAAGGGGGAGGGTTTGG GGAGGCCACCCCAGTGGGTGCTGACCCCAGTGAACACTAACTGAAGCTGTCTGTCCTGTGGAACTTTCAG GCAGGCCTTCAAGCCGTGCCAACCGTCGCAGCCGTGGCATCGTGGAAGAGTGCTGCTTCCGCAGCTGCGA CTTGGCCCTCCTGGAGACATACTGTGCCACCCCCGCCAAGTCCGAGAGGGACGTGTCTACCTCTCAGGCC GTACTTCCGGTAGGTAACAGGGTGGGGTCGAAGGAATTGTGGGGTGAGACAAAGAAATCACTTGCTCCTT GATTTGGGGTGCCATGAAGTCCACCCACGTTTTACTGTTGGCCTCTGCCCATTAACATACATGTTTCTAT AGGACTCTAGTGGGGGTGGGGTATATTGACACCTGGATGGGAGCTCAGGCTAACTCGATACCCTGAAACC TGCTGACTAGCACCTCCTCTCCAGGACGACTTCCCCAGATACCCCGTGGGCAAGTTCTTCAAATTCGACA CCTGGAGACAGTCCGCGGGACGCCTGCGCAGAGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCGCAT GCTTGCCAAAGAGCTCGAAGCGTTCAGAGAGGCCAAGCGCCACCGTCCCCTGATCGTGTTACCACCCAAA GACCCCGCCCACGGGGGAGCCTCTTCGGAGATGTCCAGCAACCATCAGTGAACCAAATTATGTGGTAATT CTGCAATGTAGTACCATCAGTCTGTGACCTCCTCTTGAGCAGGGACAGCTCCATCATGTCCCACACTAAG GTCTCTCTGCTCCACTTCCCTTCCCAGGTTTCTCCCCACCCACCCCCATGCCCCGCCTCCCCACATCAGG CTGCTCCCCTTGCCCCACACCATCGGGCAAGGGGATCCCAGCAACTCTTCAAAACCAAATTTGATTGGCT CTAAACAACCCAATTGGCACCCTCCAAATTATATATGAACATTAAAAAAAAACTTTAAAGCATATAGTCC CTTTACAACAAATTGGCTTAAGAAACTCCATAACTGATAATCTAAAAATTAAATAACCAAAGAAATTAAT TGGCTAAAAACATACTAAAAATTAATTGGCTTAAAAACAATTGGCAAAAATCAAATAATTTGGCCCGCCC CCCCCCCTTCATCTTCTTTCCATTTAGATCTTTAGTCAAATTGGCTCAGACTTGGATCTCAGAACCCAAG AAGAAAGGAAGGGGACCCAAAATTTTGCAGGTAGCATGTCATTGCTTCAGTGCTCTCTCCTTGTCACTAG TCACTTTTAGCATAATCTGGCTGTGAACAACAATAGCCGCCCAAACTCTTTCTTCACTGGTCATTCCATC ACAAATGTCACCCATGTCACCAAGGGGCTGGGTAAAGGAACCCAAGGAGAGGAACAGAACATGAAAACTG AAAATAGAACCTAATTGGCACAAGCCCCCAGTCCCAAAAATCTCACTTTTCATACCTACTCTAAAAAGCA CATGATTATACCCACACGTACATGCACACACACATGCACACAGGCATGCATACACACACACACACACACA CACACACTATTAGATGAGAACATTGAAATGGCTGAGCAACTTCGATTGGAACCACATTGCCCAATCCAAG GCCCATCTTAAATTCCCTGAGCAGTTTGCATGGTTTGAGCTCCTCTCTGAATCCATCTAGTTTCTGCTGC CAGTGTAGAGTCAGTTTGGCCAGATAAGGAGATGGCACTGCCAAGTGATACATGCTACCCGAGTAGCCTG ACCCCTAGGTGTGCTCCTGGGAGGAAAGATCTGGGGGACAACCCCTACCCCAAGCACACCTATGGGCCAT CTCTGTCAATCTCCTGGGGAGCCCCCACTTTTTAGGGGCTCCCCAGGAGACTCACACTGATGTGGGGAGT GTGGGAAGTCTGGCGGTTGGAGGGGTGGGTGGGGGGCAGTGGGGGCTGGGTGGGGGGAAACTATGGGTAG GAAGTGGTCCCAGAGAGGTCTTAGGTGGAACAGTCAGGAGGAGGCACAGGTCAACTTGCAGAATTACTGA AGAATCAGGACCCCAAATTTTATGTCAATTGATCTATTCCCCTCTTTTTATGTCTGGGGCAGGTTTTTTC CTTTTTTTTTTTTAATCCCTCCTTAGCTTTTAATGCGCTCATAATCCCATTCCCTATGTAACGGGGGCAG CGATCAAGTAATGAATGCATCAAGCCATCAATACCAGCGAGAGCCAGTAACACCGGCTAGAGCCATCAAC ACCGGCTTCCACCATGTCCTGCTCCCAACCATTTATCAACCTTTTTTTTTTTTATCTGTCTCTATCGCTT GGCCTGAGTTGGGAGTGGAGTCTCTGTGGGGTGCTGGCCACGCACCCGCAGAGAAATAAAAGGAATTGAG AAGGCCGCTACCTGGCCTGACTTCTGGGGACAGTGGCTGGTCCCCAGAAGTTCTGAGGAGTGGAGGGGGC GTGGGGCAGTGTCCCCTCAGGTGTTAGGAAGGTGCTCGGAGGCCACAAAGATGGGGACCCCAGCTGGCCC TGCCAGTTGGGGGGGAAGGGGATGTAGATGTAAGACTAGAGAGGTTCCATCAGGCGGGAGCAAGTGGCTG CCTTCTGAGCACTTGGGGGAGGTCCTCCCCGTGCCCCTCAGTGTCATCTTGCCCACTCCTCAGCACCCCA TCTTACCCTCAGGAGGTCTGGAGCTCTACAGACCTCCTGGGGGCAAGGTGGGGTGAGGCCTGGAGCTGGG GAAGCGAGGAGGCTTTAAAGCCTTCAGAGCCAGGAGAACTGTGTACATGGGGTTGTCTGGGCCCTGGGGC CCGAGGGTCTGGTGAGCCGTAGCAGCCACTCCACGGTGCCTAGGACTGCGGCGGGGAACAGGGCGGCTGG AGGTTTACCTCACCCCCACTTCTGCTTCCAGTGCAGTCCCCCTGCCCAACAGTCCTACTAGTAATCTAGA GGCCTGAGGCTTCTGGGCCCAGGTGACAGGACTGGCACCACCCTGGGGGCGGTGTGTGTCAGCCAGCCAT GGCACAGAGGGTTCTCAGCAAGTGCCTAAAGAATGGGCCATTTGGAACATTGGACAGAAACTCAAAGAGT AAATTGTTATAATTGGAGAATATGAATTGGCCTGGTACCCAAAATATCTCGAGGCACCCTAAATTACCTG CCCATTTGACTGGACATCCACCCAGTGTTAATATGCCTCGTGGGATGGGTGTTTTCAGGGGCATTTGCTG ACCATCCTCTGTGTCCCCAGATTTGCAGTTCTCCCCATCATAGGTCACCCTGATGCAGGCACCTCCCTGG CCTCCCATGCCTAGTGTGGCCCTCCATCTTGTTTTGTCTCTTCCCTACTGTCTTCGGTGGGATCCCCTCT TGGGTCCCCCAATTTGTCATCCTGTGAAGACTTCCCACGCGTCGAATGCCATATGTCACCTGTGCCACTG CCCATGTCATCCAGCAGTGGCCCCGGGTATTTGCCCCAACTCAGTCCTTTTAACATGCATTTTCTGGCAA AATCCAAAGCTTGGGTTTTGTTTTTAACCTGTTAACGCTTGCAAACCTAATAAAGCATTCAAAATACT >Human CCGCTAATGTACCATGCCCTGGTGCTGGAAAGTGCCTGAGCCAGCTGCCCCAGCGGCCTCAGCACTACCA AGTTGGCACAAAGCTCCCCAAATTCGGAGGGGCTCAGGGAAACGAGTGGAGGGGATGAGGAGGTGAGGGG TAAACCCATCATTTCAGTTGGCATTTGAGCAGGTGCCATGCTCAGCGGAGATGAGGCTCTCCCATCTGTA GGGGCCGTATTAACATGCACACTCTAAAAGTGCCCTTCGTTTCTCCAGCCTCAGCTTTGTCCCTCTCCTC CTCCACGTCAACCTGGCCAGAGGGTCTGGACGCCACAGCCAGGGCACCCCCTGCTTTGGTGGTGACTGCT AATATTGGCCAGGCCGGCGGATCATCGTCCAGGCAGTTTCGGCAGAGAGCCTTGGGCACCAGTGACTCCC CGGTCCTCTTTATCCACTGTCCAGGAGCTGCGGGGACTGCGCAGGGACTAGAGTACAGGTAACTGGGCTC CCATCCCTTGAGTGTGAGAGAAAAGCTGCACTTTAAGTGTTCAGGCGTGGGTGGGCCGGGAGCTTGGGGC AGCTGCCAGGATCCTGGTTTCTGAAGGAGGGGAAGAACTTCTGCTGCTGGAGGGTGCAGGGAAGCCTCCT GAGAGCAGCCTCAACTTCAGGGATGGGGTGTGCAGGAAAGGCCATTGTGGAGAGGGTTCTCCTTTAGGGC TGCACAAAGCCACTGAGGCTTTTGCAAGGAAAATAGGTTTTCCTTGACTAATTCACCAAGCAAAATGGGA GGGGTAGGGGAGGAGGGCTAGGCCGCTCTTCCCAGCGGGAACACACAGCTGTCTTCACAAGTGTGAAAGG AAGAGTCTTTCTGTGTGAAAAGTTTCCTCCCGTTGCATCCCCCATCCCATTCCCAGAGACAAACAGGAGA CTTTGCAGAGGAGCCAGGGGCCCGAGATTCTGGCGCAGAGATTTTATTTATACATATATACACCATTTTA CAGGTAAAGCTTCCTTCCCTCCTGCCTCCCTATGCCTCCTGACCACCAGCAAGAAATTGGACAGGAGACT GAGGAGAAATGCCGGGAGAGGCAACAACCGCCCTCCATGTCCCCCCTAGGTTTAGCTTCTCTCCTCCTGA TGGCGCACCTGGTCCCCCTTGCTGCTCTCCCAGCCTCCCTGGCACAGAGAGGCGCCCTGGGGCCAAGGCA GTTTCCCTGGGAATGCTCATTCATGCATGAAGTTTTTCTCTGTTGCACCCTGGACCCAGACTCCTCGATC CACCCAGGGTGGTGTCTGTGGGGAGGGGGTTCATTTCCCCAGGAAGCACAGCCACGCCGTCCCTCACTGG CCTCGTCAAGCAGAGCTGTGTGTCCAGTGGCTTTTGCTGGGGCCCCCTCCTTATCTCCTTCCAAGGTGGG GGTGTTTGGAGGTGGAGGAGGCTTTCATATTCCGTGCCATGACCCCTCAAGGCGGGCCATTCGTGTGCAC CCTCCACCCCCAGTGCCAGGCAGAAGCCCATCCTCACCCAGGAACAGGGCAGCCTGTCCAACAGAAGGGT CTCGGCCTCTCCATCAGCACCGGGAAGCCCTTTCTAGGCAAACTTCTCACCACTTCTTCCCTCCCTTATA CTTTGAAAGAGGGAGCTCTAGGCAGGGGAGGGGCTAGAGGGGGAAGCCGCTGCCCAGATCCTGACAAGGT GACCTGAAGGAACCCGGGGAGGGGGATGGGACAGGGCTCAGGCTTGGGGTGTATGGGGAGGGGGGCTTTG CTTTTAAAAGAGGTCATCTCAGCAATATCTTTTTGTTTTTCCCCAGGGGCCGAAGAGTCACCACCGAGCT TGTGTGGGAGGAGGTGGATTCCAGCCCCCAGCCCCAGGGCTCTGAATCGCTGCCAGCTCAGCCCCCTGCC CAGCCTGCCCCACAGCCTGAGCCCCAGCAGGCCAGAGAGCCCAGTCCTGAGGTGAGCTGCTGTGGCCTGT GGCCCAGGCGACCCCAGCGCTCCCAGAACTGAGGCTGGCAGCCAGCCCCAGCCTCAGCCCCAACTGCGAG GCAGAGAGGTGAGTGTCTCAGGCACCCTGAGGCCTGGCAGAGAGGGCCACAGGCTCTGCGCGGGAGTCTT CGAACTGGGATCTCCCCCTTCTGCAAGCAGCTTTGGCTCAGAGAGGCTGGCGTGGATTCAGTCACACAGC TGGGATCTGGAGTTCCGTGGTTGGCTCCAGGTGCTTCCGTCTAGGGGCCAGAGCAGGTGTGGGCAGAGCA GGTTCCCCGCAGTCTCCACGGCACCGAGGTCCTGGCAGGGGAGCTCCTGGGAGACGAAAGAGGGCAAAGA AGGGGAGAGGGGCAGGGAGAGAGCGGGCAGCCAAAGGGGAGAAGATGGGGGGCAGAAAGTGGGTAGAGAG GGAAAAAGGGAAAATATCATTGGGGAAGAACCTAAAAACCCAAGGAAAGCTGGGCTCTGCTGGGGGCTGT GAGACCCCCGGGTTCTCCCCGCCCCAGGCTGCTGGCCATGGGGTCTTGCACCAATGGCCTGACCTTTCTG TCGGTCTGTATTTATCAAAGTGGGTGACAGTCTCAGGCCTCCTGGCTGTTCAGAATTGAGGTAATAACCA GAGGCCTTCTGAGCAAAGGGCCTAAGGGGCTCCGGCGTCAGGATCCCATTGTGGTCAGGAGCCTGCGGGG CTTCCCGTGTGCAAGAGGGGTGAAAGGTGGCTAGAAAGGCCCAGCCAGTGGCCTCTGCCTCAGCCAGAGG GAGCTCTGTAGTGGGGGCAGCACCCATTCACTGGTCAGGCACTGGGGTGACAGGGGAGGCTCCAGGACTT GGGGAGCGTTGGAGCTGGAGGCACATGGATTGGAGTCCCTGTACCTGCCCCACGACAGGGCCTGCAGGGA GGGATCCAGCAGGTGACTCTTCAGGCTGATTTGCCCATCCCAGATAGAAGCCGGGAGTGTTCTTTCAAAG GTGTCTTTACCTTAGACACTCAATAAAATGGTAACACAGTGGCGCCGCCTCAGTCCTTTGGAGTGTGCAC CGTCTGAACCCCTCTCCCAGGGCCCTCTCCCAAGCACCCCAACCTGGACCCATATCCCCCACGTACTTTT GGCTTTGGGCAGATTGAGCAGCCTTGGGGTGGTCTGTGCTGTCTGGTGTGGAGGGTTGCAGTTCGGGTCC TTAGTCCTACTTCCCAGGCCGGCCGGGCTGACGCCAGCGAGTGTGTCCTTCCCCAGCGAGGGGAGTGAGC GCAAGGTCAGCGCCTCGTCTGCGGCGCCCTGCAGGGGGTGACGGAGGGGCGCTCTGAGGACCCTTGGAGA AAGGAGCTGGGTTTGTAAAATGCTGGGCTTGGTCCCACGGACGGCGGAGCGGTGAGCTCAGAGCCAGAGC TGGGGAGGAAATGGGAATGAGAAAGGCCCACTTCAGGGCTGGTGAGCGAGGGGATGGGGAGCAGCCACAG GCCGAGGCTGGGGCATGGGCCAGGCTCCATGGGGTGAGTCTGAGTCCTTGAGGGGATGTTCATCCTCTGT GGAATGTGGGTTTGCCAGTGGAGAGGAGACCAGCGTTGCCCTGGTGAGGTGCTGGTTCAGGGCTGGGGGG CGGACGCTGCTTGGGGCTAAAGTTCCTGCCGGCCAAGCTCTGGGTGGGAGGAGACCCTGGCCCCCTCCCA ACACCCTTGGACTGCTGGCGGGACCCTTCCTACCTCCGGGGGCTGGAAGTAGTGGGGGAGGAGCCAGTCT TGAGGAAGAACCCCGATGCTGGTCTTGACTAGAGGGGAGCCGGTGTGCTTTTCGAGCCTCAGGGTGACCC GCGTCTGCCCCAGCCTCCAGCCTGCCCTGGTCACTTCTGACTAAATAAGGAGAGCACTCAGCAGGCAGCC CCACGAGGGAGGGGGAACATGTGTGCACCCCCACTCCCCCACCTGCTCCTCCCTCCCTACAGGGCCACTA CACCCTGCTGTGGGCACCCCAAGGTGACCCTCAGCCTTCTTCCTACCTTAAAAAGTCCAGGCATGCATTT TCAAGCATGAGCGGTGGCCCCCTGGGGGAAGGCACCTCGGCAGGGCAGAACAAAGGGAAGGGACCCCCAA ACAGGTCACTGGTGTAATTGTCCCCAGCACCCCCAAAGAGGAGGAGAACCCACAACTCGGAACTGGGGCT CACCCCCGATGCCCAACCTGTCCCCAGCCTGGGAAGCAGGCGTGGAGGAGAAGGTGGGGGGAGCCTAGAG CTGGCCCTGGGGGCCCTGGTTTTGTCCATGACGGGAGCCTCGGCAACCTAGTCCGCTCTCCCGGGGACCA GGTTTGCAGACAGGCACCTTTCAAATGCTCCTCACCCCCAAATTTACAAGTCACCCTGCAGAGGAAAACA TCAACACAGCCAGGGGTTCTCTGCTGGAGGCTCCCCCTTCTATAGGCACAGCCGGAGAGGCCAGAGAGCT GGGGACACGGGGAGGCTGCAGAAGGCTGGTGGGAAGGGGGGCAGTGATGGGTGGGGAGAGATGGGCCAGA TGTTCTTGGAATGGGACATGGGGGTGATTGATGCAGACAGAAATTTGAAGGGGACATTCCCACGTGTCTT GTTCTGTGGGTGGAAAATGGGCTGTTTTTCATGGTGGGGGCGGGTTCTCCCTGTCTTGCCAAGCTAATGT GAAAGAGATGCCTCATCCTGCCCAGCTCCCCACACCTGTCCAAGGCCATTAACTTCTGCCTCCCCAGTGT CAGGCTTTGAGATGCCCCCCTTCTAGCCGGGGTCCTCCTATGGGGTGACAATGGGGACAAGCAATGCCCA CTGTAGTTGCCCCAGGATCCCCCACCATTCTGCTGGTCCCCAGCGGTGCCCCCTCTCTGGCAGTACCCCC ACCCACCCCACAGGTCCCCTTAGGGCCACTGCCCCATCGCCCGACATTGCCCAACGCCAAGGGGTGACCT TGTTCCTGCCGACAGGGCCGTTGGGCGCCTGCATGCGGGTTTAATATTTGCCTATAAGGAACTGGGCTTT CCCCAGCCGGAGTGGACAGACTTTCCCTGAAAATTCGCTTGGAGAGAACGAAAAGAGACCCCTGGCACCC CAGCGGCGTGCAGCCCTGCACCCCCCTCCTCCCGGGCCCCGTGTTTCTCATTTTCCTCCCCACTTCCTCT GCTCTTCAGTGTTACCCAAACAAAACTGGTTTCACCCTTGTTTGGTGCTGGCGAAGGCCCGAACGGCGCG CGCAAAGCTCCGGGGCAGGCCGGAGGTGGCCACCGGGGGTGCTCCGGGCCCCCAAGCCAAGCCGGGGACT AGCCTGCCCCCGGTGGCGGCTCGGCCGCGGCTTCGCCTAGGCTCGCAGCGCGGAGGCGAGTGGGGCGCAG TGGCGAGGGGGAGCCTGCGGACCTCCCACGCGGGGACCGAGCAGGTATCTGGGAGTCCCGGGAGCGCCCG GGAAGCAGCGTCCTGGTCGCTCCCTCGCGGCCCTTGGGTTTCTTCCTTACACCCGGACGCCCGCTAAGCT CGGGCTGCCGCCACAAACGCGCTCTCCGTGTGGAGAAGGCAAAGAAAAAAAAAAATAAAAGCAAAAGGAA GAAAAACCCCAAAGAACGAAAAGCAGAATTTCAGCCGGCCGTGCGCGCCAGGGCGCTCCGCGCTACCTGC CCGCGCCGCCCGCGCTCGGGTTCCCGGGGAGGGCGCCAGTGCTCCGCGCGCGCCCCAGCCAAGGTGAATC CCCGGCAGCGCCTTCCTTCCGCTGCCCGGGAAGCTTGAGCTCAACAATTAGCCCTTGATCCTCGGGGGAT TCCAATCCACGGAACAACTTCCCTGCTTTCCCCGAACTCGGACATTTTACTTTTTCTGGGATCCTCTAAA TTTAAGCATTGCTTCCCAAGTCTTCTAAATATACTCACCATTTCGACGGGTCACAATAATTTTCTTGGAC GTTAATTTCCGGGGACGTCAAAACACATCAGTCCCGGCGGGCTTTTCCAGACTTACACTATGTGGCCTGG GGCCCCAGATGTGCTTTCTCCCAGGCTCTGGACAGTTTTATACACCCCCTCCAGGTCCCACAGATTTACA GGCCACTAACCCGGCTTCCCTAATTTTAAAGACGAAGCCCTTGGTCCGTGGTGGTCGCGCTGACCAATTT GCCTGGCTCCCCAGGATGTGGACAGTGCCTTTTCCACATTTAGGCATTGTTTCCAAAACAAGTGGAACTT TCCCGCATAATTTTGAATATTAACTCCAGGGTCTCCTAAGCTTACTGTTTCCGGCACACGTCCGCCCCAT TCCGCGCCCCCCCACCCCACCCCCGCGCCCCTTCCCGTTCACCTCAGCATGGGACATTTGCTGTTGGGTC CCGCAAATCTATTCACACTAACCTGGGTTCCCTAAACTTTACACGTTGAATCCCAAGTCCCTCATGACAC TCAGCAGGGCTGGAAGGTTGGAACCCTCAGAGTATGAAAATTGCTTCCCATACCTTCCCCCAAATTCGGT CATTACACCCAGAGCATGTTGAATCCTTTTCTGAATCATAAACGACCTGCCCTCTGATTGCCTGAGTTTC ATAAAATGGAGGGATTTCCCCAGTGATTCCCCAAAGCTATTGAGAATGTTGTGGGTGTGTGAGTCACAAA GCTTTGGGGCATTAACGTCTATGGCTCTATATGCTGCCTGGCCACGAATCAGGTCCCTTAAGATGTAGAC AGTGCCACCCAGGTCCCTGGAGCACACTGAGCAGTTACCAGGAGGTGCTCAAGTGTGACCCAGGATTCTC CAGGTCCCCCCAAATCACACAGGGTCTCCCGGGTCCCTCTGGGCTACACCAAGCACAAAAGGACCCCTTG GGCAGAGCCTACTTTTATTTCTGTTATGCCAGGTGTTGCTAACGGCCCCAGTTCCCAAACATTCCGAGCA CTTTCTCTGCATCACAACATATTGACTATTAAACAATTCTCTGGGTCCCAGGAGCTTCATAACAAGAATC TTGCTTTTCTAAAATTCAGGCATTGGTCTGAAACCCCAACGGCCAGGATCACACTGGACCCTTTTCCTGG TCCCCCATACTTGGATGTTCTGGACGCTGCCCTCCAGGCCCTCTAGGAACATTCAGCATTGCCCTCGGAT CACAGGACAGCACTGATTTCTTGGGCTCCAAACAACCCACTGAGTCATCTCAAAGTTAAGCAATATTTCC TTCAAGCACACTGCACACTCCCTATCACAAAATCTGAAAATTCCTAAGTCCTAAGACCTAGGAATTCTGA ATCCCCTTTCTTTAAAATGTACATATGGACCCCCAAGTCCTCCAAGGACTCTGAGCAACTTCCCTAGATC TTTAGATTCAAAAACGATTTTCCTGGAGCCCCCAAATTGCGGTATTGTCTCCCAGCCTTCCAAAGCAAAT TGAGATTTTTTTCCCTTCACAAAACAATTGAGGTTTTTTTTTTTTTAATACTGATTTATGAGTCTCCTGA CTTTATGGTCCCTGCCCTGGGTCCCCCTACATTTAGAAAATGTTCCATGGACCCCCAAAGCACACTAAAA AATGTCCCTGGGTCCCAAGAAATCCCAGGCATGGAAAAACCTGCGACCTATAAGTTTCCTAGCTACTAAC TAGGTTTCCAGAAATTTAGATATCAAATCTCCATTGGGTAATTTCCATGTGTCCCAAAAACTTGAAATGT GTTTCACTGGGGCTCCCCCAAATGCAGACGACATCCAGGAAAATATATAGTCTTTTTCTTATTTACCAAA AATAAGCTAATGGAAATCATTTAAAAATTAGCATAGAAAAATAATACTGATTTTTTATTTTTTTATTTTT TATTTTGCTTTCCCCAAATGTACTGATCACACTCCAGGCTCCCCCAAAATCTAGACAGTGCTTTCTTCCA TCTCTGAAGGGTGTTAAAACCTTTCCCTGAAGCCACAGTAATTATGAAGGTTATTTTTTCCCCGGCTGCT GCCAGCGTCCAGGCCACTAACTTATATTCTTAAGATGTGAAAATTAATCTCAGCTTCCCCCTAACACACC AAGAATGTGTTTGGATCCCCAAAATGTGTTCCTTGCTTTCATCTGCCAATTTTACGTAATATGGCTCTAC GGCAAAATTCCCAATTTCATATGGAGAATTTTCTTTAACTACCCCTCCTCACAAATTGGTCCCCCAAGCT AGCTGGCCCCTATTTGAGACCTCTTTCTCTATGTTCCCAATTGCATGGAGCAACTTCTCTCATCCCCCAA ACCTGTAATCTATTTTTCTGGAGTCTCGAGTTTAGTCATTAATCACGGTTCCCACATTAACGGAGTCCCC GGGGTCCCCTCCTCCAGGACACCCATTCGCTAAGCCCGCAAGGCAGAAAGAACTCTGCCTTGCGTTCCCC AAAATTTGGGCATTGTTCCCGGCTCGCCGGCCACCCACTGCAGCTTCCCCAACCCCGCGCACAGCGGGCA CTGGTTTCGGGCCTCTCTGTCTCCTACGAAGTCCCCAGAGCAACTCGGATTTGGGAAATTTCTCTCTAGC GTTGCCCAAACACACTTGGGTCGGCCGCGCGCCCTCAGGACGTGGACAGGGAGGGCTTCCCCGTGTCCAG GAAAGCGACCGGGCATTGCCCCCAGTCTCCCCCAAATTTGGGCATTGTCCCCGGGTCTTCCAACGGACTG GGCGTTGCTCCCGGACACTGAGGACTGGCCCCGGGGTCTCGCTCACCTTCAGCAGCGTCCACCGCCTGCC ACAGAGCGTTCGATCGCTCGCTGCCTGAGCTCCTGGTGCGCCCGCGGACGCAGCCTCCAGCTTCGCGGTG AGCTCCCCGCCGCGCCGATCCCCTCCGCCTCTGCGCCCCTGACCGGCTCTCGGCCCGCATCTGCTGCTGT CCCGCCGGTGCTGGCGCTCGTCTCCGGCTGCCGCCGGGGAGGCCGGCGTGGGGCGCGGGACACGGCTGCG GACTTGCGGCTGGCGGCTGCGCTCGCTCCTGCTGGGCGCCCCGAAATCCGCGCCACTTTCGTTTGCTCAT TGCAAAGATCTCATTTGTGGGGAAAGCGGCTGGAGGGTCCCAAAGTGGGGCGGGCAGGGGGCTGGGGCGA GGGACGCGGAGGAGAGGCGCTCCCGCCGGGCGGTAAAGTGCCTCTAGCCCGCGGGCCTAGGACTCCGCCG GGAGGCGCGCGCGGAGCGCGGGCGAAGTGATTGATGGCGGAGCGAGGGGGGCGAGGGGGGCCAGGGGGGC GCGAGATTCCGCCGGCGGCCCCTTCCCCTTGGCTAGGCTTAGGCGGCGGGGGGCTGGCGGGGTGCGGGAT TTTGTGCGTGGTTTTTGACTTGGTAAAAATCACAGTGCTTTCTTACATCGTTCAAACTCTCCAGGAGATG GTTTCCCCAGACCCCCAAATTATCGTGGTGGCCCCCGAGACCGAACTCGCGTCTATGCAAGTCCAACGCA CTGAGGACGGGGTAACCATTATCCAGATATTTTGGGTGGGCCGCAAAGGCGAGCTACTTAGACGCACCCC GGTGAGCTCGGCCATGCAGGTAGGATTTGAGCTGTGTTTCCCGCCCTGATCCTCTCTCCTCTGGCGGCCG GAGCCTCCGTAGGCTCCAAGCCTGGCCCAGATTCGGCCCGGCGCAGCCGGCCTTCCGCGCGTCCCGCACC TGGCGGGGGCTCCGGGGCTCCGGCGCGGCACCGGGGGGCGCTCGGGATCTGGCTGAGGCTCCAAGCGCCG CGTGGCCGGCTCCTCCTGCTGGGGCAGGTGGCGGCTGCGCGCCCCGCCCGAGCCCAGGGGCCCCCTCAGC CGCAACAACCAGCAAGGACCCCCCGACTCAGCCCCAAGCCACCTGCATCTGCACTCAGACGGGGCGCACC CGCAGTGCAGCCTCCTGGTGGGGCGCTGGGAGCCCGCCTGCCCCTGCCTGCCCGGAGACCCCAGCTCACG AGCACAGGCCGCCCGGGCACCCCAGAAACCCGGGATGGGGCCCCTGAATTCTCTAGAACGGGCATTCAGC ATGGCCTTGGCGCTCTGCGGCTCCCTGCCCCCCACCCAGCCTCGCCCCCGCGCACCCCCCAGCCCCTGCG ACCGCCGCCCCCCCCCCGGGGCCCCAGGGCCCCCAGCCCGCACCCCCCGCCCCGCTCTTGGCTCGGGTTG CGGGGGCGGGCCGGGGGCGGGGCGAGGGCTCCGCGGGCGCCCATTGGCGCGGGCGCGAGGCCAGCGAGGC CCGCGCGGGCCCTGGGCCGCGGGCTGGCGCGACTATAAGAGCCGGGCGTGGGCGCCCGCAGTTCGCCTGC TCTCCGGCGGAGCTGCGTGAGGCCCGGCCGGCCCCGGCCCCCCCCTTCCGGCCGCCCCCGCCTCCTGGCC CACGCCTGCCCGCGCTCTGCCCACCAGCGCCTCCATCGGGCAAGGCGGCCCCGCGTCGACGCCGCCCGCT GCCTCGCTGCTGACTCCCGTCCCGGGCGCCGTCCGCGGGGTCGCGCTCCGCCGGGCCTGCGGATTCCCCG CCGCCTCCTCTTCATCTACCTCAACTCCCCCCATCCCCGCTTCGCCCGAGGAGGCGGTTCCCCCCGCAGG CAGTCCGGCTCGCAGGCCGCCGGCGTTGTCACCCCCCCCCGCGCTCCCCCTCCAGCCCTCCCCCCGGCGC GCAGCCTCGGGCCGCTCCCCTTTCCGCGCTGCGTCCCGGAGCGGCCCCGGTGCCGCCACCGCCTGTCCCC CTCCCGAGGCCCGGGCTCGCGACGGCAGAGGGCTCCGTCGGCCCAAACCGAGCTGGGCGCCCGCGGTCCG GGTGCAGCCTCCACTCCGCCCCCCAGTCACCGCCTCCCCCGGCCCCTCGACGTGGCGCCCTTCCCTCCGC TTCTCTGTGCTCCCCGCGCCCCTCTTGGCGTCTGGCCCCGGCCCCCGCTCTTTCTCCCGCAACCTTCCCT TCGCTCCCTCCCGTCCCCCCCAGCTCCTAGCCTCCGACTCCCTCCCCCCCTCACGCCCGCCCTCTCGCCT TCGCCGAACCAAAGTGGATTAATTACACGCTTTCTGTTTCTCTCCGTGCTGTTCTCTCCCGCTGTGCGCC TGCCCGCCTCTCGCTGTCCTCTCTCCCCCTCGCCCTCTCTTCGGCCCCCCCCTTTCACGTTCACTCTGTC TCTCCCACTATCTCTGCCCCCCTCTATCCTTGATACAACAGCTGACCTCATTTCCCGATACCTTTTCCCC CCCGAAAAGTACAACATCTGGCCCGCCCCAGCCCGAAGACAGCCCGTCCTCCCTGGACAATCAGACGAAT TCTCCCCCCCCCCCCAAAAAAAAGCCATCCCCCCGCTCTGCCCCGTCGCACATTCGGCCCCCGCGACTCG GCCAGAGCGGCGCTGGCAGAGGAGTGTCCGGCAGGAGGGCCAACGCCCGCTGTTCGGTTTGCGACACGCA GCAGGGAGGTGGGCGGCAGCGTCGCCGGCTTCCAGGTAAGCGGCGTGTGCGGGCCGGGCCGGGGCCGGGG CTGGGGCGGCGCGGGCTTGCGCCGGACGCCCGGCCCTTCCTCCGCCCGCTCCCGGCCCGGGGCCTGCGGG GCTCGGCGGGGCGGCTGAGCCCGGGGGGGAGGAGGAGGAGGAGGAGGAGGACGGACGGCTGCGGGTCCCG TTCCCTGCGCGGAGCCCCGCGCTCACCCTGGCGGCGGAGCTGGGGGTGGGGTGGGGGCGTCGGGAAGGGC CGAGGGAGGTGTGAGGTGTCTGCAGGGGCGACTTCCCGGTCGGTCTGTGGGTGCAGGGGGTGCCGCCTCA CATGTGTGATTCGTGCCTTGCGGGCCCTGGCCTCCGGGGTGCTGGGTAACGAGGAGGGGCGCGGAGCCGC AGAAGCCCACCCTGGTATGTTGACGCGGTGCCAGCGAGACCGCGAGAGGAAGACGGGGGTGGGCGGGGCC AGGATGGAGAGGGGCCGAGTTGGCAGGAGTCATGGCAGACGCCACATTCGCGACATCTCCCCCACACCCC CTCTGGCTCTGTCCGCAACATTTCCAAACAGGAGTCCCGGGAGAGGGGGAGAGGGGCTGCTGGTCTGAGG CTAAGAAGGGCAGAGCCTTCGACCCGGAGAGAGGCCGCGGCCCCTGCCCAGTGGGCAGCGTGGAAGTTTC CATACAAGGAGGTGGGAAGGAGACCCCCCCCCCCTTCACTGCCCTGTGCAGAGATGAGCCGGGGGTGCAG GATGGGAGCCCATGGCACTTCGCTACGGGATGGTCCAGGGCTCCCGGTTGGGGGTGCAGGAGAGAAGAGA CTGGCTGGGAGGAGGGAGAGGGCGGGAGCAAAGGCGCGGGGGAGTGGTCAGCAGGGAGAGGGGTGGGGGG TAGGGTGGAGCCCGGGCTGGGAGGAGTCGGCTCACACATAAAAGCTGAGGCACTGACCAGCCTGCAAACT GGACATTAGCTTCTCCTGTGAAAGAGACTTCCAGCTTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTGCC CCAGCGAGCCTTCTGCTGAGCTGTAGGTAACCAGGGCTGTGGAGTGAGGACCCCCGCTGCCATCCCACTC CAGCCTGAGGCAGGGCAGCAGGGGGCACGGCCCACGCCTGGGCCTCGGGCCCTGCAGCCGCCAGCCCGCT GCCTCTCGGACAGCACCCCCCTCCCCTCTTTTCCTCTGCCCCTGCCCCCACCTGGTCTCTGCTCCCTCAC CTGCTCCTTCCCTTTCTGTTCCTTCCCTTCGGCCCCCTCCTTGCCCAGCTCAGGACTTTTCCTGGGCCCT CACCTGCTCCGCACCGCTGCATGCTTCCTGTCCTGCTTTCTGCCGGTCCCCTGACCCGGACCTCCAAGTT CAGAGTGGTGGGGCTTGTTGCGGAAGCGCGGCGAGGGCTAGAGTGGCCAGCTGGCGGAGTGTGCTCTTAG AATTTGGAAGGGGGTGGCAGAGGGGGCGGTGAGAGGACTGGCCAGGGTCCAGTCAAGGAGATGACCAAGG AGGCTTTCAGATCCTCGGCGCAGCTGCCCACTAGTCTTTAGAGAGGGCATGCAAAGTTGTGCTTCTGTCC CACTGCCTGCTCAGTCGCTCACATAATTTATTGCATCAAAAACTCCCCTGGGTCTGCGGAGCGAAGGCTG GGGCTGCCCGCCTGGAGGGTTCCACCTTCTGCAGGGGCAGGGCCAACTTGCTGTGGTGGCTCCCGGCCTC CCACCCCCGAGTGGGTTAACCCGGCCCTGTGGCCCTGCAGCCTGTGGAGGGGGTGTGTCCTAAGACTGGC CTCCCCTTCCAGATTGTAGTCTGGGGAACCTGGTGTCGGACTTCCCAGGTGGCCTGAGCTGGTCTCTCCA GCTCCCACGGGGAGAGTTTGGTAGCGCAAATAGGGAGATGTTCTGGAGGCCCCTGGCCTTACTGGTTCGA TTTGAGGCCTGGAAAGGAGGCTCTGGGCGTGTGTGTGTGTGTTTGGGGGTACCCAAGGCAGACTGGAGTT GGAGAACTGGGTGACTGGGAAAACAAGGTTTCTAGAGCATGGGTGGCGTGGTTGTGTTAACCATTGGAGT CCTTGACCCAGGCCTGGCTCAGCTGCAGACTGGAAAGGTGGAAAAGCCAGGGGGAGGGGCGGGGCTGGCC CAGCAGGACTGGCCTGCTGCTTTGAGGGCGATGGTCCTCCTGGACCCCCCCTGCTCAGCTGGGGGTTGTG GGGAGGAAGGGACTGGTCCTCCTGGATGCACATGCTCTGTAGGGGTGGGGCTGTCTGCCATCTTGGCTGG CGCTGGAGGCCTGAGAAGTGGCGATGTGACGCTGGGCTGGCCCTGCCCCCATGGTGTCATAGGACGGAGG CCAGGTCGGGTGTCCAGCCTGGGCCCCTGCAGCTGTGGATGCCGCTGAGCTCCTGCAATAATGACCGTGG AGATGGTCACCCCTCGTGTAAAATTACTAGTGCTTCTTGCAAATGGAAGGAACTGGGCCTTTTCTGTGTG CTTCTGGACGCTTCATTCTGCACATGGCCCTGCGCCCTCACCTCGGCATTATGACCTGTGTGTTACTTTT GTAATAAAAATAATGTTTATAGGAAAGCCGTGCTTTCAATTTTCAACTGAATTTGTAGGTTGGCAAATTT GGTTTGGGAGGGGCACCTCTGGCCTGGGGCTTGGCCTGGCTGCCCCGCTCACGCCACTTCTCTCCCGCCC CCAGACACCAATGGGAATCCCAATGGGGAAGTCGATGCTGGTGCTTCTCACCTTCTTGGCCTTCGCCTCG TGCTGCATTGCTGCTTACCGCCCCAGTGAGACCCTGTGCGGCGGGGAGCTGGTGGACACCCTCCAGTTCG TCTGTGGGGACCGCGGCTTCTACTTCAGTAAGTAGCTGGGAGGGGCTTCCTCAGACCTGGTCAGGCCCCT AGAGTGACCGGTGAGGACGCCCAACCTCAAGCCAGGGGAGCACACTCCTAGGTCAGCAGCCCAGCCGCTT GCTCTGAGACTTTGACCTTCCCGCCGCGTTTCTGAGCACGTGCGGTGTCCCAGGGCATCCACACCAGCTG CCTTTCCCATCACACGCCTCCTTCGAAGGGTGGGCCAGAGGTGCCCCCTAGACGTCAGGGGCACTCACAG GGGTCTCCCTGGGCATCAGAATTTCTGTTGGGGGCCGTGAGGCTCCTGCTCCTGAGGCACCGCACGCCTA GTGCAGGGCTTCAGGCTCTGGAGGAAGAGCCTGCCTTTCTTCCTGCACCTTTTGGACATTCTGACAAGGG ACGTGCGTTCGGTGAATGATCAGAATTAAAATCAATAAAGTGATTTATATAATTAAAATCAATAAGACAA GTGCAGTTGGTGGGTGGCAGGGGTGAGCGGTGCATGCGCCTCCTTGGGCCCCAAGGCTGCCGTGGGGGGT GCCCACCTGCTGACCTCAAGGACGCTTCAGCCTTTCCTCATGTTTCTCTCTTGGTTCTCCAGCCTGGGGG CTGGCAGGTGGGTGCATGGCCCATTGTCCTTGAGACCCCACCCCCAGATAGGGGGGCTGGGTGGATGCAG AGGCAGGCATGGTGCCTGGGCATGCCTGATGGGGCAGGGGAGGGGCCGCTCCTTACTGGCAGAGGCCGCA CGGCTTATTCCACCTGACACTCACCACGTGACATCTTTACCACCACTGCTTACTCACGCTGTGAAATGGG CTCACAGGATGCAAATGCACTTCAAAGCTTCTCTCTGAAAAGTTCCTGCTGCTTGACTCTGGAAGCCCCT GCCCGCCCTGGCCTCTCCTGTGCCCTCTCTCTTGCCTGCCCCATTTGGGGGTAGGAAGTGGCACTGCAGG GCCTGGTGCCAGCCAGTCCTTGCCCAGGGAGAAGCTTCCCTGCACCAGGCTTTCCTGAGAGGAGGGGAGG GCCAAGCCCCCACTTGGGGGACCCCCGTGATGGGGCTCCTGCTCCCTCCTCCGGCTGATGGCACCTGCCC TTTGGCACCCCAAGGTGGAGCCCCCAGCGACCTTCCCCTTCCAGCTGAGCATTGCTGTGGGGGAGAGGGG GAAGACGGGAGGAAAGAAGGGAGTGGTTCCATCACGCCTCCTCACTCCTCTCCTCCCGTCTTCTCCTCTC CTGCCCTTGTCTCCCTGTCTCAGCAGCTCCAGGGGTGGTGTGGGCCCCTCCAGCCTCCTAGGTGGTGCCA GGCCAGAGTCCAAGCTCAGGGACAGCAGTCCCTCCTGTGGGGGCCCCTGAACTGGGCTCACATCCCACAC ATTTTCCAAACCACTCCCATTGTGAGCCTTTGGTCCTGGTGGTGTCCCTCTGGTTGTGGGACCAAGAGCT TGTGCCCATTTTTCATCTGAGGAAGGAGGCAGCAGAGGCCACGGGCTGGTCTGGGTCCCACTCACCTCCC CTCTCACCTCTCTTCTTCCTGGGACGCCTCTGCCTGCCAGCTCTCACTTCCCTCCCCTGACCCGCAGGGT GGCTGCGTCCTTCCAGGGCCTGGCCTGAGGGCAGGGGTGGTTTGCTCCCCCTTCAGCCTCCGGGGGCTGG GGTCAGTGCGGTGCTAACACGGCTCTCTCTGTGCTGTGGGACTTCCAGGCAGGCCCGCAAGCCGTGTGAG CCGTCGCAGCCGTGGCATCGTTGAGGAGTGCTGTTTCCGCAGCTGTGACCTGGCCCTCCTGGAGACGTAC TGTGCTACCCCCGCCAAGTCCGAGAGGGACGTGTCGACCCCTCCGACCGTGCTTCCGGTGAGGGTCCTGG GCCCCTTTCCCACTCTCTAGAGACAGAGAAATAGGGCTTCGGGCGCCCAGCGTTTCCTGTGGCCTCTGGG ACCTCTTGGCCAGGGACAAGGACCCGTGACTTCCTTGCTTGCTGTGTGGCCCGGGAGCAGCTCAGACGCT GGCTCCTTCTGTCCCTCTGCCCGTGGACATTAGCTCAAGTCACTGATCAGTCACAGGGGTGGCCTGTCAG GTCAGGCGGGCGGCTCAGGCGGAAGAGCGTGGAGAGCAGGCACCTGCTGACCAGCCCCTTCCCCTCCCAG GACAACTTCCCCAGATACCCCGTGGGCAAGTTCTTCCAATATGACACCTGGAAGCAGTCCACCCAGCGCC TGCGCAGGGGCCTGCCTGCCCTCCTGCGTGCCCGCCGGGGTCACGTGCTCGCCAAGGAGCTCGAGGCGTT CAGGGAGGCCAAACGTCACCGTCCCCTGATTGCTCTACCCACCCAAGACCCCGCCCACGGGGGCGCCCCC CCAGAGATGGCCAGCAATCGGAAGTGAGCAAAACTGCCGCAAGTCTGCAGCCCGGCGCCACCATCCTGCA GCCTCCTCCTGACCACGGACGTTTCCATCAGGTTCCATCCCGAAAATCTCTCGGTTCCACGTCCCCCTGG GGCTTCTCCTGACCCAGTCCCCGTGCCCCGCCTCCCCGAAACAGGCTACTCTCCTCGGCCCCCTCCATCG GGCTGAGGAAGCACAGCAGCATCTTCAAACATGTACAAAATCGATTGGCTTTAAACACCCTTCACATACC CTCCCCCCAAATTATCCCCAATTATCCCCACACATAAAAAATCAAAACATTAAACTAACCCCCTTCCCCC CCCCCCACAACAACCCTCTTAAAACTAATTGGCTTTTTAGAAACACCCCACAAAAGCTCAGAAATTGGCT TTAAAAAAAACAACCACCAAAAAAAATCAATTGGCTAAAAAAAAAAAGTATTAAAAACGAATTGGCTGAG AAACAATTGGCAAAATAAAGGAATTTGGCACTCCCCACCCCCCTCTTTCTCTTCTCCCTTGGACTTTGAG TCAAATTGGCCTGGACTTGAGTCCCTGAACCAGCAAAGAGAAAAGAAGGACCCCAGAAATCACAGGTGGG CACGTCGCTGCTACCGCCATCTCCCTTCTCACGGGAATTTTCAGGGTAAACTGGCCATCCGAAAATAGCA ACAACCCAGACTGGCTCCTCACTCCCTTTTCCATCACTAAAAATCACAGAGCAGTCAGAGGGACCCAGTA AGACCAAAGGAGGGGAGGACAGAGCATGAAAACCAAAATCCATGCAAATGAAATGTAATTGGCACGACCC TCACCCCCAAATCTTACATCTCAATTCCCATCCTAAAAAGCACTCATACTTTATGCATCCCCGCAGCTAC ACACACACAACACACAGCACACGCATGAACACAGCACACACACGAGCACAGCACACACACAAACGCACAG CACACACAGCACACAGATGAGCACACAGCACACACACAAACGCACAGCACACACACGCACACACATGCAC ACACAGCACACAAACGCACGGCACACACACGCACACACATGCACACACAGCACACACACAAACGCACAGC ACACACAAACGCACAGCACACACGCACACACAGCACACACACGAGCACACAGCACACAAACGCACAGCAC ACGCACACACATGCACACACAGCACACACACTAGCACACAGCACACACACAAAGACACAGCACACACATG CACACACAGCACACACACGCGAACACAGCACACACGAACACAGCACACACAGCACACACACAAACACAGC ACACACATGCACACAGCACACGCACACACAGCACACACATGAACACAGCACACAGCACACACATGCACAC ACAGCACACACGCATGCACAGCACACATGAACACAGCACACACACAAACACACAGCACACACATGCACAC ACAGCACACACACTCATGCGCAGCACATACATGAACACAGCTCACAGCACACAAACACGCAGCACACACG TTGCACACGCAAGCACCCACCTGCACACACACATGCGCACACACACGCACACCCCCACAAAATTGGATGA AAACAATAAGCATATCTAAGCAACTACGATATCTGTATGGATCAGGCCAAAGTCCCGCTAAGATTCTCCA ATGTTTTCATGGTCTGAGCCCCGCTCCTGTTCCCATCTCCACTGCCCCTCGGCCCTGTCTGTGCCCTGCC TCTCAGAGGAGGGGGCTCAGATGGTGCGGCCTGAGTGTGCGGCCGGCGGCATTTGGGATACACCCGTAGG GTGGGCGGGGTGTGTCCCAGGCCTAATTCCATCTTTCCACCATGACAGAGATGCCCTTGTGAGGCTGGCC TCCTTGGCGCCTGTCCCCACGGCCCCCGCAGCGTGAGCCACGATGCTCCCCATACCCCACCCATTCCCGA TACACCTTACTTACTGTGTGTTGGCCCAGCCAGAGTGAGGAAGGAGTTTGGCCACATTGGAGATGGCGGT AGCTGAGCAGACATGCCCCCACGAGTAGCCTGACTCCCTGGTGTGCTCCTGGAAGGAAGATCTTGGGGAC CCCCCCACCGGAGCACACCTAGGGATCATCTTTGCCCGTCTCCTGGGGACCCCCCAAGAAATGTGGAGTC CTCGGGGGCCGTGCACTGATGCGGGGAGTGTGGGAAGTCTGGCGGTTGGAGGGGTGGGTGGGGGGCAGTG GGGGCTGGGCGGGGGGAGTTCTGGGGTAGGAAGTGGTCCCGGGAGATTTTGGATGGAAAAGTCAGGAGGA TTGACAGCAGACTTGCAGAATTACATAGAGAAATTAGGAACCCCCAAATTTCATGTCAATTGATCTATTC CCCCTCTTTGTTTCTTGGGGCATTTTTCCTTTTTTTTTTTTTTTTGTTTTTTTTTTACCCCTCCTTAGCT TTATGCGCTCAGAAACCAAATTAAACCCCCCCCCCATGTAACAGGGGGGCAGTGACAAAAGCAAGAACGC ACGAAGCCAGCCTGGAGACCACCACGTCCTGCCCCCCGCCATTTATCGCCCTGATTGGATTTTGTTTTTC ATCTGTCCCTGTTGCTTGGGTTGAGTTGAGGGTGGAGCCTCCTGGGGGGCACTGGCCACTGAGCCCCCTT GGAGAAGTCAGAGGGGAGTGGAGAAGGCCACTGTCCGGCCTGGCTTCTGGGGACAGTGGCTGGTCCCCAG AAGTCCTGAGGGCGGAGGGGGGGGTTGGGCAGGGTCTCCTCAGGTGTCAGGAGGGTGCTCGGAGGCCACA GGAGGGGGCTCCTGGCTGGCCTGAGGCTGGCCGGAGGGGAAGGGGCTAGCAGGTGTGTAAACAGAGGGTT CCATCAGGCTGGGGCAGGGTGGCCGCCTTCCGCACACTTGAGGAACCCTCCCCTCTCCCTCGGTGACATC TTGCCCGCCCCTCAGCACCCTGCCTTGTCTCCAGGAGGTCCGAAGCTCTGTGGGACCTCTTGGGGGCAAG GTGGGGTGAGGCCGGGGAGTAGGGAGGTCAGGCGGGTCTGAGCCCACAGAGCAGGAGAGCTGCCAGGTCT GCCCATCGACCAGGTTGCTTGGGCCCCGGAGCCCACGGGTCTGGTGATGCCATAGCAGCCACCACCGCGG CGCCTAGGGCTGCGGCAGGGACTCGGCCTCTGGGAGGTTTACCTCGCCCCCACTTGTGCCCCCAGCTCAG CCCCCCTGCACGCAGCCCGACTAGCAGTCTAGAGGCCTGAGGCTTCTGGGTCCTGGTGACGGGGCTGGCA TGACCCCGGGGGTCGTCCATGCCAGTCCGCCTCAGTCGCAGAGGGTCCCTCGGCAAGCGCCCTGTGAGTG GGCCATTCGGAACATTGGACAGAAGCCCAAAGAGCCAAATTGTCACAATTGTGGAACCCACATTGGCCTG AGATCCAAAACGCTTCGAGGCACCCCAAATTACCTGCCCATTCGTCAGGACACCCACCCACCCAGTGTTA TATTCTGCCTCGCCGGAGTGGGTGTTCCCGGGGGCACTTGCCGACCAGCCCCTTGCGTCCCCAGGTTTGC AGCTCTCCCCTGGGCCACTAACCATCCTGGCCCGGGCTGCCTGTCTGACCTCCGTGCCTAGTCGTGGCTC TCCATCTTGTCTCCTCCCCGTGTCCCCAATGTCTTCAGTGGGGGGCCCCCTCTTGGGTCCCCTCCTCTGC CATCACCTGAAGACCCCCACGCCAAACACTGAATGTCACCTGTGCCTGCCGCCTCGGTCCACCTTGCGGC CCGTGTTTGACTCAACTCAACTCCTTTAACGCTAATATTTCCGGCAAAATCCCATGCTTGGGTTTTGTCT TTAACCTTGTAACGCTTGCAATCCCAATAAAGCATTAAAAGTCATGA