Biology Lab Report

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Online Lab 3: Demonstration of Enzyme Activity at Home Normal cellular metabolism can produce reactive species that have the potential to be harmful to the cell. These reactive species can alter the shape, structure, and function of key macromolecules that the cell requires to survive, including proteins, nucleic acids, and lipids. Fortunately, cells also produce protective enzymes that can detoxify these reactive species. One such protective enzyme is catalase. Catalase detoxifies the reactive species hydrogen peroxide by converting it into oxygen gas and water. Materials 3% hydrogen peroxide solution from grocery or drug store (also readily available online) ENZYME ACTIVITY LAB 3 Raw, unpeeled potato at room temperature Knife and cutting board Clear glass cups or small canning or spice jars (at least 5, preferably the same size) antwolle was all reve Water Methods 01 boon woy bine's c. Sharpie or blue tape, for labeling containers Freezer, such as found in a typical kitchen Method to cook potato (may be a microwave or kitchen cook top) Please read before proceeding: 3% hydrogen peroxide is a commonly available solution sold as a first aid treatment for minor cuts and scrapes and as an oral rinse for minor mouth irritation. Do not use hydrogen peroxide solution that exceeds 3%. Do not ingest hydrogen peroxide or get it in your eyes. Read the warning label on the bottle and obey all instructions. Proceed with the follow- ing section only if safe to do so in your home. terroilasap rossos apoy entmaps] dan579010 B12 fooleatozen trià bloorle 13 allop: Section 1. Determine if the Enzyme Catalase Is Present in Potato 1. Use your knowledge of the scientific process and enzyme activity to develop an experiment that will determine if the enzyme catalase is present in raw potato. Your instructor will inform you if you should work individually or with an assigned group. You might need to review the earlier sections of this lab, especially this page. 2. Review and write down your answers to the following: BRIGITA 2.100 a. Determine your research question. What do you intend to learn from your experiment? b. State your research hypothesis. What should be the answer to your question and what should the results look like? LIZOT sq maml buda State your research methods. Explain how the experiment will provide answers to your question. Include the methods of the experiment and provide enough detail so that your classmates can repeat your experiment. BIOL 105 39LAB 3 ENZYME ACTIVITY 3. 4. d. State your predicted results. Describe what you expect to see after your experiments, will conclude if your hypothesis is correct. how you your data, and how will collect you 3. Present the answers to # 2 above to your lab instructor and obtain approval to begin your experiment. 4. Begin the experiment. Be sure to document your experiment with photos to include in your report. 40 BIOL 105 Section 2. Determine the Effect of Temperature on the Function of the Enzyme Catalase 1. Based on your results in section 1, predict what would occur if you heat or cool the potato be- fore performing your experiment. Would you need to alter any procedures or controls in your experiment? nos, Heime to zquo sign પછી તો એ 2. Write down the answers to following: 40 a. Determine your research question. What do you intend to learn from your experiment? 14 2015 b. State your research hypothesis. What should be the answer to your question and what should the results look like? C. State your research methods. Explain how the experiment will provide answers to your question. Include the methods of the experiment and provide enough detail so that your classmates can repeat your experiment. 109 seconbyil d. State your predicted results. Describe what you expect to see after your experiments, how you will collect your data, and how you will conclude whether your hypothesis is correct. JOL A Few Tips for Your Experiments Diced potatoes often work best for this experiment. Present your revised experiment to your lab instructor for approval. Begin the experiment. Be sure to document your experiment with photos to include in your report. ng 10700 assblow billi and abod Even with the help of enzymes, chemical reactions don't happen in large-enough quantities for us to instantly notice them; therefore, it is wise to wait 5-10 minutes before recording your results. Make sure you have CONTROLS for your experiment! 2011201 Cleanup Be sure to throw away the potato scraps and rinse all the glassware used for your experiments thor- oughly with water. Wipe down all surfaces and make sure to cap your hydrogen peroxide solution tightly when you are not actively using it. odwor ous strong ne
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