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Genetic Problem Set Week 9
1. Below is an mRNA transcript encoding a small gene. Translate this gene into amino
acids using the table. Be sure to include the amino and carboxyl ends of the peptide (it is
not necessary to draw amino acid or bond structure, three letter codes for amino acids are
fine). Use the table on pg 310 in your textbook.
5' GCAUGGCUCAUUGUCGGGGGUGAAUAA 3'
A. What would the double-stranded DNA molecule look like that this mRNA
message was created from? Be sure to include hydrogen bonding as well as
indicate which strand is the template and non-template.
2. Describe how Beadle and Tatum experimentally defined 'one gene, one enzyme'.
3. Using the non-template strand of DNA below answer the following questions
5'AGCATCAGCATAATGGGGAATCCTTAAGTA 3'
a. Identify what the template stand of DNA would be
b. Identify the mRNA message that would be created from this particular
DNA molecule
c. Using the mRNA message above, translate into a protein.
DU
U
UUU
UUC Phe
UUA].
UUG Leu
Second letter
UCU
UCC
UCA
A
G
UAU
UGU
Ser UAC Tyr
UGC Cys
UAA Stop UGA Stop A
STAG UTAG STAG STAG
UCG
UAG Stop UGG Trp
G
CUU
CCU
CUC
CCC
CAU
CAC His
CGU
Leu
Pro
CUA
CCA
CAA
CGC
CGA
Arg
Gln
CUG
CCG
CAG
CGG
AUU
ACU
AAU
AGU
AUC lle ACC
AAC
Asn
Ser
AGC
Thr
AUA
AUG Met ACG
ACA
ААА
AGA 1
AAG Lys
AGG Arg
GUU
GCU
GAU
GUC
GCC
GAC
GGU
Asp GGC
Val
Ala
GUA
GUG
GCA
Gly
GAA
GGA
GCG
GAG Glu
GGG
Third letter
d. Change the DNA sequence to induce a mutation within the protein
sequence.
A
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