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Running head: Lab report
The objectives of this experiment are to improve our understanding in relation to how
SDS-PAGE works with proteins so as to be able to determine the molecular weight of protein.
Additionally, in this experiment we will investigate the Purification as well as Size
Determination of Blue & Green Fluorescent Proteins.
In biochemistry, SDS- PAGE in is very essential. This is so since it enables scientists to
be able to separate proteins by their size. This is essential since, whenever bacteria is being
utilized to come up with proteins which are medicinally useful by transformation, then protein
which is of interest should be separated from other cellular proteins. One advantage SDS- PAGE
electrophoresis has over other methods of determining molecular weight of protein is that, it does
not require expensive equipment. SDS- PAGE comprise of 2 essential parts. This are Sodium
Dodecyl Sulfate (SDS) & Polyacrylamide Gel Electrophoresis (PAGE).
SDS is an anionic detergent with negative molecules which can dissolve molecules which
are hydrophobic in nature. SDS usually denatures secondary as well as non-disulfide- linked
tertiary structures. Whenever Proteins are dissolved in SDS, their structure changes from their
usual coiled up secondary, quaternary structure on to a primary structure which is usually flat &