The stain was not produced there, the mitochondria membrane is potential to let the stain inside it. There are dyes which are non fluorescent until it gets through the membrane of mitochondria.
The localization of a substance is because it was formed due to the effect of the dye used in staining,some stains are insensitive and some are sensitive to the cell which can make form some swelling substances .
May 14th, 2015
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