NOTE: This lab assignment has questions related to binomial nomenclature. Please note that the assessment system does not accept formatted text, so you will need to indicate any italicized words by adding “IT” in parentheses after each word that is to be written in italics, (IT). For example, the common fruit fly is genus Drosphila and species melanogaster. When written, it is Drosophila melanogaster. From this example, the correct way to represent the italicized words is by typing, Drosphila (IT) melanogaster (IT). LABORATORY EXERCISE QUESTIONS 1.Part I: You are given a broth culture that is growing an unknown organism. (4 points)a.What is the first test that you will do? Why? (1 point)b.The following image is a result of the test you started in part a. What is the result of this test? Would the catalase test be a helpful test for determining your unknown? What would the test help differentiate? (1 points) c.The following images are a result of the catalase test performed on your unknown. What is the result of the catalase test? Based on the results of the first two tests, what is the genus of your unknown organism? (1 point) d.The following images are growth on sheep blood agar from the unknown organism. Based on all of the results from the unknown organism, what is the genus and species of your unknown? Explain. Differentiate the types of hemolysis that can occur on sheep blood agar and give an example of an organism for each type of hemolysis. (1 point)2.Part II: You are given a broth culture with an unknown organism. (4 points)a.The following image is the gram stain of your unknown organism. What is the result of the gram stain? You need to determine if this organism is a streptococcus or a staphylococcus. What is one test that can quickly distinguish between these two genus of bacteria? (1 point) b.The following images are the result of the test. What is the result of the test? What is the genus of your unknown organism? (1 point)c.You think that your unknown organism does not ferment mannitol. There are two tests that you can perform to determine if your unknown organism ferments mannitol. What are the two tests? (1 point)d.The following image is the result of one of the tests you identified in part c. What is the result of this test? Based on all your results, what is the genus and the species of this unknown organism? (1 point)3.Part III: You are given some wound material from a patient with a foot wound. (4 points) a.The following image is a gram stain of the organism found in the foot wound. What is the result of the gram stain? Based on the results of the stain, there are two organisms that can cause a skin wound. What are these two organisms? (1 point)b.Do you need to know if this organism grows anaerobically? If so, why? How would you determine if this organism grows anaerobically? (1 point)c.Another test that can be used to help identify this unknown organism is the catalase test. The following image is a catalase test from the organism found in the foot wound. What is the result of the catalase test? (1 point)d.This organism grows well on sheep blood agar. The following images are growth on the unknown organism on sheep blood agar. What is the result of the growth of the unknown organism on sheep blood agar? Based on your results, what is the genus and species of the unknown organism? (1 point)4.Part IV: Assume that you are given a broth culture with an unknown organism. (5 points)a.The following image is a gram stain of the unknown organism. What is the result of the gram stain? (1 point)b.The following image depicts growth of the unknown organism on sheep blood agar. What is the result of the growth of the unknown organism on sheep blood agar? What are three other types of media that would help in identifying this organism? Explain. (1 point) c.This unknown organism was grown on MacConkey agar. The following image is the growth of the unknown organism on MacConkey agar. What is the result of the growth of the unknown organism on MacConkey agar? (1 point) d.The oxidase test is also useful in identifying the unknown organism. The following images are the result of the oxidase test with the unknown organism. What is the result of the oxidase test? What is the genus and species of the unknown organism? (1 point) e.What is the medical significance of the unknown organism? (1 point)5.Part V: Assume you are given a broth culture with an unknown organism. (5 points)a.The following image is a gram stain of the unknown organism. What is the result of the gram stain? (1 point)b.The following image is growth of the unknown organism on sheep blood agar. What is the result of the growth of the unknown organism on sheep blood agar? (1 point) c.The following images are growth of the unknown organism on MacConkey agar, EMB agar, and XLD agar. What are the results of the growth on MacConkey agar (1), EMB agar (2), and XLD agar (3)? (1 point) d.The indole test is also useful in identifying the unknown organism. The following image is the result of the indole test with the unknown organism. What is the result of the indole test for the unknown organism? What is the genus and species of the unknown organism? (1 point) e. What would the results for all of the IMViC tests be for this organism? (1 point)6.Part VI (4 points)a.An organism grows clear and colorless on MacConkey agar and red with black centers on XLD agar. What is the genus and species of this organism? (1 point)b.What does the gram stain look like for this organism? (1 point)c.What are the IMViC reactions for this organism? (2 points)7.Part VII: Assume you are given a broth culture with an unknown organism. (4 points)a.The following image is a gram stain of the unknown organism. What is the result of the gram stain? (1 point) b.The oxidase test and the catalase test are also useful in identifying the unknown organism. The following images are the results of the oxidase test and catalase test for the unknown organism. What is the result of the oxidase test and the result of the catalase test? (1 point) c.Carbohydrate fermentation can also help identify this unknown organism. The following images are results from maltase, lactose, and glucose fermentation tests. What is the result of the maltose fermentation test (1)? What is the result of the lactose fermentation test (2)? What is the result of the glucose fermentation test (3)? (1 point) d.What is the genus and species of the unknown organism? (1 point)

After reading both websites below, you should be able to see how the discovery of sorting signals paved the way for utilizing these signals to provide information on the localization and functionality of proteins. Propose an experiment for localizing a protein (of your choice) to an alternative cellular compartment. Then, speculate what characteristics or symptoms this would create on a cellular, tissue/organ, and organismal level. Lastly, comment on how this level of organization and exacting localization demonstrates God’s wonderful design in creation (Psalm 139).APA style referenceWebsites:1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC21116...2. https://www.jlr.org/article/S0022-2275(20)31255-4/...