Cellular Respiration Lab Report

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Lab experiment

The rate of carbon dioxide production in organisms
Student name
Lab partners
Course title
Lab sections
Instructor’s name
4/12/2018

Lab experiment

ABSTRACT
The objective of the experiment is to determine the metabolic rate of different organisms. The
experiment uses titration method to determine the amount of carbon dioxide produced by
different organisms. The results indicate that the rate of carbon dioxide production varies
according to the organism mass. This indicates that the lower the mass of an organism, the higher
the metabolic rate of the organism.
INTRODUCTION
Cellular respiration is an aerobic process (doesn’t need oxygen) in which glucose is broken down
into carbon dioxide and water. (manual) During the process, chemical energy is generated. This
chemical energy generated by this breakdown is captured in the form of adenoside triphosphate
(ATP), the energy needed. (Pfeiffer, 2001)This process takes place in animals to produce energy
needed. The source of the glucose is mostly from diet or reserves. (Pfeiffer, 2001)
The equation is:
C6H12O6 + 6O2 -> 6CO2 + 6H20 + ATP + Heat
In aerobic respiration, carbon is broken down to form carbon dioxide. For efficiency of the
system, there is need for quick removal of the carbon dioxide gas produced. When this gas
accumulates, the organism’s environment becomes acidic and several biochemical processes
cannot take place successfully. (manual) Plants utilize the carbon dioxide produced by other
organisms to make its own food molecules. This process takes place in the mitochondria of
different organisms. In plants, for example, this process takes place in the presence of light that
is trapped by chlorophyll, which is found within the chloroplasts. (Lambers, 2008) During

Lab experiment

photosynthesis, carbon dioxide is taken up by cells and reduced to form various organic
molecules such as glucose. (Lambers, 2008)
The equation is as follows;
6CO2 + 6H2O + light  C6H12O6 + O2
The purpose of this experiment is to use titration to measure the rate of CO2 production in
several organisms.
The hypothesis of the experiment is; the rates of carbon dioxide production are indirectly related
to the mass of the organism.
MATERIALS AND METHODS
Materials used
Five 150mL beakers

White background

Distilled water

Graduated cylinder

Balance

Light source

Watch

Buret

Flask of NaOH

Aluminum foil

Organisms- fish, Elodea
A dropping bottle of phenolphthalein (1% stock solution of the indicator dye)

Lab experiment

METHODS
I.

Calculate volume and setting up beakers

1. Five 150 ml beakers were obtained and labeled 1-5
2. 100mL of distilled water was added to beaker 1.
3. The beaker was placed on a balance and weighed then an organism was added to the
beaker. The mass of the organism was then determined and recorded. The mass was then
converted to volume and recorded on a table.
4. The same process was repeated for beakers 3-4, with the volume of the organisms
recorded in the table.
5. 100mL of distilled water was added to beaker 5. The beaker was then put aside to the end
of the experiment.
6. A light source was then p...


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