Genetics Lab report(Just need answers and results)

timer Asked: Jun 26th, 2018
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Question description

Need someone helps me do the lab report

There are TWO parts.

Chromosome Linkage Mapping & Restriction site mapping of a mitochondrial DNA molecule

Make sure you do all of them.

Does not require a formal lab report. Just follow the steps and give your answers or results.

All details are in the uploaded pdf file. Please read it carefully and follow it exactly as required.

Thank you so much!

Department of Biological and Environmental Sciences BIO L320 – Genetics Lab Dr. Jacqueline Jones Lab 6. Chromosome Linkage Mapping You have conducted some pioneering work in mapping the chromosomes of Martians. Martians show the WZ sex-determining mechanism, so you obtain the data in Table 1. By conducting test crosses of double heterozygous members of sex A with hemi-zygous recessive members of sex B for four Z-linked recessive traits in Martians: blue feet (bl) [recessive to wildtype green feet], bobbles on the head (bo) recessive to wild-type ridges on the head], five toes (ft) [recessive to the wild-type four toes], and warty-nose (wn) [recessive to wild-type normal nose]. Table 1. Recombinant proportions for four Z-linked gene pairs in Martians. Gene pairs # recombinants/total offspring Blue feet (bl) – bobbles (bo) 536/12,382 Blue feet (bl) – five toes (ft) 2026/6611 Blue feet (bl) – warty nose (wn) 1644/4646 Bobbles (bo) – five toes (ft) 105/409 Bobbles (bo) – warty nose (wn) 1415/4515 Five toes (ft) – warty nose (wn) 28/537 Frequency % = cM 1. What sex would members of sex A have been? (1 point) 2. What sex would members of sex B have been? (1 point) 3. What are the distances between each of the gene pairs in Table 1 in centrimorgans (cM)? (9 points, ½ each for frequencies and 1 each for distances in cM) 4. What are the relative orders of these genes on the Martian Z chromosomes? (4 points) 5. Construct a linkage map of the Martian Z chromosome below showing the relative locations of these genes along it with relative locations in centrimorgans (cM) (5 points). Total = 20 points.
Department of Biological and Environmental Sciences BIO L320 – Genetics Lab Dr. Jacqueline Jones Lab 6. Restriction site mapping of a mitochondrial DNA molecule You have isolated some mitochondrial DNA (mtDNA) from walleye (Sander vitreus). The mtDNA was digested with four restriction endonucleases Apa I, Bcl II, Cla I, and Dra I). The fragments obtained (sizes in kilobase pairs) with each enzyme were as follows: Apa I 11.5, 3.0, 2.0 Bcl II 1 cut only – fragment size 16.5 Cla I 11.3, 5.2 Dra I 13.0, 3.5 You wish to map these restriction sites on the molecule, so you conduct a series of double digests, which give the following sized fragments: Apa I/Bcl II 10.0, 3.0, 2.0, 1.5 Apa I/Cla I 7.5, 4.0, 2.0, 1.8. 1.2 Apa I/Dra I 9.5, 3.0, 2.0, 1.5, 0.5 Bcl II/Cla I 6.0, 5.3, 5.2 Bcl II/Dra I 13.0, 3.0, 0.5 Cla I/Dra I 5.5, 5.2, 3.5, 2.3 Draw a linear restriction site map for this Daphnia mtDNA that is opened at the single Bcl II restriction site. Show your working, including alternative positions of restriction sites in the preliminary maps. A polymorphism is now found using Dra I such that the new fragment pattern for this enzyme becomes: 8.0, 5.0, 3.5. What has happened? You conduct two more double digests in order to map this variable site, giving results as follows: Cla I/Dra I 5.2, 5.0, 3.5, 2.3, 0.5 Apa I/Dra I 5.0, 4.5 3.0, 2.0, 1.5, 0.5 Map the variable site and mark it with an *. You examine another individual with Dra I revealing the following fragment results: 9.0, 5.0, 3.5. You think that this is a bit odd. So you conduct a few more digests, getting the following results: Apa I 11.5, 4.0, 2.0 Cla I 11.3, 6.2 Where is this phenomenon affecting the mtDNA molecule? Indicate the portion of the molecule that is affected. What fragments would you expect to result from a Cla I/Dra I double digest on this individual? Draw a picture of a gel showing all of the fragments obtained in the above digests. Total points = 50

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School: Cornell University


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Thanks, good work

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