Enzyme Lab Report

timer Asked: Oct 18th, 2018
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Question Description

Hello, I need a lab report done for my Biology class. The lab is on Enzymes. You will need to READ the pages of the pre-lab that I will attach to this and the lab that I completed myself and write a lab report on it. I will attach the requirements to EACH part of the lab report as well to this. Lab report should have a Introduction, Methods, Results, and Discussion. Each parts requirements need to be completed as it is stated. All the data and everything you need will be attached to this to write the lab report. Do not plagiarize from anywhere or do not use any outside sources for any of the questions besides the lab pages I post. If you have any questions please let me know! Thank you!

Enzyme Lab Report
Enzyme Lab Report
Enzyme Lab Report

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Running Head: ENZYME LAB i Enzyme LAB Presented by #NAME In Partial Fulfillment of the Requirements for #INSTITUTION #COURSE October 8, 2018 ENZYME LAB 1 Enzyme LAB Pre-Lab Write-up Hypothesis: If we cool the amylase solution down to 4 degrees Celsius, the enzymatic process will decrease, thus fewer molecules of starch will be disintegrated than a 24 degrees Celsius amylase solution. Null Hypothesis (H0): There will be no significant change in efficacy of the enzyme, as temperature decreases. Alternative Hypothesis (Ha): Amylase at 24 degrees will be more efficient than amylase at 4 degrees Celsius. The single variable that will change between test tubes 2 and 3 is temperature, because we need the same quantity of amylase and starch in each tube, for the experiment to be accurate. So the temperature will be the only independent variable. Prediction: Test tube 2 with a temperature of 14 degrees will exhibit a higher level of enzyme activity than test tube 3 with a temperature of 4 degrees. The component omitted from the positive control, with a zero expected absorbance will be amylase, and the component omitted from the negative control, expected to have zero absorbance is the starch. So to zero the absorbance we will place tube #1 in the spectrophotometer. The buffer is needed for the control tubes 1 and 4 to prevent amylase denaturation due to pH fluctuations. ENZYME LAB 2 Table 4. Experimental Design Tube # 1: Negative 2 3 4: Positive control 14°C 4°C Room temperature control Temperature Room temperature (24°C) (24°C) Starch (ml) 0 ml 1 ml 1 ml 1 ml Amylase (ml) 1 ml 1 ml 1 ml 0 ml Buffer (ml) 1 ml pH 7.5 1 ml pH 7.5 1 ml pH 7.5 1 ml pH 7.5 Total (ml) 2 ml 3 ml 3 ml 2 ml ...
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Tutor Answer

School: Carnegie Mellon University


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Tutor went the extra mile to help me with this essay. Citations were a bit shaky but I appreciated how well he handled APA styles and how ok he was to change them even though I didnt specify. Got a B+ which is believable and acceptable.

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