Complete Biology Lab

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Name: Per. Enzyme Time! WebQuest and Video Game Prior Knowledge: In this lesson you will discover how enzymes help our bodies to break down or build up a specific substrate and catalyze chemical reactions. You will learn how pH, temperature, and concentration affect the rate of chemical reactions in our bodies, and will experiment using graphs and interactive games to learn and check your understanding of enzymes. Before beginning, use the Learning Scale below to rate your knowledge of enzymes. Place a check in the Before Lesson box. Re-rate yourself in the Rating After Lesson box after the lesson. Rating Before Lesson Rating After Lesson Learning Scale 4 - I can teach others about how enzymes are specified proteins that catalyze chemical reactions and how they can be denatured by non-optimal conditions. 3 - I can explain how enzymes are specified proteins that catalyze chemical reactions and how they can be denatured by non-optimal conditions. 2 - I can describe how enzymes affect chemical reactions and how they can become denatured. 1 - I can identify the significance of enzymes in chemical reactions. 0 - I do not understand enzymes or how they can become denatured. Task 1: Introduction to Enzymes Discover how enzymes function. Click on the following link to watch the “Enzymes and Pac-Man” video by the Amoeba Sisters. As you watch, answer the questions below. Tiny URL: http://tinyurl.com/jkv9clr https://www.youtube.com/watch?annotation_id=annotation_4145881127&feature=iv&src_vid=XUn64HY5bug&v=qgVF kRn8f10 1. What is the name of the site where the substrate binds to the enzyme? 2. What is a substrate? 3. What is induced fit? 4. How do enzymes change the speed of a chemical reaction? 5. What two factors can denature enzymes? 6. How is an enzyme’s shape affected when it becomes denatured? 7. Why do lactose intolerant people get sick from drinking milk? © Science Is Real by Ana Ulrich Task 2: Enzymatic Virtual Lab Game Click on the link the below to play the “Enzymatic” virtual lab video game from the BioMan Biology website. Click “Start a New Game” to begin. Read the screens and follow the directions to complete the game. As you complete the game, answer the following questions on the handout. Tiny URL: http://tinyurl.com/mbstogk Long URL: https://biomanbio.com/HTML5GamesandLabs/LifeChemgames/enzymatichtml5 page.html Part A: What Are Enzymes? - Click on “What Are Enzymes” tab. 1. Enzymes are that speed up or chemical reactions. 2. Enzymes are important because all living things must do many in order to survive. 3. The chemical reactions done by living organisms are known as 4. In biology, the two most important types of reactions are 5. and is a type of chemical reaction that involves the 6. together building blocks called of polymers into is a reaction that involves large molecules by joining 7. Dehydration synthesis happens in your cells when you need to build large molecules such as , , or , Part B: Specificity of Enzymes - Click on the “Specificity” tab. 8. Each enzyme has a unique that makes an 9. Each active site can only fit a specific 10. When a substrate(s) enters the active site, the enzyme helps a specific to occur. The end result of a chemical reaction is the or 11. What is the name of the part of the enzyme where the substrate binds and causes a chemical reaction to occur? 12. We say that enzymes are specific. What does this mean? Part C: Experimenting With Enzymes – Click on “Experiments” tab. 13. What three factors affect an enzyme’s capability to catalyze chemical reactions? © Science Is Real by Ana Ulrich 14. Use the slider to adjust the temperature to a low (not the lowest) setting. What do you notice about the rate of reaction when the temperature is low? 15. Use the slider to adjust the temperature to a medium setting. Describe the rate of enzyme activity. 16. Use the slide to adjust the temperature to the high setting. What happened to your enzyme? 17. What does becoming “denatured” mean? 18. What is the temperature that an enzyme works best at is called? 19. What is the optimal temperature of this enzyme? 20. The is used to tell how acidic or basic a solution is. 21. Temperature damage can denature an enzyme permanently. Does extreme pH changes permanently denature an enzyme? Explain. 22. What is the optimal pH range of the stomach enzyme pepsin? 23. Substrate refers to the relative amount of substrate in a solution. 24. What does a high substrate concentrate mean? 25. What does a lower substrate concentration mean? 26. What is your hypothesis that you typed about substrate concentration and enzyme activity? 27. Why is there more enzyme activity at a higher substrate concentration than a lower substrate concentration? 28. Which factors can cause an enzyme to denature? Part D: Build an Enzyme! Draw enzyme and substrate here. 29. In the box to the right, draw the enzyme and substrate you will be experimenting with. 30. What is the optimal temperature of your enzyme? What is the optimal pH? 31. What is the enzymes optimal substrate concentration? 32. Observe your machine; describe what did you see. 33. Go to the main menu and click on the “Quiz!” tab to take the quiz. What score did you receive? For full credit, include a screenshot of your all your quiz grades along with this document. Quiz grades can be viewed by clicking on “Go to score sheet”. © Science Is Real by Ana Ulrich

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DasilvaPaul
School: University of Virginia

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