biochem fill out

timer Asked: Oct 24th, 2018
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Question description

I have some biochemistry questions, that you'll answer from powerpoints that I'll provide. I don't need an essay answers, I need like a bullot points (I'll send an example of how it should looks like) that's it.

Tutor Answer

School: Carnegie Mellon University


Running head/ questions and answers



Running head/ questions and answers


CHAPTER 5 - Exploring Genes and Genomes: Tools employed and how each works.

Know functions, source and examples of restriction enzymes and their recognition


Functions of restriction enzymes is to cleave DNA at highly specific sequences.


Its source is from bacteria enzymes


Their recognition sequences


What is gel electrophoresis? What are 2 main types and when each is appropriate?


Gel electrophoresis is a technique used to separate DNA fragments according to their size


2 main types are agarose and is appropriate when DNA fragments generated by

restriction enzyme digestion need to be separated.

Polyacrylamide is appropriate in the electrophoretic separation of nucleic acids

Running head/ questions and answers


Know the two commonly used stains, their advantages and disadvantages.


Methylene blue and iodine there advantages are simple and cost-effective, gives quick


results when examining infections, and basically key procedure in identifying bacteria. The
disadvantages are has few side effects, and It has significant limitations when used for
environmental microbiology.

Know applications of restriction enzyme digestion and gel electrophoresis


Application is demonstrating how DNA can be specifically cut into fragments by

restriction of enzymes and then can be separated by fragment size on an agarose gel.

What is Southern blotting? why is it necessary? How is it different from Northern &

Western blots?

Is process of transferring the separated fragments to nitrocellulose paper and that makes

it necessary.

The difference between them is that southern is used to find out unknown DNA sample


Northern used to find out target RNA


Western used to detect protein samples from mixture of other proteins.


Be able to analyze forensic gel electrophoresis.


Gel electrophoresis used in forensic science is a way to analyze DNA. In the case of a

crime, the ...

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