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Mounting and labeling

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Our Lady of Fatima University
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HPCT
MOUNTING and LABELING
3
RD
YEAR | 1
ST
SEMESTER | PRE-FINALS | LABORATORY LESSON 03 WEEK 03
CORPUZ
MOUNTING
• Mounting is the last step in tissue processing.
• The result of this process is that the mounting
medium bonds with the slide, cover slip and the
tissue specimen
• A mounting medium is usually a syrupy fluid and
gives a firm adhesion to the coverslip and the slide
• Mounting media are chosen based on refractive
index.
Characteristics of a good mounting medium:
1. It should be colorless and transparent.
2. It should be freely miscible with xylene and
toluene.
3. It should not dry to a non-stick consistency and
harden relatively quickly.
4. It should protect the section from physical
damage and chemical activity (oxidation and
changes in pH).
5. It should be resistant to contamination
(particularly microorganism growth).
6. It should not cause shrinkage and distortion of
tissues.
7. It should not leach out any stain or affect staining.
8. It should not change in color or pH.
9. It should be compatible with the adhesive in use.
MOUNTING MEDIA
• Aqueous media
• Resinous Media
Aqueous
• Water
o low refractive index
o good only for temporary mounting
• Glycerin
o used as a preservative.
o refractive index of 1.46
o regarded as the standard mountant for
fat stains.
• FARRANT'S MEDIUM
o Refractive Index 1.43
• APATHY'S MEDIUM
o Refractive Index 1.52 used for methylene
blue-stained nerve preparations and as a
general purpose aqueous mountant
o does not require ringing.
• BRUN'S FLUID
o for mounting frozen sections from water.
Resinous media
• Canada Balsam
o Refractive Index 1.524
o natural resin extracted from the
Canadian tree, Abus Balsamea
• DPX - (Dibutyl Phthalate and Xylene)
o Refractive Index 1.532
o recommended for small tissue sections
but not for whole mounts because of
shrinkage produced on drying.
• ХАМ
o Refractive Index 1.52
• CLARITE
o Refractive Index 1.544
o Synthetic resin generallpreffered than
DPX

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HPCT MOUNTING and LABELING 3RD YEAR | 1ST SEMESTER | PRE-FINALS | LABORATORY LESSON 03 WEEK 03 CORPUZ • • • • 1. 2. 3. 4. 5. 6. 7. 8. 9. • • MOUNTING Mounting is the last step in tissue processing. The result of this process is that the mounting medium bonds with the slide, cover sli ...
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