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Biosensors and Bioelectronics 67 (2015) 739–746 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: www.elsevier.com/locate/bios Surface-enhanced Raman spectroscopy detection of dopamine by DNA Targeting amplification assay in Parkisons's model Jeung Hee An a, Dong-Kug Choi b, Kwon-Jai Lee a,c, Jeong-Woo Choi d,n a Division of Food Bioscience, Konkuk University, Chungju-si 380-701, South Korea Department of Biotechnology, Konkuk University, Chungju-si 380-701, South Korea c Department of Advanced Materials Engineering, Daejeon University, Daejeon 300-716, South Korea d Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, South Korea b art ic l e i nf o a b s t r a c t Article history: Received 5 June 2014 Received in revised form 20 October 2014 Accepted 20 October 2014 Available online 23 October 2014 Dopamine is a potent neuromodulator in the brain that influences a variety of motivated behaviors and is involved in several neurologic diseases. We evaluated a bio-barcode amplification assay for its ability to detect dopamine in a mouse model with and without prior administration of the neurotoxin 1-methyl-4phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP). Our approach uses a combination of DNA barcodes and bead-based immunoassays for detecting neurotransmitters with surface-enhanced Raman spectroscopy (SERS). This method relies on a gold nanoplate with adsorbed antibodies and gold nanoparticles tha ...
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