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Why the Ice Cooled Enzyme Did Not Produce Reaction Strength Questions

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Conclusions
1. Explain why the ice-cooled enzyme did not produce reaction strength
like the normal or untreated enzyme.
- Cooling with the use of ice the enzyme will slow down its ability to
decompose the hydrogen peroxide into water and oxygen gas. This will lead
to fewer bubble production compared to the untreated setup.
2. Explain the difference between air-cooled and ice-cooled enzyme as both
had been pre-heated but one showed a bit more activity than the other.
- Ice-cooling the enzyme involves removing heat from it that is responsible
for rearranging the molecules of enzyme and by this, once hydrogen
peroxide is added, reaction will not be slowed because there is lesser energy
involved. Meanwhile, air-cooling the enzyme would not reduce its ability to
decompose the added enzyme as it has enough amount of heat needed to
affect all molecules of enzymes and therefore, the reaction would be a blast
that it will greatly decompose the hydrogen peroxide, producing more
bubbles.
3. Explain why the optimal enzyme concentration was not the strongest
concentration as evidence in the graph.
- In binding the substrate and enzyme, best concentration and not the strongest
one is being taken in consideration. In real life, enzymes in our body has a
specific pH to maintain so in order to maintain the pH, the right amount of
enzyme or concentration is needed and not the strongest. This is important to
maintain the equilibrium of the body.
4. Explain the concept of Enzyme Saturation Point and what happens to
the Vmax as the [S] continues to rise.
- Saturation point of enzyme is the maximum concentration an enzyme could
react. That point in the graph is the intersection of Vmax and best fit of line.
When [S] continues to rise, the Vmax will decrease as it reaches already the
maximum concentration it can hold.
5. How can you when apply the Michaelis-Menten dynamics to explain
what happens plasma concentration of solute reached Tm and the effect
that has on Clearance of the solute?
- Similar to the concept of saturation point in that dynamics, the plasma has a
saturation point which is the Tm that when we reached it, the plasma
reaction with other enzymes is greatest at that point and rate of reaction is

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fastest. The clearance of solute in the body is equivalent to the ratio of mass
removal rate to blood solute concentration. The effect of this is that reaching
the Tm will give the lowest value of solute clearance.
6. Explain how Km value can be used compare the effectiveness of drugs
that are competitive enzyme inhibitors. Would Km apply to non-
competitive inhibitors?
- Km value is higher when the drug has competitive inhibitor. This is because
the competitive inhibitor reduces the amount of active enzyme at lower
concentrations which leads to more substrate that will supply the reduced
amount of enzyme sufficiently to get to ½ of Vmax. Non-competitive
inhibitor reduces the amount of enzyme to the same fixed amount in a
certain experiment. So here, KM value does not change or constant from that
uninhibited reactions. KM would not actually apply on non-competitive
inhibitors when it comes to comparison.

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Conclusions 1. Explain why the ice-cooled enzyme did not produce reaction strength like the normal or untreated enzyme. - Cooling with the use of ice the enzyme will slow down its ability to decompose the hydrogen peroxide into water and oxygen gas. This will lead to fewer bubble production compared to the untreated setup. 2. Explain the difference between air-cooled and ice-cooled enzyme as both had been pre-heated but one showed a bit more activity than the other. - Ice-cooling the enzyme involves removing heat from it that is responsible for rearranging the molecules of enzyme and by this, once hydrogen peroxide is added, reaction will not be slowed because there is lesser energy involved. Meanwhile, air-cooling the enzyme would not reduce its ability to decompose the added enzyme as it has enough amount of heat needed to affect all molecules of enzymes and therefore, the reaction would be a blast that it will greatly decompose the hydrogen peroxide, producing more bubbles. 3. Explain why the optimal enzyme concentration was not the strongest concentration as evidence in the graph. - In binding the substrate and enzyme, best concentration and not the strongest one is being taken in consideration. In real life, enzymes in our body has a specific pH to maintain so in order to maintain the pH, the right amount of enzyme or concentration is needed and not the strongest. This is important to maintain the equilibrium of the body. 4. Explain the concept of Enzyme Saturation Point ...
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