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Liquid-liquid Extraction Questions and Answers
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Liquid-liquid Extraction Questions and Answers
Part A: Solubility Testing with Colored Dyes
1. Describe the procedure in part A.
The aim of this activity is to identify how each molecule of Sudan blue, Sudan orange and Congo red might
change if they are dissolved in aqueous solutions that have different PH values. The PH solutions used are;
Water, 1M HCL, 1M NaOH. Everything will have to be dissolved in Hexane.
Procedure:
Take nine test tubes and divide them into three set, with each set containing three test tubes. Add a small
amount of Hexane to each test tube. Start the first experiment using Sudan blue. First take the first set and add
two to three drops of sodium blue and shake well to ensure that it mixes well with the Hexane. To the first
test tube, add some drops of water and shake, to the second tube, add some drops of 1M HCL and shake, to
the third tube, add some drops of 1M NaOH and shake. Observe what happens to each tube. Repeat this
procedure using Sudan orange and Congo red and take the same observations.
2 Summarize the results in table format. Draw the structure of the dye in either the organic or aqueous
layer. Carefully show a neutral or charged structure as would be expected in each layer. If nothing is in
the organic layer or aqueous layer for a particular test, put an X in that box.
Sudan Blue
X
Hexanes and Water
Hexanes and Aqueous NaOH
Hexanes and Aqueous HCl
Organic Layer
Organic Layer
Organic Layer
X HN--> H2N +
X
Soluble in organic layer
Aqueous Layer Aqueous Layer Aqueous Layer
X
X
X

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Liquid-liquid Extraction Questions and Answers Student’s Name Course Date Liquid-liquid Extraction Questions and Answers Part A: Solubility Testing with Colored Dyes 1. Describe the procedure in part A. The aim of this activity is to identify how each molecule of Sudan blue, Sudan orange and Congo red might change if they are dissolved in aqueous solutions that have different PH values. The PH solutions used are; Water, 1M HCL, 1M NaOH. Everything will have to be dissolved in Hexane. Procedure: Take nine test tubes and divide them into three set, with each set containing three test tubes. Add a small amount of Hexane to each test tube. Start the first experiment using Sudan blue. First take the first set and add two to three drops of sodium blue and shake well to ensure that it mixes well with the Hexane. To the first test tube, add some drops of water and shake, to the second tube, add some drops of 1M HCL and shake, to the third tube, add some drops of 1M NaOH and shake. Observe what happens to each tube. Repeat this procedure using Sudan orange and Congo red and take the same observations. 2 Summarize the results in table format. Draw the structure of the dye in either the organic or aqueous layer. Carefully show a neutral or charged structure as would be expected in each layer. If nothing is in the organic layer or aqueous layer for a particular test, put an X in that box. Sudan Blue X Hexanes and Water Organic Layer Hexanes and Aqueous NaOH Organic Layer X He ...
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