Access over 20 million homework & study documents

Forensic Analysis Of Dna

Content type
User Generated
Subject
Biology
School
University of Florida
Type
Homework
Rating
Showing Page:
1/5
Running head: DISCUSSION QUERIES 1
Discussion Queries
Name of the Student
Institution Affiliation

Sign up to view the full document!

lock_open Sign Up
Showing Page:
2/5
DISCUSSION QUERIES 2
Discussion Queries
Question #1
The final 60 minutes extension is purposed to assist in the initiating process of the
addition of untemplated products of A with its products of 3. A final rest is then provided to
assist the enzymes to complete its untemplated polyadenylation. However, when sufficient time
is not provided to add adenosine to every copy of PCR, there will be a few fragments that are one
base pair shorter compared to others. Lack of sufficient during down-streaming could generate
issues of data interpretation (Alaeddini, 2018).
Question #2
A primer that is well-designed forms the most significant element of a good PCR
reaction. Primers are synthesized chemically by the union nucleotides. Three main steps which
followed when designing PCR that includes denaturation, annealing, and extension. The first
step involves the denaturing of genetic material and converting the double-stranded molecule to
a single-stranded molecule (Lee, 2018). It is then followed by annealing of the primers to
complementary regions of a single-stranded molecule. The last step involves extension through
the action of the polymerase of DNA. The most preferred temperature for each process is 940C,
600C, and 700C respectively. Mainly because all the steps followed are highly sensitive to
temperature. Other properties considered when designing a PCR prime include:
18-24 bases of length
40-60% content of G/C
Begin and end with 1-2 G/C for each pair

Sign up to view the full document!

lock_open Sign Up
Showing Page:
3/5

Sign up to view the full document!

lock_open Sign Up
End of Preview - Want to read all 5 pages?
Access Now
Unformatted Attachment Preview
Running head: DISCUSSION QUERIES Discussion Queries Name of the Student Institution Affiliation 1 DISCUSSION QUERIES 2 Discussion Queries Question #1 The final 60 minutes extension is purposed to assist in the initiating process of the addition of untemplated products of A with its products of 3. A final rest is then provided to assist the enzymes to complete its untemplated polyadenylation. However, when sufficient time is not provided to add adenosine to every copy of PCR, there will be a few fragments that are one base pair shorter compared to others. Lack of sufficient during down-streaming could generate issues of data interpretation (Alaeddini, 2018). Question #2 A primer that is well-designed forms the most significant element of a good PCR reaction. Primers are synthesized chemically by the union nucleotides. Three main steps which followed when designing PCR that includes denaturation, annealing, and extension. The first step involves the denaturing of genetic material and converting the double-stranded molecule to a single-stranded molecule (Lee, 2018). It is then followed by annealing of the primers to complementary regions of a single-stranded molecule. The last step involves extension through the action of the polymerase of DNA. The most preferred temperature for each process is 940C, 600C, and 700C respectively. Mainly because all the steps followed are highly sensitive to temperature. Other properties considered when designing a PCR prime include: • 18 ...
Purchase document to see full attachment
User generated content is uploaded by users for the purposes of learning and should be used following Studypool's honor code & terms of service.

Anonymous
Just what I needed. Studypool is a lifesaver!

Studypool
4.7
Indeed
4.5
Sitejabber
4.4