# Bio120 Labex10 Photosynthesis 2

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Bio 120 Lab Exercise 10 Photosynthesis
Exercise 10
PHOTOSYNTHESIS
Student Learning Outcomes
At the completion of this exercise you should:
(1) Be able to define the following linear metric system units: meter, centimeter,
millimeter, micrometer, nanometer, and Angstrom.
(2) Be able to describe, using both nanometers and color descriptions, the wavelengths of
sunlight that the human eye can see.
(3) Be able to describe how the “paper chromatography” technique can be used to separate
out the different pigments present in plant leaves.
(4) Be able to describe, using both nanometers and color descriptions, the most important
wavelength of sunlight used in photosynthesis.
(5) Be able to describe an experimental design that could be used to measure the
relationship between light intensity and the rate of photosynthesis.
(6) Be able to diagram the summary equation for photosynthesis.
I. The Nature of Light Striking the Surface of a Leaf
Sunlight is a relatively small but very important part of the vast electromagnetic spectrum of
energy. Visible light, together with a small amount of invisible radiation at its boundaries, is the
only part of this spectrum which is useful to life. Close examination of visible natural light
reveals that it, too, contains a spectrum, which is revealed to our eyes in the colors of a rainbow.
Two physical properties of light are of special interest to biologists: wavelength and energy.
Procedure:

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Bio 120 Lab Exercise 10 Photosynthesis
1. Point the slit end of the #125 Wabash Spectroscope at a white light source, such as
a small light bulb. The prism within the spectroscope will separate the various
wavelengths (colors) of which the white light is composed.
2. The spectrum of visible light and the wavelengths of the colors can be seen when
you look through the eyepiece. Each white numbered marking denotes the
wavelength of the color above it. The wavelength is the actual length of a wave of
light of that color, measured in units called angstroms (Å).
Table 1. Metric System: Length Measurement Units
Quantity
Numerical Value
English Equivalent
Converting to Metric
kilometer (km)
1,000 m
1 km = 0.62 mile
1 mile = 1.609 km
meter (m)
100 cm
1 m = 3.28 feet
= 1.09 yard
1 yard = 0.914 m
1 foot = .305 m
centimeter (cm)
0.01 m
1cm = 0.394 inch
1 foot = 30.5 cm
millimeter (mm)
0.001 m
1 mm = 0.039 inch
1 inch = 2.54 cm
micrometer (1 µm),
also called a "micron (µ)"
0.000001 m
nanometer (nm),
also called a
"millimicron(mµ)"
0.000000001 m
angstrom (Å)
0.0000000001 m
*Note: In other sources, you will sometimes see another unit, the nanometer (nm), used to measure wavelength.
Each nanometer is equal to ten angstroms: for example, 7000 Å=700 nm. Although both the angstrom and the
nanometer are used to measure wavelength, the nanometer is currently the more frequently used unit.

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Bio 120 Lab Exercise 10 Photosynthesis Exercise 10 PHOTOSYNTHESIS Student Learning Outcomes At the completion of this exercise you should: (1) Be able to define the following linear metric system units: meter, centimeter, millimeter, micrometer, nanometer, and Angstrom. (2) Be able to describe, using both nanometers and color descriptions, the wavelengths of sunlight that the human eye can see. (3) Be able to describe how the “paper chromatography” technique can be used to separate out the different pigments present in plant leaves. (4) Be able to describe, using both nanometers and color descriptions, the most important wavelength of sunlight used in photosynthesis. (5) Be able to describe an experimental design that could be used to measure the relationship between light intensity and the rate of photosynthesis. (6) Be able to diagram the summary equation for photosynthesis. I. The Nature of Light Striking the Surface of a Leaf Sunlight is a relatively small but very important part of the vast electromagnetic spectrum of energy. Visible light, together with a small amount of invisible radiation at its boundaries, is the only part of this spectrum which is useful to life. Close examination of visible natural light reveals that it, too, contains a spectrum, which is revealed to our eyes in the colors of a rainbow. Two physical properties of light are of special interest to biologists: wavelength and energy. Procedure: Bio 120 Lab Exercise 10 Photosynthesis 1. Poi ...
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