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Microscopy And Cytology

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User Generated
Subject
Social Science
School
Collin County Community College
Type
Homework
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MICROSCOPY AND CYTOLOGY REPORT SHEET
Download the report sheet, complete it, and then
submit it via Blackboard email as an attachment. Do not change the format.
All blue shaded areas require answers.
I. Microscopy
Experiment (dry lab)
A. Locate the parts below and match them to their description.
ocular lens head
objective lens stage
condenser lens with filter light
revolving nosepiece base
coarse adjustment knob rheostat
fine adjustment knob arm
iris diaphragm
Name:
Unit #
A
Eyepiece
Ocular lens
B
Supports the ocular lenses
Head
C
Holds objectives that can be rotated
Revolving nosepiece
D
Connects the base and barrel
Arm
E
Lens that magnifies the specimen
Objective lens
F
Supports the slide
Stage
G
Focuses the light onto the specimen
Condenser lens with filler
H
Raises and lowers the stage for focusing
Coarse adjustment knob
I
Slightly moves the stage to sharpen the image
Fine adjustment knob
J
Illuminator
Light
K
Light control
Iris diaphragm
L
Supports the microscope
Base
M
Varies the intensity of the light
Rheostat

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B. Principles of microscopy
Experiment (dry lab)
Field of view
Lens
Ocular
lens
Total
Magnification
Diameter in mm
(3 significant
figures)
Diameter in
m
(3 sig figs)
Area in mm
2
A = r
2
( = 3.14)
(3 sig figs)
Scan Power
10X
40X
4.200
4200
13.85
Low Power
10X
100X
1.680
1680
2.216
High Power
10X
400X
0.420
420
0.138
Oil Immersion
10X
1000X
0.168
168
0.022
Note:
• power of ocular lens does not change
• to determine total magnification use: lens power X ocular lens power
• to determine diameter in mm: 40x X 4.200/total mag = diameter in mm
• to convert mm to m: mm X 1000 = m
• to determine area: radius is ½ of diameter. Take radius and square it then multiply by
(pi)
What happens to the field of view as magnification increases?
(It increase, decrease or stay the same.)
-Depth of focus
What happens to the depth of focus as magnification increases?
(It increase, decrease or stay the same.).
-Image orientation
Select the letter ‘e’ as you would see through the ocular if it were mounted right side
up on the slide. (A, B, C or D.)
The field of view decreases
The depth of focus increases
C

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MICROSCOPY AND CYTOLOGY REPORT SHEET Name: Unit # Download the report sheet, complete it, and then submit it via Blackboard email as an attachment. Do not change the format. All blue shaded areas require answers. I. Microscopy Experiment (dry lab) A. Locate the parts below and match them to their description. ocular lens objective lens condenser lens with filter revolving nosepiece coarse adjustment knob fine adjustment knob iris diaphragm A B C D E F G H I J K L M head stage light base rheostat arm Eyepiece Supports the ocular lenses Holds objectives that can be rotated Connects the base and barrel Lens that magnifies the specimen Supports the slide Focuses the light onto the specimen Raises and lowers the stage for focusing Slightly moves the stage to sharpen the image Illuminator Light control Supports the microscope Varies the intensity of the light Ocular lens Head Revolving nosepiece Arm Objective lens Stage Condenser lens with filler Coarse adjustment knob Fine adjustment knob Light Iris diaphragm Base Rheostat B. Principles of microscopy Experiment (dry lab) Field of view Lens Lens Power Ocular lens Total Magnification Diameter in mm (3 significant figures) D ...
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