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Uv2

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UV-Vis Spectroscopy
Introduction
Ultraviolet-Visible spectroscopy is a type of absorption spectroscopy in which atoms
absorb light energy in the ultraviolet-visible spectral region. In this case, the non-bonding
electrons within a molecule absorb the light energy in the form of ultraviolet or visible light, get
excited, and move to a higher energy level in the anti-bonding molecular orbitals (Perkampus,
2013). Some electrons are easily excited while others are not depending on the energy difference
between the highest occupied molecular orbitals and the lowest occupied molecular orbitals
(Perkampus, 2013). As a result, UV-VIS spectroscopy is used to determine the qualitative and
quantitative properties of various analytes such as metal ions and organic compounds.
With UV-Vis, spectroscopy samples are mostly in solution form and therefore, sample
preparation is one aspect that needs close attention when running an analysis. The most
commonly used solvents are ethanol and water depending on the solubility of the compounds to
be analyzed (Hardesty, 2010). When using this method for the quantitative determination of
compounds, Beer Lambert's law plays a critical role since it gives us the relationship between
absorbance and concentration. The law states that the absorbance of a solution is directly
proportional to the concentration of the absorbing compound in the solution and the path length
(Hardesty, 2010).
Beer lamberts law is given by 𝑨=𝜺𝒃c where,
A= absorbance 𝜺= absorbance factor b= optical distance through sample c= concentration

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1 Student’s Name Tutor’s Name Course Date 2 UV-Vis Spectroscopy Introduction Ultraviolet-Visible spectroscopy is a type of absorption spectroscopy in which atoms absorb light energy in the ultraviolet-visible spectral region. In this case, the non-bonding electrons within a molecule absorb the light energy in the form of ultraviolet or visible light, get excited, and move to a higher energy level in the anti-bonding molecular orbitals (Perkampus, 2013). Some electrons are easily excited while others are not depending on the energy difference between the highest occupied molecular orbitals and the lowest occupied molecular orbitals (Perkampus, 2013). As a result, UV-VIS spectroscopy is used to determine the qualitative and quantitative properties of various analytes such as metal ions and organic compounds. With UV-Vis, spectroscopy samples are mostly in solution form and therefore, sample preparation is one aspect that needs close attention when running an analysis. The most commonly used solvents are ethanol and water depending on the solubility of the compounds to be analyzed (Hardesty, 2010). When using this method for the quantitative determination of compounds, Beer Lambert's law plays a critical role since it gives us the relationship between absorbance and concentration. The law states that the absorbance of a solution is directly proportional to the concentration of the absorbing compound in the solution and the path length (Hardesty, 2010). Beer lambert’s l ...
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