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Bios256 week 4 lab 10 influence of fluid intake on urine formation laboratory report

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BIOS256 Week 4 Lab: #10 Influence of Fluid Intake on Urine Formation Laboratory Report Activity 10: Influence of Fluid Intake on Urine Formation Name: Shamir Tyson Instructor: Professor Shen Date: November 24, 2013 PREDICTIONS 1. Urine output will be highest during: Water Loading 2. Urine osmolality will be lowest during: Dehydration 3. Plasma osmolality: increases with dehydration METHODS AND MATERIALS 1. Dependent variable: Urine and plasma values 2. Independent variable: fluid intake 3. Controlled variables: age, gender and weight/ Room temperature 4. Subjects were asked to refrain from alcohol and caffeine for a day before the start of the experiment. Explain why this is important. This is important as caffeine and alcohol could affect the outcome of the experiment especially these products could cause excretion of urine since they are diuretics and effect plasma work. 5. What technique was used to measure urine and plasma osmolality? The centrifuge was used to separate the plasma. The plasma and urine was then refrigerated and later accessed and placed in the osmometer for results RESULTS See Table 3: Urine Production Rate See Graph 1: Average Daily Urine Production Under Different Hydration States Type in the following AVERAGE urine production rate (L/day) • Normal • • Dehydrated Water Loaded Subject 1 Subject 2 Subject 3 Average Normal 1.98 1.96 1.82 1.92 Dehydrated 0.63 0.62 0.64 0.63 Water Loaded 7.59 7.18 7.48 7.42 1. Does dehydration increase, d ...
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