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Restriction Enzymes Cut DNA at Specific Sequences Questions

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Complete the following problems.
Restriction enzymes (REs), which cut DNA at specific sequences, are classic tools in molecular
biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences
by analyzing the fragments generated upon restriction digest, as in the example shown in Figure
1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected
the pMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI,
and XhoI), and analyzed the results on an agarose gel, shown below. Using the data you can glean
from this gel, answer the questions that follow.
1. What is the total size of the pMBBS plasmid in bp?
Answer: ____3000_____ bp
2. How many cut sites on the pMBBS plasmid does each RE have?
EcoRI: ___1___ BamHI: ____1___ XhoI: ___1___
3. If the pMBBS plasmid were digested with all three REs together, how many fragments would
be observed, and what would be the sizes (in bp) of these fragments?
Number of fragments: three

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Complete the following problems. Restriction enzymes (REs), which cut DNA at specific sequences, are classic tools in molecular biology. Because of their specificity in cutting DNA, REs can be used to "map" DNA sequences by analyzing the fragments generated upon restriction digest, as in the example shown in Figure 1. Your task is to study the circular plasmid, pMBBS, through restriction digests. You subjected the pMBBS plasmid to complete digestion by different combinations of three REs (EcoRI, BamHI, and XhoI), and analyzed the results on an agarose gel, shown below. Using the data you can g ...
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