CELL &TISSUE CULTURE Somatic Hybridization BEST FOR You O R G A N I C S C O M P A N Y BEST FOR You O R G A N I C S C O M P A N Y 3 STEPS FOR SOMATIC HYBERDIZATION IN PLANT CELLS BEST FOR You O R G A N I C S C O M P A N Y 4 Protoplast fusion will help in the combination of mitochondria and chloroplasts to result in a unique nuclearcytoplasmic genetic combination. BEST FOR You O R G A N I C S C O M P A N Y 5 Applications of hybrid cells      To study the control of cell division and gene expression. To investigate malignant transformations. To obtain viral replication. For gene or chromosome mapping . Production of monoclonal antibodies. BEST FOR You O R G A N I C S C O M P A N Y 6 Protoplast isolation Plasmolysed content of plant cell enclosed by plasma membranes. Isolation of protoplast Mechanical Enzymatic Mechanical Methods: BEST FOR You O R G A N I C S C O M P A N Y 8 Enzymatic Methods: BEST FOR You O R G A N I C S C O M P A N Y 9 Mechanical method: Used for vacuolated cells like onion bulb scale, radish and beet root tissues  Low yield of protoplast  Laborious and tedious process  Low protoplast viability  enzymatic methods: Use of enzymes to dissolve the cell wall.  High yield of protoplast  Easy to perform  More protoplast viability  10 ENZYMATIC METHOD ✓One step method (direct method) ✓Two- step method (sequential method) Somatic Hybridization Fusion of Protoplasts Spontaneou ...
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