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Dna Fingerprinting

Content type
User Generated
Subject
English
School
University of Alabama at Birmingham
Type
Homework
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Video One
1. What does the DNA in the vials look like?
The DNA vial resembles a tiny drop liquid at the bottom of the tube.
2. Did the DNA samples look different from each other?
The DNA samples looked the same.
3. Write down which DNA sample is placed into which color tube.
Crime Scene Sample =>
Suspect One => Blue
Suspect two => Orange
Suspect Three=> Purple
Suspect four => Red
Suspect five => Yellow
4. Other than DNA, what was added to each tube?
10 microliters of an enzyme mix were also added to the DNA samples.
5. What was the micropipette set to?
10 microliters
6. How long did the tubes have to incubate for?
The tubes were incubated for 45 min
Video Two
1. What was added to each tube after the incubation? Why was it added?
After incubation, 5 microliters of loading dye are added to each test tube to weigh down
the DNA and prevent it from floating.
2. What was the micropipette set to?
5 microliters
3. Write down which lane each DNA sample ended up in.
Crime Scene Sample => Lane 2
Suspect one => Lane 3
Suspect two => Lane 4
Suspect Three=> Lane 5
Suspect four => Lane 6
Suspect five => Lane 7

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4. Knowing what you know about DNA, which pole do you think it will travel towards: the positive
or the negative?
The DNA fragments tend to migrate to the positive pole. This is because DNA is
negatively charged and is attracted to the positively charged pole.
5. Which fragments (small or large) will end up closer to the wells and which ones will end up
further away?
Smaller fragments will move further away from the wells while the larger fragments end
up closer to the wells. Since all DNA fragments have similar mass charges, small
fragments migrate through the gel faster than large fragments.
6. How long does the gel run for?
The Gel runs for 30 min.
Results
1. What biological molecule was actually in each band on the gel?
Each band contains fragments of DNA from samples used in the laboratory
2. Why is there more than one band for each of the samples?
If a gel is stained with a DNA binding color, the DNA fragments can be considered as
bands each of which constitutes a group of identical DNA fragments.
3. What caused the DNA to become fragmented?
The enzyme mix is used to cut the DNA into fragments
4. Which sample had the smallest DNA fragment? Explain.
Sample four and sample five has the smallest DNA fragments. The fragments travelled
further from the wells compared to other samples.
5. What would the gel have looked like if the DNA were not fragmented?
If the DNA were not fragmented, the gel would look like a single band.
6. What determines where a restriction enzyme will cut?
The number of cuts made in the DNA of an organism by a specific restrictive enzyme
depends on the number of restriction sites of that organism's DNA. A piece of DNA
formed by a couple of adjacent cuts is called a fragment restriction.
7. Based off of your gel, which suspect left their DNA at the crime scene? Explain.

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Video One 1. What does the DNA in the vials look like? • The DNA vial resembles a tiny drop liquid at the bottom of the tube. 2. Did the DNA samples look different from each other? • The DNA samples looked the same. 3. Write down which DNA sample is placed into which color tube. • Crime Scene Sample => • Suspect One => Blue • Suspect two => Orange • Suspect Three=> Purple • Suspect four => Red • Suspect five => Yellow 4. Other than DNA, what was added to each tube? • 10 microliters of an enzyme mix were also added to the DNA samples. 5. What was the micropipette set to? • 10 microliters 6. How long did the tubes have to incubate for? • The tubes were incubated for 45 min Video Two 1. What was added to each tube after the incubation? Why was it added? • After incubation, 5 microliters of loading dye are added to each test tube to weigh down the DNA and p ...
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Anonymous
Very useful material for studying!

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