rev bras hematol hemoter. 2 0 1 5;3 7(5):296–301 Revista Brasileira de Hematologia e Hemoterapia Brazilian Journal of Hematology and Hemotherapy www.rbhh.org Original article Hemoglobin A2 values in sickle cell disease patients quantified by high performance liquid chromatography and the influence of alpha thalassemia Silvana Fahel da Fonseca a,∗ , Tatiana Amorim b , Antônio Purificação b , Marilda Gonçalves c , Ney Boa-Sorte b a b c Universidade de Brasília (UnB), Brasília, DF, Brazil Associação de Pais e Amigos dos Excepcionais (APAE), Salvador, BA, Brazil Fundação Oswaldo Cruz (FIOCRUZ), Salvador, BA, Brazil a r t i c l e i n f o a b s t r a c t Article history: Background: In sickle cell disease, the quantification of Hb A2 is important for the differential Received 10 March 2013 diagnosis between sickle cell anemia (Hb SS) and Hb S/␤0 -thalassemia. Accepted 11 May 2015 Objective: To determine Hb A2 levels as quantified by high performance liquid chromatogra- Available online 7 June 2015 phy in patients with sickle cell anemia (Hb SS) and with the SC hemoglobinopathy, with or without concomitant alpha thalassemia. Keywords: Methods: This is a retrospective study of 242 children aged between two and six years with Hemoglobin A2 diagnoses of Hb SS or Hb SC. The hemoglobin was evaluated using high performance liquid Alpha thalassemia chromatography and alpha thalassemia [3.7 kb deletion (−␣3.7 )] was detected by polymerase B ...
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