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Pakistan Entomologist Journal homepage: www.pakentomol.com STUDY THE PREVALENCE OF DENGUE MOSQUITO SPECIES AND VIRUS SEROTYPES IN MULTAN Muhammad Asrar1*, Muhammad Riaz Athar1, Shabab Nasir1, Syed Makhdoom Hussain1, Dilbar Hussain2, Muhammad Azeem1, Awais Ali Chatha1, Hussnian Jabbar1, Ghulam Hussain1 and Saddam Hussain1 1 Department of Zoology, Government College University, Faisalabad, Pakistan Entomological Research Institute, Ayub Agricultural Research Institute, Faisalabad, Pakistan 2 ARTICLE INFORMATION A BS TR A C T Received: February 02, 2017 Dengue, a mosquito instinctive viral septicity is considered as a major unrestricted problem worldwide. Dengue virus is associated with the properties of encased, positive strand RNA viruses. Around 50 million individuals are at danger with 24,000 passing away year after year globally. In Pakistan during the most recent few years dengue has become an evolving vector endured disease. Dengue infection is triggered by DENV1, DENV2, DENV3, DENV4 and DENV5. Present study was carried out to identify the dengue mosquito species and virus serotypes prevailing in Multan city. Fully-grown mosquitos were sampled with aspirator and larvae with dipper from different nine areas of Multan city. Samples were well-preserved in 70% alcohol and carried to laboratory conditions in Department of Zoology, GC University Faisalabad. Mosquito were recognized and samples were kept at -20oC for PCR. Real time PCR were done to identify the presence o ...
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