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LAB 8 Report

LAB 8 Report

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MITOSIS & THE MITOTIC CELL CYCLE

INTRODUCTION

GENERAL BACKGROUND

Reproduction is the vital property of life. Reproduction and growth of

organisms is dependent upon their property of cells to divide. There are two ways by

which cells divide. One is called mitosis while the other is called meiosis

Mitosis or mitotic cell division produces two cells that are genetically

identical to each other and to the parent cell. The series of events that a cell must

progress through during mitotic cell division are referred to as mitotic cell cycle. The

cycle is divided into two main stages: interphase and M phase, further divided into

stages corresponding to the completion of one set of activities and the start of the next.

These stages are prophase, prometaphase, metaphase, anaphase, and telophase.

During mitosis, the chromosomes, which have already duplicated, condense and

attach to spindle fibers that pull one copy of each chromosome to opposite sides of the

cell. [1] The result is two genetically identical daughter nuclei. The rest of the cell

may then continue to divide by cytokinesis to produce two daughter cells. [2]

Mitosis occurs only in eukaryotic cells. Prokaryotic cells, which lack a

nucleus, divide by a different process called binary fission. Mitosis varies between

organisms.

MOTIVATION

Cell division being the basis of life is the most exciting thing to witness.

PURPOSE

Purpose of this experiment was to appreciate the type of cells in various stages of mitosis and to

see their relative numbers to determine the duration of various phases of mitosis.

EXPERIMENTAL DESIGN AND PROCEDURE

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APPARATUS

A prepared slide of an onion root tip was obtained. It was viewed at low power (4 x

objective) to find the region with the most cells undergoing cell division. Then

microscope was switched to high power (40x) to study the appearances of the

individual nuclei. Condensed chromosomes were seen.

APPROACH

Cells in interphase were identified. Then cells in each phase were

identified. Using 400 x magnifications, slide was centered to concentrate on the

region of dense cell division. In this field, the number of cells at each stage of mitotic

cell cycle was counted.

Then a prepared slide labeled whitefish blastula was obtained. One cross

section of the slide was found and focused under low power objective (10x) Then I

moved on to high power (40x). A cell in interphase was identified. Then cells in each

phase were identified.

Data sheet was then completed and slides returned to the boxes where they

came from.

PRESENTATION OF RESULTS

Presentation of results was done by a frequency table and a pie chart.

RESULTS

Interphase

Prophase

Metaphase

Anaphase

Telophase

Total #

of cells

observed

100

% of my

total

Class

total

% of

class

total

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MITOSIS & THE MITOTIC CELL CYCLE INTRODUCTION GENERAL BACKGROUND Reproduction is the vital property of life. Reproduction and growth of organisms is dependent upon their property of cells to divide. There are two ways by which cells divide. One is called mitosis while the other is called meiosis Mitosis or mitotic cell division produces two cells that are genetically identical to each other and to the parent cell. The series of events that a cell must progress through during mitotic cell division are referred to as mitotic cell cycle. The cycle is divided into two main stages: interphase and M phase, further divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to spindle fibers ...
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