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LAB 8 Report

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MITOSIS & THE MITOTIC CELL CYCLE
INTRODUCTION
GENERAL BACKGROUND
Reproduction is the vital property of life. Reproduction and growth of
organisms is dependent upon their property of cells to divide. There are two ways by
which cells divide. One is called mitosis while the other is called meiosis
Mitosis or mitotic cell division produces two cells that are genetically
identical to each other and to the parent cell. The series of events that a cell must
progress through during mitotic cell division are referred to as mitotic cell cycle. The
cycle is divided into two main stages: interphase and M phase, further divided into
stages corresponding to the completion of one set of activities and the start of the next.
These stages are prophase, prometaphase, metaphase, anaphase, and telophase.
During mitosis, the chromosomes, which have already duplicated, condense and
attach to spindle fibers that pull one copy of each chromosome to opposite sides of the
cell. [1] The result is two genetically identical daughter nuclei. The rest of the cell
may then continue to divide by cytokinesis to produce two daughter cells. [2]
Mitosis occurs only in eukaryotic cells. Prokaryotic cells, which lack a
nucleus, divide by a different process called binary fission. Mitosis varies between
organisms.
MOTIVATION
Cell division being the basis of life is the most exciting thing to witness.
PURPOSE
Purpose of this experiment was to appreciate the type of cells in various stages of mitosis and to
see their relative numbers to determine the duration of various phases of mitosis.
EXPERIMENTAL DESIGN AND PROCEDURE

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APPARATUS
A prepared slide of an onion root tip was obtained. It was viewed at low power (4 x
objective) to find the region with the most cells undergoing cell division. Then
microscope was switched to high power (40x) to study the appearances of the
individual nuclei. Condensed chromosomes were seen.
APPROACH
Cells in interphase were identified. Then cells in each phase were
identified. Using 400 x magnifications, slide was centered to concentrate on the
region of dense cell division. In this field, the number of cells at each stage of mitotic
cell cycle was counted.
Then a prepared slide labeled whitefish blastula was obtained. One cross
section of the slide was found and focused under low power objective (10x) Then I
moved on to high power (40x). A cell in interphase was identified. Then cells in each
phase were identified.
Data sheet was then completed and slides returned to the boxes where they
came from.
PRESENTATION OF RESULTS
Presentation of results was done by a frequency table and a pie chart.
RESULTS
Interphase
Prophase
Metaphase
Anaphase
Telophase
Total #
of cells
#
observed
50
18
8
4
20
100
% of my
total
40
18
8
4
20
Class
total
% of
class
total

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MITOSIS & THE MITOTIC CELL CYCLE INTRODUCTION GENERAL BACKGROUND Reproduction is the vital property of life. Reproduction and growth of organisms is dependent upon their property of cells to divide. There are two ways by which cells divide. One is called mitosis while the other is called meiosis Mitosis or mitotic cell division produces two cells that are genetically identical to each other and to the parent cell. The series of events that a cell must progress through during mitotic cell division are referred to as mitotic cell cycle. The cycle is divided into two main stages: interphase and M phase, further divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are prophase, prometaphase, metaphase, anaphase, and telophase. During mitosis, the chromosomes, which have already duplicated, condense and attach to spindle fibers ...
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